Analysis of the Lung Microbiome in the “Healthy” Smoker and in COPD
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Cites background from "Analysis of the Lung Microbiome in ..."
...Molecularbased studies of the LRT using bronchoscopic collection of BAL or mucosal brushes have reported that airways of healthy individuals contain a characteristic distribution of organisms, often at very high levels (10, 11)....
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...Traditional culture-based studies and classic teaching indicate that the healthy LRT is sterile (7–9), but recent cultureindependent techniques have suggested otherwise (10, 11)....
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Cites background from "Analysis of the Lung Microbiome in ..."
...Others have found few differences in the lower respiratory tract microbiome of smokers and nonsmokers, but these studies have examined small numbers of subjects (7)....
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...In comparisons between OW communities of nonsmokers and smokers using V1–3 sequence data, we measured significant differences in OTUs classified as Neisseria sp., Porphyromonas sp., and Gemella sp. (Figure 4A)....
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...Bronchoscopic contamination or carryover from the upper airway may also occur and influence detection of microbes in BAL samples, but studies of lung tissue have confirmed the independent presence of bacterial populations (7, 9)....
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...Porphyromonas, a bacteria linked to periodontal disease, is generally increased in smokers who show a decreased inflammatory response to this organism; however, we found that it was depleted in oral washes of smokers (14)....
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...Prevotella, Fusobacterium, Haemophilus, Veillonella, and Porphyromonas (7, 8)....
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578 citations
References
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"Analysis of the Lung Microbiome in ..." refers methods in this paper
...We categorized subjects using the spirometric classification of the Global Initiative for Chronic Obstructive Lung Disease (GOLD) [18]....
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16,048 citations
"Analysis of the Lung Microbiome in ..." refers methods in this paper
...Following quality control filtering of the sequences we used RDPClassifier [25] to assign taxonomic classifications to the sequences for ecological analysis....
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