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Journal ArticleDOI

Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of β-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum

01 Dec 1995-Enzyme and Microbial Technology (Elsevier)-Vol. 17, Iss: 12, pp 1043-1049
TL;DR: A temperature of 30°C and an initial pH of 4.7 were found to be optimal for β-1,3-glucanase production from T. harzianum in both surface culture and submerged culture processes.
About: This article is published in Enzyme and Microbial Technology.The article was published on 1995-12-01. It has received 38 citations till now. The article focuses on the topics: Trichoderma harzianum & Trichoderma reesei.
Citations
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Journal ArticleDOI
03 Aug 2015
TL;DR: In this paper, the authors used active microorganisms to remove lipids from wastewater and from drain pipes surfaces, and then treated the remaining lipids and solid wastes with a composition of active micro-organisms.
Abstract: Lipids constitute one of the major types of organic matter found in municipal wastewater. Many manufacturing, food processing and industrial facilities dispose of liquid waste into sewer lines. Liquid waste often contains fats, oils and grease and other organic contaminants which, over time, lead to clogs in pipes. The treatment of this problem is to clean pipes with caustic drain cleaners, mechanically rout the pipes or to replace the pipes completely. The second problem was the utilization of solid waste. JSC “Biocentras” suggest a very effective and innovative complex technology. Firstly, lipids from wastewater and from drain pipes surfaces are removing mechanically. Remained lipids and solid wastes were treated with a composition of active microorganisms. The novel composition is nonpoisonous, no corrosive, no caustic and ecologically advantageous. The invented technology ensures maximum results at the lowest cost in the shortest period of time.

2 citations


Cites methods from "Application of response surface met..."

  • ...For all procedures optimization the response surface methodology was used [16, 18, 19], which includes design of factorial experiments, development of models for the response surface estimation and model-based search of optimum point....

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Journal ArticleDOI
05 Aug 2015
TL;DR: In this article, a new improved Sorbent system (S-S) from organic sorbent material, nutrients, biosurfactant and microbial cell producing it for bioremediation of heavy oil hydrocarbons in various soil types.
Abstract: The aim was to develop a new improved Sorbent system (S-S) from organic sorbent material, nutrients, biosurfactant and microbial cell producing it for bioremediation of heavy oil hydrocarbons in various soil types. New sorbent material (S-M) was developed from paper mill short waste fibers because it requires very low energy to manufacture and the production costs of this S-M is extremely low compared to similar products in the market. S-M has good sorption characteristics for various oil products, is fully biodegradable, can be applied at sensitive environmental areas. S-M can be combined with microorganisms producing biosurfactant. The goal of investigations was to optimize S-S composition to minimize the leakage of heavy hydrocarbons to deeper soil layers or ground water. The optimal composition of S-S for all types of soil was determined: S-M 86.3-89.3%, microorganisms producing biosurfactant ≥ 107 CFU/g, biosurfactant solution – 3%, nutrients (nitrogen and phosphorous) 0.5 and 0.2%. Recommended optimal S-S moisture is 7%.

1 citations


Cites background from "Application of response surface met..."

  • ...xn*] T , at which the minimum yield of hydrocarbon leakage with the particular S-S composition was obtained, and determination of optimal point location [7, 8; 9]....

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Journal Article
TL;DR: Sao Paulo State University UNESP, IBILCE Laboratory of Biochemistry and Applied Microbiology, R. Cristovao Colombo 2265, 15054-000 Sao Jose do Rio Preto-SP.
Abstract: Sao Paulo State University UNESP, IBILCE Laboratory of Biochemistry and Applied Microbiology, R. Cristovao Colombo 2265, 15054-000 Sao Jose do Rio Preto-SP

1 citations


Cites background from "Application of response surface met..."

  • ...(24) and Theódore and Panda (25) to optimize the production of b-1,3-glucanase by some species of Trichoderma in submerged fermentation using different b-(1®3,1®6)-D-glucans as the carbon source....

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  • ...(23) and Théodore and Panda (25) in the optimization of the production of b-1,3-glucanases using Trichoderma harzianum....

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  • ...(24) and Théodore and Panda (25) in cultures of T....

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  • ...The highest b-1,3-glucanase production was obtained by Theódore and Panda (25) when Trichoderma harzianum was grown in glucose at 30 °C and at initial pH=4....

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Journal ArticleDOI
TL;DR: In this paper, the authors optimize the extraction of phenolics and flavonoids from P. graveolens by response surface methodology with particular attention on the proliferative and cytotoxic effects on human keratinocytes, as well as the antioxidant and antibacterial activities.
Abstract: Context: Pelargonium graveolens Lʼ Her is an evergreen shrub, cultivated principally for the medicinal essence and decoction in Southern Africa for the treatment of menopausal problems, tonsillitis, poor circulation, ringworm, and cervical cancer. Objective: We aimed to optimize the extraction of phenolics and flavonoids from P. graveolens by response surface methodology with particular attention on the proliferative and cytotoxic effects on human keratinocytes, as well as the antioxidant and antibacterial activities. Materials and Methods: The optimization was achieved by Box–Behnken design. Extract, metabolite yields, and minimal inhibitory concentrations (MIC) were determined by gravimetric, spectrophotometric, and microdilution methods, respectively. The antiradical potentials were evaluated using the phosphomolybdate, 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and lipid peroxidation assays. The kinetics of the lipid protective activity was studied and fitted into models. The proliferative and cytotoxic effects were evaluated using the CellTiter® Blue cell viability and lactate dehydrogenase assay. Results: The regression coefficient r2 ≥ 0.9775 indicated a close correlation between actual and predicted values of the responses. The ideal parameter for the extraction of phenolics and flavonoids by macerations was determined as an extraction time: 9.63–12.01 h, material mass: 2.78–2.62 g, agitation speed: 143.11–191.37 rpm, and solvent volume: 68.06–69.87 mL. Total antioxidant capacity and reducing power were comparable to standard gallic acid, while the antiradical activity has IC50 values of 0.18 ± 0.03–4.98 ± 0.15 mg/mL. Further, the lipid protective revealed a dose-dependent activity fitting into a pseudo-second-order kinetic model. MIC value of 1.56 mg/mL was registered against Staphylococcus aureus and Salmonella typhi compared to chloramphenicol. There was a significant (P < 0.05) increase in cell proliferation and viability when the extract was administered at concentrations of ≤50 μg/mL. However, at ≥100 μg/mL concentration, there was significant cytotoxicity in comparison to the untreated cells. Conclusion: These biological activities are confirmation of the phytomedicinal application and possible source of pharmaceutical compounds. However, administration of the decoction should take into cognizance the antiproliferative effect at doses ≥100 μg/mL as well as the potential to induce and maintain keratinocyte proliferation at low concentration with an eye on the antiproliferative effect at concentrations ≥100 μg/mL.

1 citations


Cites methods from "Application of response surface met..."

  • ...[17,18] RSM is a useful statistical method that uses a minimum of resources and quantitative data from the appropriate experimental design to determine and simultaneously solve a multivariate equation.[19] It usually uses an experimental design such as central composite or Box–Behnken Designs (BBD) to fit an empirical, full second-order polynomial model, which generally provides an adequate representation of the most continuous response surfaces over a relatively broad factor domain....

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Journal ArticleDOI
TL;DR: The generalized distance function approach is employed to obtain a suitable near optimal conditions of variables and can be considered as a 'near optimal' solution of interactive multi-response systems of intracellular esterase synthesis by Saccharomyces cerevisiae.

1 citations

References
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Journal ArticleDOI
01 Dec 1963-Nature
TL;DR: Advances in Enzymology and Related Subjects of Biochemistry Vol 24, No. 6, 1962 as discussed by the authors, Section 4, Section 5, Section 2, Section 3.
Abstract: Advances in Enzymology and Related Subjects of Biochemistry Vol 24 Edited by F F Nord Pp v + 572 (New York and London: Interscience Publishers, a Division of John Wiley and Sons, 1962) 120s

625 citations

Journal ArticleDOI
TL;DR: In this article, the importance of antibiotics and extracellular enzymes during hyphal interaction is discussed, and the hyphae of the majority of Trichoderma isolates coiled around hyphiae of different test fungi.
Abstract: When grown in dual culture, hyphae of the majority of Trichoderma isolates coiled around hyphae of different test fungi. Penetration of hyphae by Trichoderma hyphae seldom occurred. The importance of antibiotics and extracellular enzymes during hyphal interaction is discussed.

579 citations

01 Jan 1976
TL;DR: In this article, a filter paper assay method and unit value is described for the measurement of enzyme saccharification action, which is simple, reproducible, and quantitative and predicts enzyme action under practical conditions.
Abstract: A filter paper assay method and unit value is described for the measurement of enzyme saccharification action. The method is simple, reproducible, and quantitative and predicts enzyme action under practical saccharification conditions. (JSR)

505 citations

01 Jan 2011
TL;DR: Isolates of T. harzianum were found to differ in the levels of hydrolytic enzymes produced when mycelium of S. rolfsii, Rhizoctonia solani, and Pythium aphanidermatum in soil was attacked, correlated with the ability of each of the Trichoderma isolates to control the respective soilborne pathogens.

494 citations