Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of β-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum
TL;DR: A temperature of 30°C and an initial pH of 4.7 were found to be optimal for β-1,3-glucanase production from T. harzianum in both surface culture and submerged culture processes.
About: This article is published in Enzyme and Microbial Technology.The article was published on 1995-12-01. It has received 38 citations till now. The article focuses on the topics: Trichoderma harzianum & Trichoderma reesei.
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TL;DR: Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae and a modest high level of DA and DB yields were obtained by employing the response surface methodology optimized DB production medium in a no‐baffle, stirred‐tank fermentor.
Abstract: Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae. With the addition of 0.1% (w/v) beta-alanine to the basal medium, the yields of cyclodepsipeptides DA and DB were 7.2 and 279 mg/L, respectively, which was 2-fold higher than that of control experiment. Response surface methodology (RSM) was applied to optimize the compositions of maltose, peptone, beta-alanine, and glucose used in a shaker-flask cultivation of M. anisopliae for the production of DA and DB. Estimated optimal compositions for the DA production were maltose 2.58%, peptone 0.72%, beta-alanine 0.02%, and glucose 0.55%. The predicted DA yield was 18.5 mg/L. On the other hand, the optimal compositions for DB production were maltose 2.51%, peptone 0.75%, beta-alanine 0.02%, and glucose 0.43%. A maximum DB yield of 232 mg/L was predicted. These were confirmed by cultivation experiments conducted at the optimized conditions for maximum destruxins production in a shaker-flask. Furthermore, a modest high level of DA (49 mg/L) and DB (268 mg/L) yields were obtained by employing the response surface methodology optimized DB production medium in a no-baffle, stirred-tank fermentor.
27 citations
TL;DR: In this article, the β-1,3,1,6-d -glucan type produced by B. rhodina and Trichoderma harzianum Rifai were grown on botryosphaeran.
21 citations
TL;DR: A purified enzyme with relatively good thermostability that is stable at low pH might be used in future industrial applications.
Abstract: The enzyme glucanase from Moniliophthora perniciosa was produced in liquid medium and purified from the culture supernatant. A multivariate statistical approach (Response Surface Methodology - RSM) was employed to evaluate the effect of variables, including inducer (yeast extract) and fermentation time, on secreted glucanase activities M. perniciosa detected in the culture medium. The crude enzyme present in the supernatant was purified in two steps: precipitation with ammonium sulfate (70%) and gel filtration chromatography on Sephacryl S-200. The best inducer and fermentation time for glucanase activities were 5.9 g L(-1) and 13 days, respectively. The results revealed three different isoforms (GLUI, GLUII and GLUIII) with purification factors of 4.33, 1.86 and 3.03, respectively. The partially purified enzymatic extract showed an optimum pH of 5.0 and an optimum temperature of 40°C. The enzymatic activity increased in the presence of KCl at all concentrations studied. The glucanase activity was highest in the presence of 0.2 M NaCl. The enzyme showed high thermal stability, losing only 10.20% of its specific activity after 40 minutes of incubation at 90°C. A purified enzyme with relatively good thermostability that is stable at low pH might be used in future industrial applications.
20 citations
Cites background from "Application of response surface met..."
...…been reported, but the optimum temperature of M. perniciosa was lower when compared to An Acad Bras Cienc (2011) 83 (2) most others (Nagata et al. 1990, Fontaine et al. 1997, Bara et al. 2003, Marco and Felix 2007, Li et al. 2007), and higher in relation to T. harzianum (Théodore and Panda 1995)....
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TL;DR: In comparison with glycerol and glucose, the rice powder becomes a more suitable carbon source and represents a great potential for the production of Lvs.
Abstract: Response surface methodology (RSM) was employed to study the effect of the composition of the rice-glycerol complex medium on the production of lovastatin (Lvs) by the ascomyceteMonascus ruber in mixed solid-liquid (or submerged) cultures at 25°C. Four components (rice powder, peptone, glycerol, glucose) were studied to evaluate, the approximate polynomial for all dependent variables, explaining their effects on the production of Lvs. The best composition derived from RSM regression was (in g/L) rice powder 34.4, peptone 10.8, , glucose 129, KNO3 8.0, MgSO4·7H2O 4.0 and glycerol 36.4 mL/L. With this composition, the Lvs production was 157 mg/L after 10 d of cultivation. In comparison with glycerol and glucose, the rice powder becomes a more suitable carbon source and represents a great potential for the production of Lvs.
19 citations
TL;DR: The difference in the nature of the hydrolysis products can be explained by the substrate specificities of each enzyme fraction, and the structural differences of the β-D-glucans attacked.
Abstract: β-(1→3)-Glucanases were produced by Trichoderma harzianum Rifai PAMB-86 cultivated on botryosphaeran in a bench-fermenter and optimised by the response surface method. Maximal enzyme titres occurred at 5 days, initial pH 5.5 and aeration of 1.5vvm. β-(1→3)-The β-glucanolytic enzyme complex produced by T. harzianum Rifai PAMB86 was fractionated by gel filtration into 2 fractions (F-I, F-II), and employed to produce gluco-oligosaccharides from algal paramylon ((1→3)-β-D-glucan) and lichen pustulan ((1→6)-β-D-glucan). Both enzymes attacked paramylon to the extent of ~15-20% in 30 min releasing glucose and laminaribiose as major end-products, and laminarioligosaccharides of degree of polymerization (DP) ≥3. Only F-I degraded pustulan resulting in ~2% degradation at 30 min, with glucose, gentiobiose and gentio-oligosaccharides of DP ≥4 as major products. The difference in the nature of the hydrolysis products can be explained by the substrate specificities of each enzyme fraction, and the structural differences of the β-D-glucans attacked.
16 citations
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25,389 citations
TL;DR: Advances in Enzymology and Related Subjects of Biochemistry Vol 24, No. 6, 1962 as discussed by the authors, Section 4, Section 5, Section 2, Section 3.
Abstract: Advances in Enzymology and Related Subjects of Biochemistry Vol 24 Edited by F F Nord Pp v + 572 (New York and London: Interscience Publishers, a Division of John Wiley and Sons, 1962) 120s
625 citations
TL;DR: In this article, the importance of antibiotics and extracellular enzymes during hyphal interaction is discussed, and the hyphae of the majority of Trichoderma isolates coiled around hyphiae of different test fungi.
Abstract: When grown in dual culture, hyphae of the majority of Trichoderma isolates coiled around hyphae of different test fungi. Penetration of hyphae by Trichoderma hyphae seldom occurred. The importance of antibiotics and extracellular enzymes during hyphal interaction is discussed.
579 citations
01 Jan 1976
TL;DR: In this article, a filter paper assay method and unit value is described for the measurement of enzyme saccharification action, which is simple, reproducible, and quantitative and predicts enzyme action under practical conditions.
Abstract: A filter paper assay method and unit value is described for the measurement of enzyme saccharification action. The method is simple, reproducible, and quantitative and predicts enzyme action under practical saccharification conditions. (JSR)
505 citations
01 Jan 2011
TL;DR: Isolates of T. harzianum were found to differ in the levels of hydrolytic enzymes produced when mycelium of S. rolfsii, Rhizoctonia solani, and Pythium aphanidermatum in soil was attacked, correlated with the ability of each of the Trichoderma isolates to control the respective soilborne pathogens.
494 citations