Antihepatotoxic activity of debelalactone, a new oxirano-furanocoumarin from Phyllanthus debilis.

doi:10.1080/10286020802621864

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Antihepatotoxic activity of debelalactone, a new oxirano-furanocoumarin from Phyllanthus debilis.

Journal Article•DOI•

Antihepatotoxic activity of debelalactone, a new oxirano-furanocoumarin from Phyllanthus debilis.

Bahar Ahmed¹, Shamshir Khan¹, Amita Verma¹, Habibullah¹•Institutions (1)

Hamdard University¹

27 Jul 2009-Journal of Asian Natural Products Research (Taylor & Francis)-Vol. 11, Iss: 8, pp 687-692

TL;DR: Debelalactone exhibited a significant antihepatotoxic activity by reducing the elevated levels of serum enzymes such as serum glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP) and increasing the total protein levels against CCl4-induced toxicity in Wistar rats.

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About: This article is published in Journal of Asian Natural Products Research.The article was published on 2009-07-27. It has received 14 citations till now. The article focuses on the topics: Transaminase.

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Journal Article•DOI•

Microanalysis in Medical Biochemistry

[...]

MG Rinsler

01 Feb 1983-Journal of Clinical Pathology

TL;DR: In the current rush to use antibodies with tissue sections, much work of poor quality is being carried out, and this volume should be a considerable help to those in difficulty.

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Abstract: biologists, and biologists, with a strong contribution from Oxford. The first chapter is a comprehensive review (by Brandtzaeg) of tissue preparation methods and particularly fixation; and each of the subsequent chapters is devoted to one technique. Those covered include double-label immunofluorescence (Pryzwansky); the unlabelled antibody peroxidase-antiperoxidase (PAP) method (Burns); the protein A-gold technique (Roth); double immunoenzymatic labelling (Mason and Woolston); and the use of proteolytic enzymes (Finley and Petrusz). Each chapter has a wide range of relevant references, and methods suitable for light and electron microscopy are discussed. Although much of the text consists of methodological detail, it is still very readable. In the current rush to use antibodies with tissue sections, much work of poor quality is being carried out, and this volume should be a considerable help to those in difficulty. Even the experienced investigator will learn a great deal from it. It is a wellproduced book, with good illustrations, which fills a gap in the literature. It is highly recommended.

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109 citations

Journal Article•DOI•

The Genus Phyllanthus: An Ethnopharmacological, Phytochemical, and Pharmacological Review.

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Xin Mao¹, Ling Fang Wu¹, Hong Ling Guo², Wen Jing Chen¹, Ya Ping Cui¹, Qi Qi¹, Shi Li¹, Wen Yi Liang¹, Guang Hui Yang¹, Yan Yan Shao¹, Dan Zhu¹, Gai Mei She¹, Yun You¹, Lan Zhen Zhang¹ - Show less +10 more•Institutions (2)

Beijing University of Chinese Medicine¹, Chinese Academy of Sciences²

20 Apr 2016-Evidence-based Complementary and Alternative Medicine

TL;DR: The ethnopharmacological, phytochemical, and pharmacological studies of Phyllanthus over the past few decades are discussed, including the researches of their remarkable antiviral, antioxidant, antidiabetic, and anticancer activities.

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Abstract: The plants of the genus Phyllanthus (Euphorbiaceae) have been used as traditional medicinal materials for a long time in China, India, Brazil, and the Southeast Asian countries. They can be used for the treatment of digestive disease, jaundice, and renal calculus. This review discusses the ethnopharmacological, phytochemical, and pharmacological studies of Phyllanthus over the past few decades. More than 510 compounds have been isolated, the majority of which are lignins, triterpenoids, flavonoids, and tannins. The researches of their remarkable antiviral, antioxidant, antidiabetic, and anticancer activities have become hot topics. More pharmacological screenings and phytochemical investigations are required to support the traditional uses and develop leading compounds.

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91 citations

Additional excerpts

...Number Compounds Species Pharmacologicaleffects References 241 Furosin P. muellerianus Wound healing [169] 241 Furosin P. debilis Antioxidant [195] 242 Geraniin P. emblica Antioxidant andantitumor [111, 185, 201] 242 Geraniin P. niruri Antiviral [177] 242 Geraniin P. urinaria Immunomodulatory, antioxidant, and hypotensive [41, 163] 242 Geraniin P. virgatus Antiviral [94, 143] 242 Geraniin P. amarus Hepatoprotection, radioprotective, and anti-HIV [179–181, 194] 242 Geraniin P. myrtifolius [186] 242 Geraniin P. sellowianus Antihyperalgesic [200] 242 Geraniin P. muellerianus Wound healing andantimalarial [169, 202] 242 Geraniin P. debilis Antioxidant [195] 242 Geraniin P. matsumurae [196] 242 Geraniin P. wightianus [89] 242 Geraniin P. ussuriensis [197] 242 Geraniin P. caroliniensis [203] 243 Geraniinic acid B P. amarus [182] 244 Hippomanin A P. urinaria Antiviral [204] 245 Isocorilagin P. emblica Antioxidant andantitumor [185, 201, 205] 245 Isocorilagin P. niruri Cholinesteraseinhibition [206, 207] 246 Isomallotusinin P. emblica Antioxidant [185] 247 Isostrictinin P. emblica [208] 247 Isostrictinin P. urinaria [209] 248 Mallonin P. emblica [184] 249 Mallotusinin P. emblica Antioxidant [210] 249 Mallotusinin P. myrtifolius [186] 250 Neochebulagic acid P. emblica [184] 251 Phyllanemblinin A P. emblica [184] 251 Phyllanemblinin A P. flexuosus [211] 252 Phyllanemblinin B P. emblica [184] 252 Phyllanemblinin B P. flexuosus [211] 253 Phyllanemblinin C P. emblica [184] 253 Phyllanemblinin C P. flexuosus [211] 254 Phyllanemblinin D P. emblica [184] 254 Phyllanemblinin D P. flexuosus [211] 255 Phyllanemblinin E P. emblica [184] 255 Phyllanemblinin E P. flexuosus [211] 256 Phyllanemblinin F P. emblica [184] 257 Phyllanthunin P. emblica [212] 258 PhyllanthusiinC P. myrtifolius [186] 259 PhyllanthusiinD P. niruri [176] Table 2: Continued....
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...Number Compounds Species Pharmacologicaleffects References 162 Phyllanthusmin B P. poilanei [123] 162 Phyllanthusmin B P. oligospermus [150] 163 Phyllanthusmin C P. reticulatus [13] 163 Phyllanthusmin C P. flexuosus [67] 163 Phyllanthusmin C P. poilanei Antitumor [123] 163 Phyllanthusmin C P. oligospermus [150] 164 Phyllanthusmin D P. poilanei [123] 165 Phyllanthusmin E P. poilanei [123] 166 Phyllanthusmin D P. flexuosus [67] 167 Phyllanthusmin E P. flexuosus [67] 168 Phyllanthusmin F P. flexuosus [67] 169 Phyltetralin P. niruri [128] 169 Phyltetralin P. urinaria Anti-inflammatory [125, 151] 169 Phyltetralin P. virgatus [131] 169 Phyltetralin P. amarus Anti-inflammatory [145] 170 Piscatorin P. piscatorum Antitumor [139] 171 Reticulatuside A P. reticulatus [13] 172 Reticulatuside B P. reticulatus [13] 173 Retrojusticidin B P. myrtifolius Anti-HIV [137, 152] 174 Seco-4-hydroxylintetralin P. niruri [153] 175 Taxodiifoloside P. taxodiifolius Antitumor [124] 176 Urinatetralin P. niruri [154] 176 Urinatetralin P. urinaria [125] 177 2,3-Desmethoxy seco-isolintetralin P. niruri [155] 178 2,3-Desmethoxy seco-isolintetralin diacetate P. niruri [155] 179 4-(3,4-Dimethoxy-phenyl)-1-(7-methoxy-benzo[1,3]dioxol-5-yl)-2,3-bis-methoxymethyl-butan-1-ol P. amarus [132] 180 5-Demethoxy niranthin P. urinaria [125] 180 5-Demethoxy niranthin P. amarus [132] 181 7-Hydroxy-3,4,5,9,9-pentamethoxy-3,4-methylene dioxy lignan P. urinaria Antitumor [156] 182 Demethylenedioxyniranthin P. niruri [155] 183 Dihydrocubebin P. niruri [155] 183 Dihydrocubebin P. urinaria [73] 184 Hydroxyniranthin P. niruri [153] 185 Linnanthin P. niruri [155] 186 Niranthin P. niruri [157] 186 Niranthin P. urinaria [125] 186 Niranthin P. virgatus Antiviral [131, 143] 186 Niranthin P. amarus Anti-inflammatory, antiparasitic, antihyperalgesic, and antitumor [132, 144, 158, 159] 187 Nirphyllin P. niruri [160] 188 Phyllanthin P. niruri Hepatoprotection, hypotensive, and antihyperuricemic [127, 157, 161, 162] 188 Phyllanthin P. urinaria Immunomodulatoryand hypotensive [125, 130, 163] Table 2: Continued....
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...Number Compounds Species Pharmacologicaleffects References 103 Phyllanthostatin 2 P. veuminatus Antitumor [117] 104 Phyllanthostatin 3 P. acuminatus Antitumor [117] 104 Phyllanthostatin 3 P. veuminatus Antitumor [117] 105 Phyllanthostatin 6 P. acuminatus Antitumor [104] 106 Phyllanthusol A P. acidus Antitumor [46] 107 Phyllanthusol B P. acidus Antitumor [46] 108 𝛽-Caryophyllene P. emblica [113] 109 𝛽-Bourbonene P. emblica [113] 110 19-Hydroxyspruceanol 19-O-𝛽-D-glucopyranoside P. reticulatus [118] 111 Cleistanthol P. urinaria [73] 111 Cleistanthol P. reticulatus [13] 111 Cleistanthol P. flexuosus Antitumor [119] 111 Cleistanthol P. oxyphyllus [90] 112 Ent-3𝛽-Hydroxykaur-l6-ene P. flexuosus [80] 113 Orthosiphol G P. niruri [120] 114 Orthosiphol I P. niruri [120] 115 Phyllanflexoid A P. flexuosus Antitumor [119] 116 Phyllanflexoid B P. flexuosus Antitumor [119] 117 Phyllanflexoid C P. flexuosus [119] 118 Phyllanterpenyl ester P. fraternus [121] 119 Spruceanol P. urinaria [73] 119 Spruceanol P. reticulatus [13] 119 Spruceanol P. oxyphyllus [90] 119 Spruceanol P. songboiensis [65] 120 trans-Phytol P. niruri [122] 121 (3S,5R,6S,9R)-Megastigmane-3,9-diol 3-O-𝛼-L-arabinofuranosyl-(1→6)-𝛽-D-glucopyranoside P. reticulatus [13] 122 (6R)-Menthiafolic acid P. urinaria [73] 123 7-Megastigmen-3-ol-9-one 3-O-𝛼-L-arabinofuranosyl-(1→6)-𝛽-D-glucopyranoside P. reticulatus [13] 124 Turpenionoside A P. reticulatus [118] 125 Turpenionoside B P. reticulatus [118] 126 7-O-[(2,3,4-Tri-O-acetyl)-𝛼-L-arabinopyranosyl]diphyllin P. poilanei Antitumor [123] 127 Arabelline P. flexuosus [67] 128 Acutissimalignans A P. songboiensis [65] 128 Acutissimalignans A P. acutissima [66] 129 Cleistanthin A P. taxodiifolius Antitumor [96, 124] 130 Cleistanthin A acetate P. taxodiifolius Antitumor [96, 124] 131 Cleistanthin A Me ether P. taxodiifolius Antitumor [96, 124] 132 Cleistanthin B P. poilanei [123] 133 Cleistanthoside A P. taxodiifolius [96] 134 Cleistanthoside A tetraacetate P. taxodiifolius Antitumor [96, 124] 135 Dextrobursehernin P. urinaria [125] 136 Diphyllin P. poilanei [123] 136 Diphyllin P. polyphyllus Anti-inflammatory [126] 137 Hypophyllanthin P. niruri Hepatoprotection andhypotensive [127–129] Table 2: Continued....
[...]
...Number Compounds Species Pharmacologicaleffects References 217 Caffeic acid P. simplex [170] 218 Cinnamic acid P. emblica Antioxidant [171] 219 Coniferyl aldehyde P. emblica [114] 220 Evofolin B P. urinaria [73] 221 Ferulic acid P. urinaria [172] 221 Ferulic acid P. simplex [170] 222 Methyl caffeate P. emblica [114] 223 Phyllanthuoside A P. cochinchinensis Antitumor [149] 224 Phyllanthuoside B P. cochinchinensis [149] 225 Debelalactone P. debilis Hepatoprotection [173] 226 Isofraxidin P. sellowianus [174] 227 Scopoletin P. sellowianus [174] 228 1,2,4,6-Tetra-O-galloyl-𝛽-D-glucose P. emblica Antiviral [175] 228 1,2,4,6-Tetra-O-galloyl-𝛽-D-glucose P. niruri Antiviral [176, 177] 229 1,3,4,6-Tetra-O-galloyl-𝛽-D-glucose P. virgatus [94] 230 1,4,6-Tri-O-galloyl-𝛽-D-glucose P. virgatus [94] 231 1,6-Di-O-galloyl-𝛽-D-glucose P. virgatus [94] 232 1,2-Di-O-galloyl-3,6-(R)-hexa-hydroxydiphenoyl-𝛽-D-glucose P. niruri [176] 233 Amariin P. amarus Hepatoprotection, radioprotective, and antioxidant [178–181] 234 Amariinic acid P. amarus [182] 235 Amarulone P. amarus [183] 236 Carpinusnin P. emblica [184] 237 Chebulagic acid P. emblica Antioxidant andantitumor [111, 184, 185] 237 Chebulagic acid P. myrtifolius [186] 238 Chebulanin P. emblica Antioxidant [184, 185] 239 Corilagin P. emblica Antioxidant andantitumor [111, 184, 187] 239 Corilagin P. niruri Antihyperalgesic andanti-inflammatory [6, 176, 188] 239 Corilagin P. urinaria Antiviral andantiplatelet [189–191] 239 Corilagin P. reticulatus [192] 239 Corilagin P. virgatus [94] 239 Corilagin P. amarus Antidiabetic, radioprotective, and anti-HIV [179, 181, 193, 194] 239 Corilagin P. myrtifolius [186] 239 Corilagin P. muellerianus [169] 239 Corilagin P. debilis Antioxidant [195] 239 Corilagin P. matsumurae [196] 239 Corilagin P. wightianus [89] 239 Corilagin P. ussuriensis Antioxidant [197, 198] 240 Excoecarianin P. urinaria Antiviral [199] 241 Furosin P. emblica Antioxidant [184, 187] 241 Furosin P. virgatus [94] 241 Furosin P. sellowianus Antihyperalgesic [200] Table 2: Continued....
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...debilis Hepatoprotection [173] 226 Isofraxidin P....
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Journal Article•DOI•

An overview of important ethnomedicinal herbs of Phyllanthus species: present status and future prospects.

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Bharti Sarin¹, Nidhi Verma², Juan Pedro Martín³, Aparajita Mohanty¹•Institutions (3)

University of Delhi¹, Indian Agricultural Research Institute², Technical University of Madrid³

03 Feb 2014-The Scientific World Journal

TL;DR: This review is an attempt to present an overview of the existing studies on pharmacognostics, phytochemistry, species identification, and genetic diversity of Phyllanthus herbs to highlight areas where further research is needed and draw attention towards extending similar studies in underutilized but potentially important herbs.

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Abstract: The genus Phyllanthus consists of more than 1000 species, of which many are used as traditional medicines. The plant extracts have been used since ancient times, for treating hypertension, diabetes, hepatic, urinary, and sexual disorders, and other common ailments. Modern day scientific investigations have now confirmed pharmacognostic properties of Phyllanthus herbs. The phytochemicals attributing these medicinal properties have been identified in many of the Phyllanthus herbs. The morphologically similar herbs of Phyllanthus grow together and admixture of species during collection for manufacture of herbal medicines is quite common. Hence, along with pharmacognostic and phytochemical studies, appropriate protocols for correct identification of species are also important. As the use of these herbs as green medicines is becoming more popular, it is imperative to assess its genetic diversity and phylogenetic relatedness for future conservation strategies. This review is an attempt to present an overview of the existing studies on pharmacognostics, phytochemistry, species identification, and genetic diversity of Phyllanthus herbs and consequently (i) highlight areas where further research is needed and (ii) draw attention towards extending similar studies in underutilized but potentially important herbs such as P. maderaspatensis, P. kozhikodianus, P. rheedii, P. scabrifolius, and P. rotundifolius.

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74 citations

Cites background from "Antihepatotoxic activity of debelal..."

...debilis Oxirano-furanocoumarin Debelalactone Antihepatotoxic [25]...
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...This herb shows antihepatotoxic [25] and anti-inflammatory [76] properties....
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...debilis is shown to possess antihepatotoxic ability [25]....
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...debilis Oxirano-furanocoumarin Decalactone [25]...
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Journal Article•DOI•

Lead finding from Phyllanthus debelis with hepatoprotective potentials

[...]

Amita Verma¹•Institutions (1)

Sam Higginbottom Institute of Agriculture, Technology and Sciences¹

01 Jan 2012-Asian pacific Journal of Tropical Biomedicine

TL;DR: The study of antihepatotoxic activity and molecular prediction of compounds isolated from Phyllanthus debelis shows that compound V debelolactone has good drug likeness score with no violations & good bioactivity score as compared to silibinin which is potent hepatoprotective drug.

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Abstract: Objective Present communication deals with the study of antihepatotoxic activity and molecular prediction of compounds isolated from Phyllanthus debelis in order to search lead compound. Methods Five compounds from the whole plant of P. debelis were subjected to molecular properties prediction and drug-likeness by Lipinski rule of five & Molinspiration software. Results All the compounds were found in compliance with Lipinski ‘Rule of Five’ except the Milog P valve of compound No. I-IV were found above five means these compounds have less permeability across the cell membrane. The Milog P valve of Compound No V & standard compound silibinin VI were found below five, suggest that the molecules have good permeability across the cell membrane. In respect of TPSA, all the compounds were within the limit i.e. 160 A. The bioactivity score was also calculated for GPCR ligand, ion channel modulator, kinase inhibitor, nuclear receptor ligand. All the compound showed activity through enzyme inhibition. Conclusions Our study shows that compound V debelolactone has good drug likeness score with no violations & good bioactivity score as compared to silibinin which is potent hepatoprotective drug. So compound V debelolactone can be a lead compound with hepatoprotective activity from Phyllanthus debelis .

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72 citations

Cites background or result from "Antihepatotoxic activity of debelal..."

...Our previous study showed that Phyllanthus debelis extract & compound V has significant antihepatotoxic activity against CCl4 induced toxicity on wistar rats [4, 5]....
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...We have published a paper of the antihepatotoxic activity of compound V named debelolactone [5]....
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Journal Article•DOI•

Chemical Constituents of the Plants from the Genus Phyllanthus

[...]

Wei-Yan Qi¹, Lei Hua¹, Kun Gao², Kun Gao¹•Institutions (2)

Lanzhou University¹, Zhejiang University²

01 Mar 2014-Chemistry & Biodiversity

27 citations

References

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Journal Article•DOI•

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

[...]

Stanley Reitman¹, Sam Frankel¹•Institutions (1)

Jewish Hospital¹

01 Jul 1957-American Journal of Clinical Pathology

9,424 citations

Journal Article•DOI•

New Tables for Multiple Comparisons with a Control

[...]

Charles W. Dunnett

01 Sep 1964-Biometrics

TL;DR: The main purpose of the present paper is to give the exact tables for making two-sided comparisons, and a method is given for adjusting the tabulated values to cover the situation where the variance of the control mean is smaller than thevariance of the treatment means.

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Abstract: Some time ago, a multiple comparison procedure for comparing several treatments simultaneously with a control or standard treatment was introduced by the present author (Dunnett [1955]). The procedure was designed to be used either to test the significance of the differences between each of the treatments and the control with a stated value 1 - P for the joint significance level, or to set confidence limits on the true values of the treatment differences from the control with a stated value P for the joint confidence coefficient. Thus the procedure has the property of controlling the experimentwise, rather than the per-comparison, error rate associated with the comparisons, in common with the multiple comparison procedures of Tukey [unpublished] and Scheffe [1953]. In the earlier paper, tables were provided enabling up to nine treatments to be compared with a control with joint confidence coefficient either .95 or .99. Tables for both one-sided and two-sided comparisons were given but, as explained in the paper, the two-sided values were inexact for the case of more than two comparisons as a result of an approximation which had to be made in the computations. The main purpose of the present paper is to give the exact tables for making two-sided comparisons. The necessary computations were done on a General Precision LGP-30 electronic computer, by a method described in section 3 below. The tables are given here as Tables II and III; these replace Tables 2a and 2b, respectively, of the previous paper. In addition to providing the exact values, a method is given for adjusting the tabulated values to cover the situation where the variance of the control mean is smaller than the variance of the treatment means, as occurs for example when a greater number of observations is allocated to the control than to any of the test treatments. Furthermore, the number of treatments which may be simultaneously compared with a control has been extended to twenty. 482

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2,823 citations

Journal Article•DOI•

Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine

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P. R. N. Kind, E. J. King

01 Nov 1954-Journal of Clinical Pathology

TL;DR: The conditions under which enzymic hydrolysis is carried out are the same as in the King-Armstrong method, as modified by King (1951), the amino-antipyrine reagent (A.A.P.) being used to estimate the phenol liberated.

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Abstract: Plasma phosphatase was first determined by Martland (1925) and then by Kay (1930), using glycerophosphate and estimating the inorganic phosphate liberated at 370 C. at a specified pH and in a given time. The procedure has been variously modified and simplified by Jenner and Kay (1932), Bodansky (1933), Shinowara, Jones, and Reinhart (1942). Several substrates, other than glycerophosphate, have also been used, e.g., phenyl phosphate (with determination of either the liberated phenol, King and Armstrong, 1934, or phosphate, King, Abul-Fadl, and Walker, 1951), nitro-phenyl phosphate (by the colour of the liberated nitrophenol, Ohmori, 1937; King and Delory, 1939; Bessey, Lowry, and Brock, 1946), naphthyl phosphate (by determination of naphthol by a diazo method, Seligman, Chauncey, Nachlas, Manheimer, and Ravin, 1951), phenolphthalein phosphate (by the colour of alkaline phenolphthalein, Bray and King, 1943; Huggins and Talalay, 1945). Of the.se procedures, the Bodansky (glycerophosphate) and the King-Armstrong (phenyl phosphate) are perhaps the most widely used. King and Armstrong (1934) determined free phenol, liberated from phenyl phosphate by the phosphatase, by either the Folin and Ciocalteu (1927) phosphomolybdic reagent or by the diazo reaction, and preferred the former. Both procedures required the precipitation and removal of the plasma proteins subsequent to the hydrolysis of the substrate. In 1951 Grifols Lucas proposed a procedure for determination of the free phenol, which did not require removal of the proteins. This was based on the 4-amino-antipyrine reaction of Gottlieb and Marsh (1946). In this method the conditions under which enzymic hydrolysis is carried out are the same as in the King-Armstrong method, as modified by King (1951), the amino-antipyrine reagent (A.A.P.) being used to estimate the phenol liberated. 4-Amino-antipyrine reacts with certain phenolic substances in the presence of alkaline oxidizing agents to produce quinonoid substitution products. These give a red colour proportional to the phenol present, and can be determined colorimetrically. Grifols-Lucas found that A.A.P. does not react with plasma proteins, and their removal is unnecessary when phosphatases are estimated.

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1,665 citations

Book•

Drug discovery and evaluation : pharmacological assays

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H. Gerhard Vogel

15 Jan 1997

TL;DR: Thoroughly revised and expanded to two volumes, this successful reference offers an updated selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs.

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Abstract: The new edition of this successful reference offers both cutting-edge and classic pharmacological methods. Thoroughly revised and expanded to two volumes, it offers an updated selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs. Every chapter has been updated, and numerous assays have been added. Each of the more than 1,000 assays comprises a detailed protocol outlining purpose and rationale, and a critical assessment of the results and their pharmacological and clinical relevance.

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1,396 citations

Book•

Microanalysis in Medical Biochemistry

[...]

Earl J King

01 Jan 1964

TL;DR: Micro- analysis in medical biochemistry, Micro-analysis in medicalBiochemistry, and Micro- Analysis in Medical biochemistry .

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Abstract: Micro-analysis in medical biochemistry , Micro-analysis in medical biochemistry , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

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1,385 citations