Autolysin(s) of Bacillus subtilis as dechaining enzyme.
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Bacillus subtilis bacteria grow exponentially as long filaments in well-aerated cultures at 48 C and these filaments are chains of cells which can become unlinked when a B. subtILis autolysin or egg white lysozyme is added to the culture medium.Abstract:
Bacillus subtilis bacteria grow exponentially as long filaments in well-aerated cultures at 48 C. These filaments are chains of cells which can become unlinked when a B. subtilis autolysin or egg white lysozyme is added to the culture medium.read more
Citations
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Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli.
Christoph Heidrich,Markus F. Templin,Astrid Ursinus,Melisa Merdanovic,Jürgen Berger,Heinz Schwarz,Miguel A. de Pedro,Joachim-Volker Höltje +7 more
TL;DR: It is reported that three amidases, named AmiA, B and C, exist in E. coli and that they are involved in splitting of the murein septum during cell division, and that the amidases were shown to act as powerful autolytic enzymes in the presence of antibiotics.
Journal ArticleDOI
The biosynthesis and functionality of the cell-wall of lactic acid bacteria
TL;DR: Diverse physiological functions may be assigned to the cell wall, which contribute to the technological and health-related attributes of lactic acid bacteria, for instance, phage receptor activity relates to the presence of specific substituents on teichoic acids and polysaccharides.
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The iap gene of Listeria monocytogenes is essential for cell viability, and its gene product, p60, has bacteriolytic activity.
TL;DR: The iap gene could not be inactivated without a loss of cell viability, indicating that p60 is an essential housekeeping protein for L. monocytogenes and probably also for other Listeria species.
Journal ArticleDOI
Autolytic enzyme-deficient mutants of Bacillus subtilis 168.
J E Fein,H J Rogers +1 more
TL;DR: The frequencies of transformation, the isolation of revertants, and observations with a temperature-sensitive mutant all point to the likelihood that the pleiotropic, phenotypic properties of the strains are due to a single mutation.
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LytM-Domain Factors Are Required for Daughter Cell Separation and Rapid Ampicillin-Induced Lysis in Escherichia coli
TL;DR: E. coli thus appears to rely on two distinct sets of putative PG hydrolases to promote proper cell division, and the phenotypes of mutants lacking LytM-domain factors bear a striking resemblance to those of mutants defective for the N-acetylmuramyl-l-alanine amidases.
References
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Journal ArticleDOI
Biological consequences of the replacement of choline by ethanolamine in the cell wall of Pneumococcus: chanin formation, loss of transformability, and loss of autolysis.
Journal ArticleDOI
Autolytic enzyme associated with cell walls of Bacillus subtilis.
TL;DR: Observations show that the enzyme responsible for autolysis of cell walls of the highly transformable strain 168 of Bacillus subtilis is an N-acylmuramyl-L-alanine amidase, and it is suggested that its action coupled with a transpeptidase is involved in the expansion of the cell wall.
Journal ArticleDOI
Properties of a Novel Pleiotropic Bacteriophage-Resistant Mutant of Staphylococcus aureus H
TL;DR: A phage-resistant mutant of Staphylococcus aureus H (SmR) was previously shown to lack polymeric teichoic acid as mentioned in this paper, and the mutant cells appeared to be covalently linked and could be separated by mild sonic energy.
Journal Article
Biochemical aspects of competence in the bacillus subtilis transformation system. ii. autolytic enzyme activity of cell walls.
Frank E. Young,John Spizizen +1 more
TL;DR: There are quantitative chemical differences between cell walls obtained from poorly and highly transformable strains of B. subtilis and the role of the cell wall in competence is investigated.
Journal ArticleDOI
Chaining and unchaining Streptococcus faecalis; a hypothesis of the mechanism of bacterial cell separation.
TL;DR: It was observed that in young broth cultures, Streptococcus faecalis (enterococcus) grows in marked chains which later break up rather abruptly; the chains can be maintained by spinning young cultures and resuspending the cells in fresh medium.