Journal ArticleDOI
An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts
Bertrand C. Y. Collard,Bertrand C. Y. Collard,M. Z. Z. Jahufer,J. B. Brouwer,Edwin C. K. Pang +4 more
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TLDR
This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS.Abstract:
Recognizing the enormous potential of DNA markers in plant breeding, many agricultural research centers and plant breeding institutes have adopted the capacity for marker development and marker-assisted selection (MAS). However, due to rapid developments in marker technology, statistical methodology for identifying quantitative trait loci (QTLs) and the jargon used by molecular biologists, the utility of DNA markers in plant breeding may not be clearly understood by non-molecular biologists. This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS. This review has been specifically written for readers who have only a basic knowledge of molecular biology and/or plant genetics. Its format is therefore ideal for conventional plant breeders, physiologists, pathologists, other plant scientists and students.read more
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Genetic Dissection of Complex Traits
TL;DR: The current state of the genetic dissection of complex traits is summarized in this paper, which describes the methods, limitations, and recent applications to biological problems, including linkage analysis, allele-sharing methods, association studies, and polygenic analysis of experimental crosses.
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Marker-assisted selection: an approach for precision plant breeding in the twenty-first century
TL;DR: An overview of the advantages of MAS and its most widely used applications in plant breeding, providing examples from cereal crops and ways in which the potential of MAS can be realized are suggested.
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Marker-Assisted Selection in Plant Breeding: From Publications to Practice
Yunbi Xu,Jonathan H. Crouch +1 more
TL;DR: Fundamental issues remain to be resolved, particularly regarding complex traits, before marker-assisted selection realizes its full potential in public sector breeding programs, including the development of high throughput precision phenotyping systems for QTL mapping, improved understanding of genotype by environment interaction and epistasis, and development of publicly available computational tools tailored to the needs of molecular breeding programs.
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Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants
TL;DR: It is proposed that this method could be used in conjunction with RAPD markers for applications such as genetic analysis, bulked segregant analysis, and quantitative trait loci mapping, especially in laboratories with a preference for agarose gel electrophoresis.
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Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives
TL;DR: This paper comprehensively reviews the significance of plant genetic diversity (PGD) and PGR especially on agriculturally important crops; risk associated with narrowing the genetic base of current commercial cultivars and climate change; analysis of existing PGD analytical methods in pregenomic and genomic era; and modern tools available for PGD analysis in postgenomic era.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
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AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
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MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations
Eric S. Lander,Eric S. Lander,Philip Green,Jeff Abrahamson,Aaron Barlow,Mark J. Daly,Stephen E Lincoln,Lee Newburg +7 more
TL;DR: A computer package, called MAPMAKER, designed specifically for the construction of linkage maps in a number of organisms, including the human and several plants, and it is outlined the mapping strategies that have been used.
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Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
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Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results
Eric S. Lander,Leonid Kruglyak +1 more
TL;DR: Specific standards designed to maintain rigor while also promoting communication are proposed for the interpretation of linkage results in genetic studies under way for many complex traits.