DOI•
Antioxidant and Antitumor Activity of Indonesian Herbal Ingredients
14 Dec 2015-Vol. 16, Iss: 2, pp 68-74
TL;DR: The results indicated that the cell death receptor pathway was involved in the apoptosis induced by Indonesian herbal extracts, and total phenol content.
Abstract: The present study was to aimed to evaluated and compare in vitro antioxidant activities of 2 Indonesian herbal ingredients (A and B), determined total phenol content., cytotoxic and apoptosis induction activities on HL-60 cells. These data were providing some useful information for people healthy dietary and the new potential application of natural antioxidant containing food materials in functional foods and also as new cancer therapeutics promising candidates. The parameters were total antioxidant activity, amount of total cytotoxic effect on the growth of human promyelocytic leukemia cells (HL-60). Statistical comparison was perform with Student’s t-test at p<0.05. The correlation coefficient (r2) between the parameters tested was established by regression analysis. The scavenging effect of extracts herbal on DPPH radicals increased from 0.3-1.5 mg/ml, where is sample A 14.33% to 64.29% and sample B 9.09% to 57.53% was obtained. High content of total phenol compounds were in sample A (21.72 mg GAE/g), lower amounts were in sample B (17.53 mg GAE/g). Apoptosis of HL-60 cells from the morphological changes side (chromatin condensation). Chromatin condensation, a specific and distinct feature of apoptotic cells, was found in the majority of treated cells. The results indicated that the cell death receptor pathway was involved in the apoptosis induced by Indonesian herbal extracts.
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Dissertation•
04 Apr 2013
TL;DR: Oleh sebab itu di Departemen Kesehatan Republik Indonesia mengambil kebijaksanaan bahwa bahan obat alam yang sebagian besar berasal dari tumbuh-tumbuhan perlu dilakukan penelitian dan pengembangan tanaman obat.
Abstract: Penggunaan tanaman obat dengan cara-cara tradisional semakin disukai di Indonesia, karena pada umumnya kurang menimbulkan efek samping bila dibandingkan dengan obat modern. Oleh sebab itu di Departemen Kesehatan Republik Indonesia mengambil kebijaksanaan bahwa bahan obat alam yang sebagian besar berasal dari tumbuh-tumbuhan perlu dilakukan penelitian dan pengembangan tanaman obat.
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Journal Article•
TL;DR: The results, together with previous findings, suggest that curcumin-glucuronoside, dihydrocurcumin - glucuronOSide, THC-gloucesteride, and THC are major metabolites ofCurcumin in vivo.
Abstract: Curcumin, the yellow pigment in turmeric and curry, has antioxidative and anticarcinogenic activities. In this study, we investigated the pharmacokinetic properties of curcumin in mice. After i.p. administration of curcumin (0.1 g/kg) to mice, about 2.25 μg/ml of curcumin appeared in the plasma in the first 15 min. One hour after administration, the levels of curcumin in the intestines, spleen, liver, and kidneys were 177.04, 26.06, 26.90, and 7.51 μg/g, respectively. Only traces (0.41 μg/g) were observed in the brain at 1 h. To clarify the nature of the metabolites of curcumin, the plasma was analyzed by reversed-phase HPLC, and two putative conjugates were observed. Treatment of the plasma with β-glucuronidase resulted in a decrease in the concentrations of these two putative conjugates and the concomitant appearance of tetrahydrocurcumin (THC) and curcumin, respectively. To investigate the nature of these glucuronide conjugates in vivo, the plasma was analyzed by electrospray. The chemical structures of these metabolites, determined by mass spectrometry/mass spectrometry analysis, suggested that curcumin was first biotransformed to dihydrocurcumin and THC and that these compounds subsequently were converted to monoglucuronide conjugates. Because THC is one of the major metabolites of curcumin, we studied its stability at different pH values. THC was very stable in 0.1 M phosphate buffers of various pH values. Moreover, THC was more stable than curcumin in 0.1 M phosphate buffer, pH 7.2 (37°C). These results, together with previous findings, suggest that curcumin-glucuronoside, dihydrocurcumin-glucuronoside, THC-glucuronoside, and THC are major metabolites of curcumin in vivo.
885 citations
"Antioxidant and Antitumor Activity ..." refers background in this paper
...Curcumin which contained in C. xanthorrhiza and C. aeruginosa, xanthorrhizol in C. xanthorrhiza also had an antioxidant activity (Pan et al., 1999)Marmelin in A. marmelos had been reported Majalah Obat Tradisional, 16(2), 2011 72 has an antioxidant activity (Singh et al., 2000)....
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TL;DR: The antioxidant potentials of a total ethanolic extract of Hypericum perforatum (TE) and fractions were evaluated and correlated with their phenolic contents in this article, and the extracts were fully characterised by HPLC-DAD-MS-MS.
369 citations
"Antioxidant and Antitumor Activity ..." refers background in this paper
...There is an increasing interest in natural antioxidants, e.g., polyphenols, present in medical and dietary plants which might help prevent oxidative damage (Silva et al., 2005)....
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TL;DR: In this paper, the profile of flavonol-O -glycosides and xanthone-C -Glycosides was characterized in pulps from Haden, Tommy Atkins, Palmer, and Uba mango varieties by LC-ESI-MS analysis.
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"Antioxidant and Antitumor Activity ..." refers background in this paper
...The presence of phenol compounds in the human diet is associated with protective effects against some chronic-degenerative disease related to oxidative stress (Riberio et al., 2008)....
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168 citations
TL;DR: Of 30 Indonesian plant extracts tested for their human immunodeficiency virus type‐1 (HIV‐1) inhibitory activities, six were shown to be effective by assays using HIV‐1‐infected MT‐4 cells: a methanol extract of mahoni and water extracts of benalu teh, which showed a significant inhibition of HIV‐ 1 protease.
Abstract: Of 30 Indonesian plant extracts tested for their human immunodeficiency virus type-1 (HIV-1) inhibitory activities, six were shown to be effective by assays using HIV-1-infected MT-4 cells: a methanol extract of mahoni (bark of Swietenia mahagoni) and water extracts of benalu teh (stems and branches of Loranthus parasiticus), kiules (fruit of Helicteres isora), supratul (fruits of Sindora sumatrana), sambiloto (leaves of Andrographis paniculata) and temu ireng (rhizoma of Curcuma aeruginosa). Their ED50 values ranged from 4.2 to 175 μg/mL. The samples also suppressed the formation of syncytia in co-cultures of MOLT-4 and MOLT-4/HIV-1 cells. The most potent inhibitor was a methanol extract of mahoni, which also showed a significant inhibition of HIV-1 protease.
135 citations
"Antioxidant and Antitumor Activity ..." refers background in this paper
...The water extract from the rhizome of C. aeruginosa effectively inhibited on HIV-1 infected MT-4 cells (Otake et al., 1995)....
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