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Journal ArticleDOI

Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of β-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum

01 Dec 1995-Enzyme and Microbial Technology (Elsevier)-Vol. 17, Iss: 12, pp 1043-1049
TL;DR: A temperature of 30°C and an initial pH of 4.7 were found to be optimal for β-1,3-glucanase production from T. harzianum in both surface culture and submerged culture processes.
About: This article is published in Enzyme and Microbial Technology.The article was published on 1995-12-01. It has received 38 citations till now. The article focuses on the topics: Trichoderma harzianum & Trichoderma reesei.
Citations
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Journal ArticleDOI
TL;DR: In this article, the effect of liquid:solid ratio, NaCl concentration and reaction time on the production of protein from germinant pumpkin seeds was investigated and the coefficient determination (R 2 ) was good for the second-order model.

227 citations

Journal ArticleDOI
TL;DR: This listing covers the period May 1, 1997 through to June 30, 1997, which roughly corresponds with the British Mycological Society's Special Interest Committees.

211 citations

Journal ArticleDOI
TL;DR: In this article, the effects of cultivation medium compositions including tapioca, fishmeal, CaCO3 and (NH4)2SO4 for the growth of Bacillus thuringiensis YMB 96-1988 were accessed by using response surface methodology (RSM).
Abstract: The effects of cultivation medium compositions including tapioca, fishmeal, CaCO3 and (NH4)2SO4 for the growth of Bacillus thuringiensis YMB 96-1988 were accessed by using response surface methodology (RSM). The two-level (24–1) fractional factorial designs (FFD) which involve two concentrations of each nutrient, and the paths of steepest ascent were effective in searching for the major factors of the bacteria growth. This allows the fitting of a first order linear model to the data. In this study, supplementary CaCO3 showed a negative effect on the spore production based on the first order regression coefficients derived from SAS programme. Subsequently, a 23 central composite design (CCD) was used for allocation of treatment combinations. Preliminary studies showed that tapioca and fishmeal is believed to be the major factors for the growth of B. thuringiensis. Estimated optimum compositions for the production of spores by B. thuringiensis are as follows: tapioca, 5.01%; fishmeal 5.86%; (NH4)2SO4 0.06% and resulted in a maximum spore count of 8.56 × 108/ml was obtained. This value is close to the 8.35 × 108/ml spore density as counted from actual experimental observations.

143 citations

Journal ArticleDOI
TL;DR: In this article, the effect of temperature, water to seed ratio and pH on the yield, apparent viscosity and emulsion stability index of wild sage seed hydrocolloid was investigated.
Abstract: The effect of temperature (25–80°C), water to seed ratio (25:1–85:1) and pH (3–9) on the yield, apparent viscosity and emulsion stability index of wild sage seed hydrocolloid was investigated. The generated quadratic model showed that the optimum conditions for maximizing the responses were when temperature was 25°C, water to seed ratio was 51:1 and pH was 5.5. All hydrocolloid solutions (1% w/v) showed shear thinning behavior in different extraction conditions, consistency coefficient and flow behavior index varied from 4.455 to 9.435 (Pa.sn), and 0.317 to 0.374, respectively. Besides, the chemical compositions of the seed and extracted gum were determined at optimal conditions.

74 citations


Cites methods from "Application of response surface met..."

  • ...[7] RSM is an effective statistical method that uses a minimum of resources and quantitative data from an appropriate experimental design to determine and simultaneously solve a multivariate equation.[13] Response surface experiments attempt to identify the response that can be thought of as a surface over the explanatory variables_ experimental space....

    [...]

Journal ArticleDOI
TL;DR: In this paper, reaction conditions were optimized for enzymatic hydrolysis of pectic substances using pectolytic enzymes produced by Aspergillus niger NCIM 548.

44 citations

References
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Journal ArticleDOI
TL;DR: Using Candida tenuis, a yeast isolated from the digestive tube of the larva of Phoracantha semipunctata, the bioconversion of citronellal to citroneLLol was demonstrated and the proposed second-order polynomial model was studied.
Abstract: Using Candida tenuis, a yeast isolated from the digestive tube of the larva of Phoracantha semipunctata (Cerambycidae, Coleoptera), we were able to demonstrate the bioconversion of citronellal to citronellol. Response surface methodology was used to achieve the optimization of the experimental conditions for that bioconversion process. To study the proposed second-order polynomial model, we used a central composite experimental design with multiple linear regression to estimate the model coefficients of the five selected factors believed to influence the bioconversion process. Only four were demonstrated to be predominant: the incubation pH, temperature, time, and the amount of substrate. The best reduction yields (close to 90%) were obtained with alkaline pH conditions (pH 7.5), a low temperature (25°C), a small amount of substrate (15 μl), and short incubation time (16 h). This methodology was very efficient: only 36 experiments were necessary to assess these conditions, and model adequacy was very satisfactory as the coefficient of determination was 0.9411.

65 citations

Journal ArticleDOI
TL;DR: A laminarinase has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate and was found to hydrolyze laminationarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosACcharides.
Abstract: A laminarinase [endo-(1,3)-β-d-glucanase] has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate. The enzyme was found to hydrolyze laminarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosaccharides. The enzyme was unable to cleave laminaribiose but hydrolyzed triose to laminaribiose and glucose. The enzyme cleaved laminaritetraose, yielding laminaritriose, laminaribiose, and glucose, and similarly cleaved laminaripentaose, yielding laminaritetraose, laminaritriose, laminaribiose, and glucose. The enzyme cleaved only glucans containing β-1,3 linkages. The pH and temperature optima were 4.8 and 55°C, respectively. Stability in the absence of a substrate was observed at temperatures up to 50°C and at pH values between 4.9 and 9.3. The molecular mass was determined to be 70 kilodaltons by sodium dodecyl sulfate-12.5% polyacrylamide gel electrophoresis, and the pI was 7.2. Enzyme activity was significantly inhibited in the presence of HgCl2, MnCl2, KMnO4, and N-bromosuccinimide. The Km of the enzyme on laminarin was 0.0016%, and the Vmax on laminarin was 3,170 μmol of glucose equivalents per mg of the pure enzyme per min.

55 citations

Journal ArticleDOI
TL;DR: The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 m

43 citations

Journal ArticleDOI
TL;DR: When compared with a range of colonists of straw and other potential antagonists of glasshouse and cereal pathogens, Trichoderma harzianum IMI 275950 exhibited the greatest biocontrol activity in vitro.
Abstract: When compared with a range of colonists of straw and other potential antagonists of glasshouse and cereal pathogens,Trichoderma harzianum IMI 275950 exhibited the greatest biocontrol activity in vitro. This activity was also observed in a gnotobiotic lettuce bioassay against the pathogenSclerotinia sclerotiorum. Activity was dependent on antagonist/pathogen inoculum ratio and temperature.

29 citations