Antibacterial and antioxidant activity of methanol extract of Evolvulus nummularius.
TL;DR: The results from the study show that methanol extract of E.nummularius has antibacterial activity and the antioxidant activity may be attributed to the presence of tannins, flavonoids and triterpenoids in the methanal extract.
Abstract: Objective : To evaluate the antibacterial and antioxidant activity of methanol extract of Evolvulus nummularius (L) L. Materials and Methods : Disc diffusion and broth serial dilution tests were used to determine the antibacterial activity of the methanol extract against two Gram-positive bacterial strains (Bacillus subtilus NCIM 2718, Staphylococcus aureus ATCC 25923) and three Gram-negative bacterial strains (Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 70063 and Escherichia coli ATCC 25922). The methanol extract was subjected to preliminary phytochemical analysis. Free radical scavenging activity of the methanol extract at different concentrations was determined with 2, 2-diphenyl-1picrylhydrazyl (DPPH). Results : The susceptible organisms to the methanol extract were Escherichia coli (MIC=12.50 mg/ml) and Bacillus subtilus (MIC=3.125 mg/ml) and the most resistant strains were Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The methanol extracts exhibited radical scavenging activity with IC50 of 350 μg/ml. Conclusion : The results from the study show that methanol extract of E.nummularius has antibacterial activity. The antioxidant activity may be attributed to the presence of tannins, flavonoids and triterpenoids in the methanol extract. The antibacterial and antioxidant activity exhibited by the methanol extract can be corroborated to the usage of this plant in Indian folk medicine.
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TL;DR: In this article, a weed monitoring and survey was conducted to detect appearance of new weed species, which included Galium spurium Linn. (family Rubiaceae), Geranium nepalense Sweet (family Geraniaceae), Lamium amplexicaule Linn (family Lamiaceae), Evolvulus nummularius (family Convolvulaceae), Euphorbia thymifolia Linn.(family Euphorbiaceae), Oenothera laciniata Hill (family Onagraceae), Soliva anthemifolia (J
Abstract: Weed monitoring and survey was conducted to detect appearance of new weed species. Ten new weed species namely Galium spurium Linn. (family Rubiaceae), Geranium nepalense Sweet (family Geraniaceae), Lamium amplexicaule Linn. (family Lamiaceae), Evolvulus nummularius (family Convolvulaceae), Euphorbia thymifolia Linn.(family Euphorbiaceae), Oenothera laciniata Hill (family Onagraceae), Soliva anthemifolia (Juss.) R. Brown (family Asteraceae), Verbesina encelioides (Cav.) Benth. and Hook. f. ex A. Gray (family Asteraceae), Nicotiana plumbaginifolia Viv. (family Solanaceae) and Portulaca oleracea L. (family Portulacaceae) have been recorded in the last decade from different places of Punjab State. Out of these, weed species namely, O. laciniata, L. amplexicaule, S. anthemifolia and G. nepalense were mainly observed inhabiting the crop fields. Three weed species namely, G. spurium, N. plumbaginifolia and V. encelioides were observed growing along roadsides, wastelands, canal banks and uncultivated areas. P. oleracea and E. thymifolia were observed growing on bunds of agricultural fields. E. nummularius is a serious weed of lawns and landscapes. These weeds have now domesticated in the studied ecological niche and may potentially harm the biodiversity of those areas.
11 citations
01 Jan 2012
TL;DR: All the three compounds showed excellent antifungal activity against all the fungi tested and were able to inhibit their growth at the concentration of 5μg/ml.
Abstract: Development of microbial resistance to various existing antimicrobial agents has become a serious public health concern and the search for new classes of antimicrobial agents is a challenging task. The focus is on naturally occurring substances and their derivatives. Flavonoids are a group of naturally occurring agents and have been shown to possess good antimicrobial activity. The present study examines the antibacterial, antifungal and antimycobacterial activities of three synthetic dimethoxy flavones -3,6-dimethoxy flavone, 6,2'-dimethoxy flavone and 6,3'-dimethoxy flavone. All the three compounds showed a similar pattern of antibacterial activity. They were most active against E.coli, K.pnemoniae, S.typhi, S.paratyphi B, Citrobacter and S.marscesens and were able to inhibit their growth even at concentrations of 160μg/ml. E.faecalis and Micrococci were inhibited at concentration of 240μg/ml whereas S.aureus could be inhibited at concentration of 320μg/ml only. All the three compounds showed excellent antifungal activity against all the fungi tested and were able to inhibit their growth at the concentration of 5μg/ml.The three compounds showed different activity pattern against M.tuberculosis. While 3,6-dimethoxy flavone and 6,2'-dimethoxy flavone showed MIC values of greater than 100 μg/ml, 6,3'-dimethoxy flavone could inhibit the growth of the organism at 100μg/ml.
7 citations
TL;DR: In this article, the authors identified three novel antioxidants, subamolide C (1), subamlide E (2), and isokotomolide A (3), from the constitutes of Cinnamomum subavenium and C. kotoense.
Abstract: In this study, we identified three novel antioxidants, subamolide C (1), subamolide E (2) and isokotomolide A (3) from the constitutes of Cinnamomum subavenium andCinnamomum kotoense. The radical scavenging properties and two more antioxidant activities of the three compounds were examined. All compounds presented effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging capabilities compared with vitamin C; the metal chelating power evaluated to ethylene diamine tetra-acetic acid (EDTA) and the reducing power judged from 3-tert-butyl-4- hydroxyanisole (BHA). The data proposed that the bio-components from C. subavenium and C. kotoense act as natural antioxidants and play a potential role in cancer prevention. Meanwhile, the pure constituents will be used as leading compounds in the developments of useful cosmetic ingredients or future human disease therapies.
Key words: Cinnamomum subavenium, Cinnamomum kotoense, subamolide C, subamolide E, isokotomolide A, antioxidant.
7 citations
Cites background from "Antibacterial and antioxidant activ..."
...…antioxidants from plant species which reduce oxidative stress from intrinsic and external sources have huge applications and functions in human health care (Gülçin et al., 2001; Sundararajan et al., 2006; Furiga et al., 2008; Almajano et al., 2008; Pavithra et al., 2009; Wang et al., 2010)....
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TL;DR: The Brazil is considered a megadiverse country, with a diversified flora, present in their different biomes, with characteristics especially typical, with plants species with different popular use, major of medicinal utilization.
Abstract: The Brazil is considered a megadiverse country, with a diversified flora, present in their different biomes, with characteristics especially typical, with plants species with different popular use, major of medicinal utilization. Known the plants species used for the people is important, for very species same not were investigated of scientific form, verifying their potentialities medicinal and therapeutically. The research presented 70 species, distributed in 58 genera and 28 botanical families. The work present for all species with scientific name, family, Common name, part used, use form and Therapeutic indication. Plates with imagens of any plants species medicinal are presents.
6 citations
Cites background from "Antibacterial and antioxidant activ..."
...…(L.) L.: Family: Convolvulaceae; Common name : Aakhukarni, Muusaakarni and Chhinipatra; Part used: Whole plant; Form of use: metabolic extracts; Therapeutic indication: Remedy for hysteria, to cure burns, cuts, wounds and scorpion bites, anti-helminthine, wound healing (PAVITHRA et al., 2009)....
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...: Family: Convolvulaceae; Common name : Aakhukarni, Muusaakarni and Chhinipatra; Part used: Whole plant; Form of use: metabolic extracts; Therapeutic indication: Remedy for hysteria, to cure burns, cuts, wounds and scorpion bites, anti-helminthine, wound healing (PAVITHRA et al., 2009)....
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TL;DR: In this paper, the phytochemical constituents and several biological activities of crude methanol extracts of a native plant, Murdannia nudiflora, an exotic plant, Tradescantia pallida, and a common spice, Piper nigrum, as well as their organic soluble fractions were evaluated.
Abstract: The study was designed to determine the phytochemical constituents and to evaluate several biological activities of crude methanol extracts of a native plant, Murdannia nudiflora, an exotic plant, Tradescantia pallida, and a common spice, Piper nigrum, as well as their organic soluble fractions. Preliminary phytochemical screening confirmed the presence of several secondary metabolites.The petroleum ether extract of P. nigrum exhibited the highest degree of cytotoxicity (LC50=3.524 μg/ml) whereas that of M. nudiflora and T. pallida were quite low. Antioxidant studies of the plant extracts yielded prominent results where the free radical scavenging potential of M. Nudiflora and T. Pallida were found to be 95.76 ± 1.34 mg/100g and 97.15 ± 0.96mg/100g of AAE, respectively, whereas, IC50 values of M. nudiflora and T. pallida were 1.51x10-10μg/ml and 4.209x10-13μg/ml, respectively, justifying the presence of high concentration of flavonoids. In addition, the analgesic studies of M. nudiflora and T. Pallida revealed significant inhibition of writhing upon induction of pain by acetic acid (p<0.001, p<0.05, respectively). The chloroform extract of P.nigrum showed 61.27% inhibition of hypotonic solution induced and 96.61% inhibition of heat induced haemolysis, while the methanolic extract of the plant produced 30.74% of clot lysis confirming a moderate thrombolytic activity. Thus, the present study strives to provide a scientific basis of the traditional uses of the medicinal plants.
5 citations
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TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Abstract: METHODS for measuring antioxidants and appraising antioxidant activity appear to be of two general types. If the chemical nature of the antioxidant is known, one may strive for a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups. Alternatively one may observe the inhibition of some natural oxidative process such as the β-oxidation of fats, as a function of the added antioxidant.
10,611 citations
"Antibacterial and antioxidant activ..." refers methods in this paper
...Antioxidant assay 1,1 diphenyl-2-picryl hydrazy assay The free radical scavenging activity of the methanol extract was measured with stable 1,1 diphenyl-2-picryl hydrazyl radical (DPPH) spectrophotometrically.[10] 0....
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TL;DR: The current status of botanical screening efforts, as well as in vivo studies of their effectiveness and toxicity, are summarized and the structure and antimicrobial properties of phytochemicals are addressed.
Abstract: The use of and search for drugs and dietary supplements derived from plants have accelerated in recent years. Ethnopharmacologists, botanists, microbiologists, and natural-products chemists are combing the Earth for phytochemicals and “leads” which could be developed for treatment of infectious diseases. While 25 to 50% of current pharmaceuticals are derived from plants, none are used as antimicrobials. Traditional healers have long used plants to prevent or cure infectious conditions; Western medicine is trying to duplicate their successes. Plants are rich in a wide variety of secondary metabolites, such as tannins, terpenoids, alkaloids, and flavonoids, which have been found in vitro to have antimicrobial properties. This review attempts to summarize the current status of botanical screening efforts, as well as in vivo studies of their effectiveness and toxicity. The structure and antimicrobial properties of phytochemicals are also addressed. Since many of these compounds are currently available as unregulated botanical preparations and their use by the public is increasing rapidly, clinicians need to consider the consequences of patients self-medicating with these preparations.
7,486 citations
"Antibacterial and antioxidant activ..." refers background in this paper
...Tannins are known for their astringent property and antimicrobial activity.[13] It is explained that in the wound healing process, the tannins bind to proteins of exposed tissues, thus precipitating the proteins, and forms antiseptic protective coat enabling the regeneration of new tissues to take place....
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Journal Article•
TL;DR: The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity and its application in antioxidant research is described.
Abstract: Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity
2,519 citations
"Antibacterial and antioxidant activ..." refers background in this paper
...The effect of antioxidants molecules on DPPH is due to their hydrogen donating ability.[11] The study shows that the extract...
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TL;DR: The effect of flavonoids on eicosanoid and nitric oxide generating enzymes and the effect on expression of proinflammatory genes are summarized and a potential for new anti-inflammatory agents are discussed.
876 citations
"Antibacterial and antioxidant activ..." refers background in this paper
...[17] In a recent review article, cellular mechanisms for anti-inflammatory activity of flavonoids have been explained.[18] Flavonoids possess antioxidative, radical scavenging activities and regulate cellular activities of the inflammationrelated cells: Mast cells, macrophages, lymphocytes, and neutrophils....
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...Thus, the inhibition of these enzymes by flavonoids is definitely one of the important cellular mechanisms of antiinflammation.[18] Triterpenoids also contain antiinflammatory, anticancer and antioxidant activities....
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01 Jan 1991
TL;DR: A Dictionary of Indian Folk Medicine And Ethnobotany (free) Review of Literature 28 as mentioned in this paper, in relation to health and livelihood security in district Bastar of Chhattisgarh State.
Abstract: DICTIONARY OF INDIAN FOLK MEDICINE AND ETHNOBOTANY Dictionary Of Indian Folk Medicine And Ethnobotany (FREE) Review of Literature 28 Ethnobotany in relation to health and livelihood security in district Bastar of Chhattisgarh State. medicine from Bangalore. Sat, 20 Apr 2019 11:05:00 GMT Vaccinium vitis-idaea Wikipedia Sapindus Wikipedia Sapindus is a genus of about five to twelve species of shrubs and small trees in the Lychee family, Sapindaceae, native to warm temperate to tropical regions in both the Old World and New World. Botany Plant-names ? Sinhala Plant names ?????? ??? ... Sri Lanka plant names in Sinhala, tamil, botany, ethnobotany, etymology, computer science sinhala glossary links to place names, old and new maps, philological, historical and sociological notes, links to news and events Medicinal Plants of the Russian Pharmacopoeia; their ... Ethnopharmacological relevance. Due to the location of Russia between West and East, Russian phytotherapy has accumulated and adopted approaches that originated in European and Asian traditional medicine. Who's Who of Harlans Harlan FamilyWho's Who The Harlan Connection with Mason & Dixon. Charles Mason ARS (1728-1786) was an astronomer from Gloucestershire who worked for the Astronomer Royal at Greenwich, near London. ORGANIC AND PERMACULTURE GARDENING Great Dreams The Sustainable Farming Connection. Permaculture The Earth. International Plant Genetic Resources Institute IPGRI Regional Office for Asia, the Pacific and Oceania Discovery and resupply of pharmacologically active plant ...
868 citations
"Antibacterial and antioxidant activ..." refers background in this paper
...In Indian traditional folk medicine, the whole plant is used as a medicine for hysteria, to cure burns, cuts, wounds and scropion stings.[2] In Nepal, the paste of the plant is used to treat scabies....
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