Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of β-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum
TL;DR: A temperature of 30°C and an initial pH of 4.7 were found to be optimal for β-1,3-glucanase production from T. harzianum in both surface culture and submerged culture processes.
About: This article is published in Enzyme and Microbial Technology.The article was published on 1995-12-01. It has received 38 citations till now. The article focuses on the topics: Trichoderma harzianum & Trichoderma reesei.
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TL;DR: The above kinetics and inhibition data will serve as suitable database for the development of a mathematical model for growth and its use will be able to facilitate appropriate reactor design for the removal of phosphates from industrial effluents.
15 citations
TL;DR: The results showed that the quality of C. songaricum from Qinghai Province was the best, while the quality from Gansu province was the worst, and the scientific basis for quality evaluation, especially for food safety was found.
Abstract: The present study aimed to evaluate the quality of Cynomorium songaricum Rupr. from different producing areas, which is an edible, holoparasitic, and desert plant that has been used in traditional medicine for improving immunity and kidney function and treating constipation. We optimized the extract conditions by response surface methodology (RSM) and determined the content of gallic acid, protocatechuic acid, and catechin of C. songaricum simultaneously from different producing areas by high-performance liquid chromatography (HPLC). It is the first study in which the RSM was used to optimize the extract condition of C. songaricum with multiple evaluation factors, ensuring the efficiency and accuracy of the study. The results were analyzed by principal component analysis, and they showed that the quality of C. songaricum from Qinghai Province was the best, while the quality of C. songaricum from Gansu Province was the worst. For the first time, the two ecotypes of C. songaricum, including Outside Great Wall type and Inside Great Wall type, were discovered and identified by the chemical marker protocatechuic acid. This study is the scientific basis for quality evaluation, especially for food safety.
15 citations
Cites methods from "Application of response surface met..."
...RSM has been successfully applied to model and optimize biochemical and biotechnological processes related to food systems [16, 17]....
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TL;DR: It was observed that as the dilution rate increased, the steady state biomass concentration gradually decreased and residual substrate concentration increased, indicating the applicability of well-known Monod type growth pattern.
15 citations
01 Jan 2013
TL;DR: This is first study on levansucrase production by focusing on the production of stable enzymes during process variations, such as temperature, which can promote enzyme denaturation and high yield of enzyme production by microorganism.
Abstract: Levansucrase is a microbial enzyme, classified as fructosyltransferase and responsible for levan polymerization. Levan is a polyfructan widely used in pharmaceutical, industrial and food field. Enzymatic synthesis of polymers on industrial scale requires the use of stable enzymes during process variations, such as temperature, which can promote enzyme denaturation. In addition, high yield of enzyme production by microorganism is required. Although several studies report empirical levansucrase production, there are few information about statistical optimization levansucrase production. Furthermore, most levansucrase reported are high temperature intolerant. This is first study on levansucrase production by
13 citations
Cites background from "Application of response surface met..."
...empirical study for enzyme [22, 23] and polysaccharides production optimization, such as levan [24,25] evaluating different...
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TL;DR: The developed experimental design is effective towards process optimization for NHase production from R. rhodochrous (RS-6) and the NHase yield was enhanced up to 6.22 fold by statistical optimization using RSM.
12 citations
References
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25,389 citations
TL;DR: Advances in Enzymology and Related Subjects of Biochemistry Vol 24, No. 6, 1962 as discussed by the authors, Section 4, Section 5, Section 2, Section 3.
Abstract: Advances in Enzymology and Related Subjects of Biochemistry Vol 24 Edited by F F Nord Pp v + 572 (New York and London: Interscience Publishers, a Division of John Wiley and Sons, 1962) 120s
625 citations
TL;DR: In this article, the importance of antibiotics and extracellular enzymes during hyphal interaction is discussed, and the hyphae of the majority of Trichoderma isolates coiled around hyphiae of different test fungi.
Abstract: When grown in dual culture, hyphae of the majority of Trichoderma isolates coiled around hyphae of different test fungi. Penetration of hyphae by Trichoderma hyphae seldom occurred. The importance of antibiotics and extracellular enzymes during hyphal interaction is discussed.
579 citations
01 Jan 1976
TL;DR: In this article, a filter paper assay method and unit value is described for the measurement of enzyme saccharification action, which is simple, reproducible, and quantitative and predicts enzyme action under practical conditions.
Abstract: A filter paper assay method and unit value is described for the measurement of enzyme saccharification action. The method is simple, reproducible, and quantitative and predicts enzyme action under practical saccharification conditions. (JSR)
505 citations
01 Jan 2011
TL;DR: Isolates of T. harzianum were found to differ in the levels of hydrolytic enzymes produced when mycelium of S. rolfsii, Rhizoctonia solani, and Pythium aphanidermatum in soil was attacked, correlated with the ability of each of the Trichoderma isolates to control the respective soilborne pathogens.
494 citations