scispace - formally typeset
Search or ask a question
Journal ArticleDOI

Analysis of phospholipids in membrane of Acidithiobacillus ferrooxidans grown in liquid (ferrous sulphate and sodium thiosulphate) and solid (sulfur) substrate

TL;DR: It is proved beyond doubt that when bacteria are exposed to different environments they modify the cell membranes to adapt to the new conditions and the first time that the PLs were not same in both liquid and solid substrate grown bacteria is demonstrated.
Abstract: Phospholipids (PLs) are the important components of cell membrane of all living organisms. The PL or the cell membrane of the bacteria are the least studied subject as it involves large quantities of material and few methods to assay. The novel study was designed to understand if the cell membrane remains same or it changes during different conditions. The bacteria, Acidithiobacillus ferrooxidans which can live as a planktonic as well as sessile form was chosen to study the difference in Phospholipids present in the cell membrane. The Acidithiobacillus ferrooxidans was grown in liquid substrate Sodium Thiosulphate and a solid substrate Sulfur keeping Ferrous Sulphate substrate grown bacteria as a control. The PLs were extracted using Bligh and Dyer method and concentrated by nitrogen stream drying and isolated using Thin Layer Chromatography (TLC). The isolated PLs were subjected to gas chromatography-mass spectrometry (GC-MS) to characterize the isolated PLs. The results show that Rf value of the PLs and the yield were different in Acidithiobacillus ferrooxidans grown in Ferrous Sulphate, Sodium Thiosulphate and Sulfur substrates. The present study has successfully demonstrated for the first time that the PLs were not same in both liquid and solid substrate grown bacteria. Hence it is proved beyond doubt that when bacteria are exposed to different environments they modify the cell membranes to adapt to the new conditions. The Biofilm forming planktonic Acidithiobacillus ferrooxidans bacteria had different PLs profile then sessile bacterial.

Content maybe subject to copyright    Report

Citations
More filters
Journal ArticleDOI
TL;DR: The alteration of fatty acid profile of brain of the mice suggests that the derivatizing technique can be applicable to most research fields associated with the environmental neurotoxins with better resolution in a short time, as compared to the current protocols for lipid analysis.

13 citations

References
More filters
Journal ArticleDOI
E. G. Bligh1, W. J. Dyer1
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Abstract: Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can...

46,099 citations

Journal ArticleDOI
TL;DR: Biological iron apportionment has been described as one of the most ancient forms of microbial metabolism on Earth, and as a conceivable extraterrestrial metabolism on other iron-mineral-rich planets such as Mars.
Abstract: Iron (Fe) has long been a recognized physiological requirement for life, yet for many microorganisms that persist in water, soils and sediments, its role extends well beyond that of a nutritional necessity. Fe(II) can function as an electron source for iron-oxidizing microorganisms under both oxic and anoxic conditions and Fe(III) can function as a terminal electron acceptor under anoxic conditions for iron-reducing microorganisms. Given that iron is the fourth most abundant element in the Earth's crust, iron redox reactions have the potential to support substantial microbial populations in soil and sedimentary environments. As such, biological iron apportionment has been described as one of the most ancient forms of microbial metabolism on Earth, and as a conceivable extraterrestrial metabolism on other iron-mineral-rich planets such as Mars. Furthermore, the metabolic versatility of the microorganisms involved in these reactions has resulted in the development of biotechnological applications to remediate contaminated environments and harvest energy.

1,440 citations

Journal ArticleDOI
TL;DR: A new genus and species, Alcanivorax borkumensis, is described to include six heterotrophic bacterial strains isolated from enriched mixed cultures, obtained from sea water/sediment samples collected near the isle of Borkum (North Sea), using Mihagol-S (C14,15-n-alkanes) as principal carbon source.
Abstract: During screening for biosurfactant-producing, n-alkane-degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea water/sediment samples collected near the Isle of Borkum (North Sea), using Mihagol-S (C14,15-n-alkanes) as principal carbon source. These Gram-negative, aerobic, rod-shaped bacteria use a limited number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and pyruvate and its methyl ether. During cultivation on n-alkanes as sole source of carbon and energy, all strains produced both extracellular and cell-bound surface-active glucose lipids which reduced the surface tension of water from 72 to 29 mNm-1(16). This novel class of glycolipids was found to be produced only by these strains. The 16S rRNA gene sequence analysis showed that these strains are all members of the γ-subclass of the Proteobacteria. Their phospholipid ester-linked fatty acid composition was shown to be similar to that of members of the genus Halomonas, although they did not demonstrate a close phylogenetic relationship to any previously described species. On the basis of the information summarized above, a new genus and species, Alcanivorax borkumensis, is described to include these bacteria. Strain SK2Tis the type strain of A. borkumensis.

530 citations

Journal ArticleDOI
TL;DR: An improved procedure for the quantitative assay of phospholipids separated by TLC is described, in which a specially washed Silica Gel H and a newly designed chromatography unit are employed.

365 citations