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Journal ArticleDOI

An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels.

01 May 1970-Analytical Biochemistry (Anal Biochem)-Vol. 35, Iss: 1, pp 296-297
About: This article is published in Analytical Biochemistry.The article was published on 1970-05-01. It has received 73 citations till now. The article focuses on the topics: Polyacrylamide & Tritium.
Citations
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TL;DR: The results obtained from these experiments suggested that the glycoprotein is closely associated with myelin and that it is not in an unrelated contaminating structure.
Abstract: — Myelin was purified from rats which had been injected intracerebrally with radioactive fucose in order to label specifically the glycoproteins. Myelin contained a small amount of fucose-labelled glycoproteins in comparison to that in other subcellular fractions, but polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate revealed a unique pattern of radioactive glycoproteins dominated by a major peak. The same glycoprotein was not prominent in the other subcellular fractions which were examined. This major glycoprotein in the myelin fraction was also labelled after injection with [3H]glucosamine or N-[3H]acetylmannosamine. It was the most intensely staining myelin protein when gels were treated with periodic acid-Schiff reagents, an indication that, in terms of protein-bound carbohydrate, it is the major glycoprotein in the myelin fraction. The glycoprotein was present in myelin purified from rats ranging in age from 14 days to 14 months. Extensive recycling of the myelin through the purification procedures did not significantly reduce the amount of glycoprotein in the myelin. Double label experiments with [3H]fucose and [14C]fucose were used to compare glycoproteins in myelin purified from white and grey matter, respectively, and from mixed homogenates of myelinated and unmyelinated brain. The results obtained from these experiments suggested that the glycoprotein is closely associated with myelin and that it is not in an unrelated contaminating structure. Possible locations of the glycoprotein are discussed. They include the myelin membrane itself, the oligodendroglial plasma membrane, and the axolemma of myelinated axons.

210 citations

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TL;DR: Results suggest that the gamma subunit of CF1 plays an important role in regulation of ATP synthesis and hydrolysis in leaves and intact chloroplasts.

182 citations

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TL;DR: Electrophoretic comparison of the polypeptides labeled in the presence and absence of oxygen revealed that oxygen controlled the promitochondrial synthesis of a specific class of proteins, and this effect of oxygen was inhibited by glucose.

150 citations

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TL;DR: The processing from the precursor to the mature lipoprotein at a high temperature resulted in accumulation of a peptide that was most probably the intact signal peptide, which had many unique properties: being highly resistant to high temperature, having a wide optimum pH range, and being highly sensitive to detergents.

131 citations

References
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Journal ArticleDOI
TL;DR: Experiments with S35-labeled SDS suggest that the SDS-protein complex is a loose one and that exchange of SDS with the environment occurs at an exceedingly high rate.

190 citations

Journal ArticleDOI
TL;DR: Hyamine hydroxide and NCS appear to be capable of solubilizing soft tissues and many purified biological materials so that low-energy beta emitters (H 3 and C 14 ) can be counted with true solution efficiencies, as determined by internal standardization, channels ratio, etc as mentioned in this paper.

136 citations