Journal ArticleDOI
Applying CRISPR/Cas for genome engineering in plants: the best is yet to come
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TLDR
It will be desirable to apply the CRISPR/Cas nuclease system for the mutation of regulatory elements and for more complex genome rearrangements, like transcriptional regulators or DNA modifying enzymes, in the future.About:
This article is published in Current Opinion in Plant Biology.The article was published on 2017-04-01. It has received 234 citations till now. The article focuses on the topics: CRISPR & Genome engineering.read more
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Journal ArticleDOI
Shifting the limits in wheat research and breeding using a fully annotated reference genome
Rudi Appels,Rudi Appels,Kellye Eversole,Nils Stein,Nils Stein,Catherine Feuillet,Beat Keller,Jane Rogers,Curtis J. Pozniak,Frédéric Choulet,Assaf Distelfeld,Jesse Poland,Gil Ronen,Andrew G. Sharpe,Omer Barad,Kobi Baruch,Gabriel Keeble-Gagnère,Martin Mascher,Gil Ben-Zvi,Ambre-Aurore Josselin,Axel Himmelbach,François Balfourier,Juan J. Gutierrez-Gonzalez,Matthew J. Hayden,Chushin Koh,Gary J. Muehlbauer,Raj K. Pasam,Etienne Paux,Philippe Rigault,Josquin Tibbits,Vijay K. Tiwari,Manuel Spannagl,Daniel Lang,Heidrun Gundlach,Georg Haberer,Klaus F. X. Mayer,Danara Ormanbekova,Verena M. Prade,Hana Šimková,Thomas Wicker,David Swarbreck,Hélène Rimbert,Marius Felder,Nicolas Guilhot,Gemy Kaithakottil,Jens Keilwagen,Philippe Leroy,Thomas Lux,Sven Twardziok,Luca Venturini,Angéla Juhász,Michael Abrouk,Iris Fischer,Cristobal Uauy,Philippa Borrill,Ricardo H. Ramirez-Gonzalez,Dominique Arnaud,Smahane Chalabi,Boulos Chalhoub,Boulos Chalhoub,Aron T. Cory,Raju Datla,Mark W. Davey,John Jacobs,Stephen J. Robinson,Burkhard Steuernagel,Fred van Ex,Brande B. H. Wulff,Moussa Benhamed,Abdelhafid Bendahmane,Lorenzo Concia,David Latrasse,Jan Bartoš,Arnaud Bellec,Hélène Bergès,Jaroslav Doležel,Zeev Frenkel,Bikram S. Gill,Abraham B. Korol,Thomas Letellier,Odd-Arne Olsen,Kuldeep Singh,Miroslav Valárik,Edwin A. G. van der Vossen,Sonia Vautrin,Song Weining,Tzion Fahima,Vladimir Glikson,Dina Raats,Jarmila Číhalíková,Helena Toegelová,Jan Vrána,Pierre Sourdille,Benoit Darrier,D. Barabaschi,Luigi Cattivelli,Pilar Hernández,Sergio Gálvez,Hikmet Budak,Jonathan D. G. Jones,Kamil Witek,Guotai Yu,Ian Small,Joanna Melonek,Ruonan Zhou,Tatiana Belova,Kostya Kanyuka,Robert King,Kirby T. Nilsen,Sean Walkowiak,Richard D. Cuthbert,Ron Knox,Krysta Wiebe,Daoquan Xiang,Antje Rohde,Timothy Golds,Jana Čížková,Bala Ani Akpinar,Sezgi Biyiklioglu,Liangliang Gao,Amidou N’Daiye,Marie Kubaláková,Jan Šafář,Françoise Alfama,Anne-Françoise Adam-Blondon,Raphael Flores,Claire Guerche,Mikaël Loaec,Hadi Quesneville,Janet A. Condie,Jennifer Ens,Ron MacLachlan,Yifang Tan,Adriana Alberti,Jean-Marc Aury,Valérie Barbe,Arnaud Couloux,Corinne Cruaud,Karine Labadie,Sophie Mangenot,Patrick Wincker,Patrick Wincker,Gaganpreet Kaur,Ming-Cheng Luo,Sunish K. Sehgal,Parveen Chhuneja,O. P. Gupta,Suruchi Jindal,Parampreet Kaur,Palvi Malik,Priti Sharma,Bharat Yadav,Nagendra K. Singh,Jitendra P. Khurana,Chanderkant Chaudhary,Paramjit Khurana,Vinod Kumar,Ajay Kumar Mahato,Saloni Mathur,Amitha Mithra Sevanthi,Naveen Sharma,Ram Sewak Singh Tomar,Kateřina Holušová,Ondřej Plíhal,Matthew D. Clark,Matthew D. Clark,Darren Heavens,George Kettleborough,Jon Wright,Barbora Balcárková,Yuqin Hu,Elena A. Salina,Nikolai V. Ravin,Nikolai V. Ravin,Konstantin G. Skryabin,Konstantin G. Skryabin,Alexey V. Beletsky,Vitaly V. Kadnikov,Andrey V. Mardanov,Michail A. Nesterov,Andrey L. Rakitin,Ekaterina M. Sergeeva,Hirokazu Handa,Hiroyuki Kanamori,Satoshi Katagiri,Fuminori Kobayashi,Shuhei Nasuda,Tsuyoshi Tanaka,Jianzhong Wu,Federica Cattonaro,Min Jiumeng,Karl G. Kugler,Matthias Pfeifer,Simen Rød Sandve,Xu Xun,Bujie Zhan,Jacqueline Batley,Philipp E. Bayer,David Edwards,Satomi Hayashi,Zuzana Tulpová,Paul Visendi,Licao Cui,Xianghong Du,Kewei Feng,Xiaojun Nie,Wei Tong,Le Wang +207 more
TL;DR: This annotated reference sequence of wheat is a resource that can now drive disruptive innovation in wheat improvement, as this community resource establishes the foundation for accelerating wheat research and application through improved understanding of wheat biology and genomics-assisted breeding.
Journal ArticleDOI
Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield
Tang Li,Bigang Mao,Yaokui Li,Qiming Lv,Liping Zhang,Caiyan Chen,Hanjie He,Hanjie He,Weiping Wang,Xiongfeng Zeng,Shao Ye,Yinlin Pan,Hu Yuanyi,Yan Peng,Xiqin Fu,Hongqing Li,Shi-tou Xia,Bingran Zhao +17 more
TL;DR: This work supplies a practical approach to developing Cd pollution-safe indica rice cultivars that minimizes Cd contamination risk in grains by knocking out the metal transporter gene OsNramp5 using CRISPR/Cas9 system.
Journal ArticleDOI
Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus.
Anca Macovei,Neah R. Sevilla,Christian Cantos,Gilda B. Jonson,Inez H. Slamet-Loedin,Tomas Cermak,Daniel F. Voytas,Il-Ryong Choi,Prabhjit Chadha-Mohanty +8 more
TL;DR: The data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eif4G could not be maintained in homozygous state, and the final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence.
Journal ArticleDOI
CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing
TL;DR: Various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities are discussed.
Journal ArticleDOI
Gene Editing and Crop Improvement Using CRISPR-Cas9 System.
Leena Arora,Alka Narula +1 more
TL;DR: The review includes some information on traditional delivery methods of Cas9-gRNA complexes into plant cells and incorporates the advent ofCRISPR ribonucleoproteins (RNPs) that came up as a solution to various limitations that prevailed with plasmid-based CRISPR system.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TL;DR: In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.
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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor,Yongjoo Kim,Yongjoo Kim,Michael S. Packer,Michael S. Packer,John A. Zuris,John A. Zuris,David R. Liu,David R. Liu +8 more
TL;DR: E engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.
Journal ArticleDOI
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Luke A. Gilbert,Matthew H. Larson,Leonardo Morsut,Zairan Liu,Gloria A. Brar,Sandra Elizabeth Torres,Noam Stern-Ginossar,Onn Brandman,Evan H. Whitehead,Jennifer A. Doudna,Wendell A. Lim,Jonathan S. Weissman,Lei S. Qi +12 more
TL;DR: The results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISpri as a general tool for the precise regulation of gene expression in eukaryotic cells.
Journal ArticleDOI
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
F. Ann Ran,Patrick D. Hsu,Chie Yu Lin,Jonathan S. Gootenberg,Silvana Konermann,Alexandro E. Trevino,David A. Scott,Azusa Inoue,Shogo Matoba,Yi Zhang,Feng Zhang +10 more
TL;DR: In this paper, an approach that combines a Cas9 nickase mutant with paired guide RNAs to introduce targeted double-strand breaks is described. But the approach is limited to mouse zygotes.