Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis–banana interaction
Citations
74 citations
Cites methods from "Analysis of expressed sequence tags..."
...Traditional genome-wide analysis of gene expression of organisms under different conditions or, in the case of pathogens, at different life cycle stages, has mainly been carried out by microarrays, suppression subtractive hybridization (SSH), and cDNA-AFLP methods [9-12]....
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52 citations
Cites background from "Analysis of expressed sequence tags..."
...acuminata late stage compatible interactions has also recently been reported [23]....
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...Analysis of gene expression during banana-Mycosphaerella interactions has been limited to date [15,23,24], with our study, to the authors knowledge, the first massal transcriptome analysis for the M....
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34 citations
Cites background from "Analysis of expressed sequence tags..."
...Activation of genes related to phenylpropanoid pathway was reported in other compatible interactions, however defence-related metabolites and primary cell-wall thickening components derived from this pathway were not accumulated [29, 30]....
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26 citations
23 citations
References
70,111 citations
"Analysis of expressed sequence tags..." refers methods in this paper
...Sequences were submitted to the BlastN and BlastX programs (http://blast.ncbi.nlm.nih.gov/Blast.cgi) for homology searching (Altschul et al. 1997), using the GenBank non-redundant (NR) database....
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...Primer sets for quantitative (q)-PCR on genes selected from the cDNA libraries were designed with Primer3 software (Rozen and Skaletsky 2000) and Blastchecked for non-specific binding sites against public available Musa and M. fijiensis sequences (Altschul et al. 1997)....
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16,407 citations
"Analysis of expressed sequence tags..." refers methods in this paper
...Primer sets for quantitative (q)-PCR on genes selected from the cDNA libraries were designed with Primer3 software (Rozen and Skaletsky 2000) and Blastchecked for non-specific binding sites against public available Musa and M....
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...Primer sets for quantitative (q)-PCR on genes selected from the cDNA libraries were designed with Primer3 software (Rozen and Skaletsky 2000) and Blastchecked for non-specific binding sites against public available Musa and M. fijiensis sequences (Altschul et al. 1997)....
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5,074 citations
"Analysis of expressed sequence tags..." refers methods in this paper
...All sequences were evaluated into the CAP3 program (Huang and Madan 1999) with default parameters to obtain cluster contigs....
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3,721 citations
"Analysis of expressed sequence tags..." refers background in this paper
...Our findings strongly suggest that JA and ET signaling transduction pathways, which are generally correlated with resistance against necrotrophic pathogens (Glazebrook, 2005), are active during late stages of this compatible interaction....
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3,144 citations
"Analysis of expressed sequence tags..." refers methods in this paper
...This method is based on selective amplification of differentially expressed sequences within two cDNA populations, which overcomes the technical limitations of traditional subtraction methods (Diatchenko et al. 1996; Gurskaya et al. 1996)....
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...The SSH methodology described by Diatchenko et al. (1996) was used to create a library that theoretically contains a mixture of plant and fungal infection-specific genes....
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