Journal ArticleDOI
Analysis of factors responsible for the regeneration to intact cells from sphaeroplasts of Saccharomyces cerevisiae
J. Anjani Kumari,Tapobrata Panda +1 more
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TLDR
The regeneration of Saccharomyces cerevisiae cells from its sphaeroplasts were found to be influenced by a number of factors, including malt-extractglucose-yeast extract-peptone medium and growth medium.Abstract:
The regeneration of Saccharomyces cerevisiae, NCIM 3288, cells from its sphaeroplasts were found to be influenced by a number of factors. The most suitable conditions of regeneration were also dependent on growth medium, that is, using malt-extractglucose-yeast extract-peptone (MGYP) medium: mannitol 0.7 M, pH 6.5, 30 °C and using yeast extract-peptone-glucose (YPG) medium: sucrose 0.7 M, pH 5.0 and 30 °C. The maximum regeneration frequency was observed in YPG medium.read more
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Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion
J. Anjani Kumari,Tapobrata Panda +1 more
TL;DR: An attempt to produce ethanol from a cellulosic substrate by a single-stage process using intergeneric hybrids obtained from Trichoderma reesei QM 9414 and Saccharomyces cerevisiae NCIM 3288 fusants is described, showing the highest synthesis of ethanol from filter paper cellulose.
Journal ArticleDOI
RESEARCH PAPER Protoplast fusion of Rhizopus oryzae and Rhizopus microsporus for enhanced fumaric acid production from glycerol
TL;DR: In this article, the ability of Rhizopus oryzae and rhizopus microsporus strains to produce fumaric acid on glycerol as the sole carbon source in the medium was evaluated.
Journal Article
Protoplast fusion of Rhizopus oryzae and Rhizopus microsporus for enhanced fumaric acid production from glycerol
TL;DR: Individual Rhizopus fusants demonstrated a various ability to produce fumaric acid from 2.0% (w/v) of glycerol, with the most effective ones producing from 0.2 to 0.27 g@ g !
References
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Journal ArticleDOI
Formation of aberrant cell walls and of spores by the growing yeast protoplast.
Journal ArticleDOI
Actin is associated with the formation of the cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe.
TL;DR: It is demonstrated that actin is associated with initiation of cell wall formation, the proper deposition ofcell wall materials, and maintaining the normal morphology of reverting protoplasts.
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Cell Wall Formation in Regenerating Protoplasts of Schizosaccharomyces pombe: Study by High Resolution, Low Voltage Scanning Electron Microscopy
Masako Osumi,Naoko Yamada,Hiromi Kobori,Akiko Taki,Nobuko Naito,Misuzu Baba,Takashi Nagatani +6 more
TL;DR: It is suggested that beta-glucan is the main component of the microfibrils and that it plays an important role in the formation of the cell wall in S. pombe.
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Regeneration of Mycelial Protoplasts of Fusarium culmorum
TL;DR: Regeneration was greater under acid conditions, alkaline pH values interfering with the phenomenon, and agar was the best substance for regeneration; gelatin produced an inhibition.