Analyzing real-time PCR data by the comparative C(T) method.
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...CVs should not be used with Cqs, however ( 29 )....
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...CVs should not be used with Cqs ( 29 ) but can be used to express the variance in copy numbers or concentrations....
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...If multiple reactions are performed, however, accurate quantification of lower concentrations can be obtained via digital PCR ( 29 , 63, 64)....
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...As discussed above, reporting of CVs for Cqs is inappropriate ( 29 ), because Cqswillalwaysbelower(andthereforepotentiallymisleading) than CVs calculated for copy numbers....
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...The efficiency correction method calculates the relative expression ratio from the real-time PCR efficiencies and the C T (ref...
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"Analyzing real-time PCR data by the..." refers methods in this paper
...Most often in gene expression profiling experiments the data are presented as a heatma...
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"Analyzing real-time PCR data by the..." refers methods in this paper
...Absolute expression provides the exact copy number following transformation of the data via a standard curv...
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