Antibody Responses to SARS-CoV-2 Following an Outbreak Among Marine Recruits With Asymptomatic or Mild Infection.
Irene Ramos,Carl Goforth,Alessandra Soares-Schanoski,Dawn L. Weir,Emily C Samuels,Shreshta Phogat,Michelle Meyer,Kai Huang,Colette Pietzsch,Yongchao Ge,Brian L. Pike,James Regeimbal,Mark P. Simons,Michael Termini,Sindhu Vangeti,Nada Marjanovic,Stephen E. Lizewski,Rhonda A. Lizewski,Mary Catherine George,Venugopalan D. Nair,Gregory R. Smith,Weiguang Mao,Maria Chikina,Christopher C. Broder,Eric D Laing,Alexander Bukreyev,Stuart C. Sealfon,Andrew G. Letizia +27 more
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In this article, the authors investigated serological responses following a SARS-CoV-2 outbreak in spring 2020 on a US Marine recruit training base, where 147 participants were isolated during an outbreak of respiratory illness were enrolled in this study, with visits approximately 6 and 10 weeks post-outbreak (PO).Abstract:
We investigated serological responses following a SARS-CoV-2 outbreak in spring 2020 on a US Marine recruit training base. 147 participants that were isolated during an outbreak of respiratory illness were enrolled in this study, with visits approximately 6 and 10 weeks post-outbreak (PO). This cohort is comprised of young healthy adults, ages 18-26, with a high rate of asymptomatic infection or mild symptoms, and therefore differs from previously reported longitudinal studies on humoral responses to SARS-CoV-2, which often focus on more diverse age populations and worse clinical presentation. 80.9% (119/147) of the participants presented with circulating IgG antibodies against SARS-CoV-2 spike (S) receptor-binding domain (RBD) at 6 weeks PO, of whom 97.3% (111/114) remained positive, with significantly decreased levels, at 10 weeks PO. Neutralizing activity was detected in all sera from SARS-CoV-2 IgG positive participants tested (n=38) at 6 and 10 weeks PO, without significant loss between time points. IgG and IgA antibodies against SARS-CoV-2 RBD, S1, S2, and the nucleocapsid (N) protein, as well neutralization activity, were generally comparable between those participants that had asymptomatic infection or mild disease. A multiplex assay including S proteins from SARS-CoV-2 and related zoonotic and human endemic betacoronaviruses revealed a positive correlation for polyclonal cross-reactivity to S after SARS-CoV-2 infection. Overall, young adults that experienced asymptomatic or mild SARS-CoV-2 infection developed comparable humoral responses, with no decrease in neutralizing activity at least up to 10 weeks after infection.read more
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Asymptomatic SARS-CoV-2 Infection Is Associated With Higher Levels of Serum IL-17C, Matrix Metalloproteinase 10 and Fibroblast Growth Factors Than Mild Symptomatic COVID-19
Alessandra Soares Schanoski,Natalie Sauerwald,Carl Goforth,Sivakumar Periasamy,Dawn L. Weir,Stephen E. Lizewski,Rhonda A. Lizewski,Yongchao Ge,Natalia Kuzmina,Venugopalan D. Nair,Sindhu Vangeti,Nada Marjanovic,Antonio Cappuccio,Wang Cheng,Sagie Mofsowitz,C. Miller,Xuechen Yu,Mary-Catherine George,Elena Zaslavsky,Alexander Bukreyev,Olga G. Troyanskaya,Stuart C. Sealfon,Andrew G. Letizia,I Ramos +23 more
TL;DR: A serum proteomic signature is found that differentiates asymptomatic and mild symptomatic infections in young adults, including potential targets for developing new therapies and prognostic tests.
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Blood Transcriptomes of Anti-SARS-CoV-2 Antibody-Positive Healthy Individuals Who Experienced Asymptomatic Versus Clinical Infection.
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Memory B-Cell Development After Asymptomatic or Mild Symptomatic SARS-CoV-2 Infection
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TL;DR: Among young adults, asymptomatic SARS-CoV-2 infection induced antibody and memory B-cell responses comparable to mild symptomatic infection.
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SARS-CoV-2 Outbreak Dynamics in an Isolated US Military Recruit Training Center With Rigorous Prevention Measures
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A methylation clock model of mild SARS‐CoV‐2 infection provides insight into immune dysregulation
Weiguang Mao,C. Miller,Venugopalan D. Nair,Yongchao Ge,Mary Anne S. Amper,Antonio Cappuccio,Mary-Catherine George,Carl Goforth,Kristen Guevara,Nada Marjanovic,German Nudelman,Hanna Pincas,Irene Ramos,Rachel Sealfon,Alessandra Soares Schanoski,Sindhu Vangeti,Mital Vasoya,Dawn L. Weir,Elena Zaslavsky,Seunghee Kim-Schulze,Sacha Gnjatic,Miriam Merad,Andrew G. Letizia,Olga G. Troyanskaya,Stuart C. Sealfon,Maria Chikina +25 more
TL;DR: In this paper , the temporal trajectory of blood epigenetic remodeling in 133 participants in a prospective study of young adults before, during, and after asymptomatic and mildly symptomatic SARS-CoV-2 infection was characterized.
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