Aspects of culture:Acanthamoeba astronyxis (Ray and Hayes 1954) from Bay of Bengal coasts, India
01 Jan 1987-Vol. 96, Iss: 1, pp 63-69
TL;DR: The overall growth showed little variation over ranges of media salinity (5–30‰), however, greater values were encountered in media of intermediate range, while mid-littoral and lowest low tide isolates preferred bay water agar and saline distilledWater agar media more.
Abstract: Acanthamoeba astronyxis strains, isolated from intertidal belts of Sagar Island (21°30′ to 21°50′ N and 88°04′ to 88°08′ E) facing Bay of Bengal, were cultured in saline distilled water agar medium supplemented withEscherichia coli as food bacterium. Population growth in bay water agar, cerophyl bay water agar and soil extract distilled water agar were compared with special reference to devised saline distilled water agar medium. The overall growth showed little variation over ranges of media salinity (5–30‰). However, greater values were encountered in media of intermediate range. Isolate from mangrove litter-soil of highest high tide belt (strains) grew more efficiently in cerophyl bay water agar and soil extract distilled water agar media while mid-littoral and lowest low tide isolates preferred bay water agar and saline distilled water agar media more.
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TL;DR: It has been found that the overall number of organisms per gram of soil attains peak value during the monsoon period, and this value comes down in post-monsoon samples and is the least in pre- monsoon ones.
Abstract: Acanthamoeba rhysodes has been found to be a predominant intertidal benthic gymnamoeba in the mangrove ecosystem of Sundarbans of lower deltaic Bengal, facing the Bay. The sampling zones under study were the highest high tide regions, with characteristic mangrove litter-soil, inundated twice per month during the highest ebb of spring tide. Population abundance of this species, both in its trophic and cystic forms in the three distinct seasonal periods of pre-monsoon (March to June), monsoon (July to October), and post-monsoon (November to February) has been surveyed for over two years. These seasonal periods affect the physico-chemical parameters of the habitat substrata, including temperature, pH, and salinity. It has been found that the overall number of organisms per gram of soil attains peak value during the monsoon period. This value comes down in post-monsoon samples and is the least in pre-monsoon ones.
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TL;DR: The genus Hartmannella Alexeieff is re-defined to include those species which assume a simple, monopodial limax-like form during locomotion and have nuclear division similar to that of metazoan cells and to distinguish it from the genus Acanthamoeba Volkonsky, 1931.
Abstract: SYNOPSIS. Seven species of limax amoebae were isolated into clonal, monoxenic cultures with Aerobacter aerogenes from material collected from freshwater habitats. Studies were made of their trophic structure, nuclear division, cyst structure, some aspects of cytochemistry, and other characteristics. Six new species are described: Vahlkampfia inornata, V. avara, V. jugosa, Hartmannella limacoides, H. vermiformis, and H. exundans. The well-known species Naegleria gruberi (Schardinger, 1899) is re-described on the basis of 8 strains; its flagellated phase was found to be biflagellate, with rare exceptions.
A correlation exists between the manner of locomotion and the pattern of nuclear division in the limax amoebae in the family Vahlkampfiidae and those in the genus Hartmannella.
Trophic amoebae of all species had a PAS-positive surface layer, altho results with H. vermiformis and H. exundans were less definite than with other species. All species except H. limacoides formed cysts in culture. The cyst walls of all cyst-forming species were strongly PAS-positive, but results of the zinc chloroiodide test for cellulose were negative with the method used.
The genus Hartmannella Alexeieff, 1912, is re-defined to include those species which assume a simple, monopodial limax-like form during locomotion and have nuclear division similar to that of metazoan cells and to distinguish it from the genus Acanthamoeba Volkonsky, 1931.
288 citations
228 citations
TL;DR: The observations indicate that the surface microlayers are largely heterotrophic microcosms, which can be as rich as laboratory cultures, and that an appreciable part of the dissolved organic carbon is carbohydrate of phytoplankton origin, released and brought to the surface by migrating and excreting phagotrophic protists.
Abstract: Dissolved organic carbon, carbohydrates, and adenosine triphosphate in the size fractions 0.2 to 3 micrometers and 3 to 1000 micrometers are significantly enriched in the upper 150-micrometer surface layer compared to subsurface water, mean enrichment factors being 1.6, 2.0, 2.5, and 3.1, respectively. When calculated as a 0.1-micrometer microlayer of wet surfactants, the mean concentration of organic matter was 2.9 grams per liter, of which carbohydrates accounted for 28 percent. The data for plant pigments and particulate adenosine triphosphate indicated that bacterioneuston was enriched at seven of nine stations while phagotrophic protists were enriched at five stations. Instances of enrichment and inhibition were verified by cultural data for bacteria and amoebas. The observations indicate that the surface microlayers are largely heterotrophic microcosms, which can be as rich as laboratory cultures, and that an appreciable part of the dissolved organic carbon is carbohydrate of phytoplankton origin, released and brought to the surface by migrating and excreting phagotrophic protists.
167 citations
01 Jan 1979
TL;DR: This manual contain8 a key to 15 families of freshwater and marine amoebae, of which only one, the Echinamoebidac, docs not contain a known marine species.
141 citations
79 citations