Automated sperm concentration analysis with a new flow cytometry-based device, S-FCM.
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94 citations
Cites background or methods from "Automated sperm concentration analy..."
...The poor precision of the Makler chamber also appears to affect the accuracy and it’s use in a validation study could lead to an underestimate the accuracy of the new method [19]....
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...In recent years, different types of haemocytometers have been used for validation of new protocols for determination of sperm concentration by flow cytometry [15–22]....
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...Several authors [15–20] have recently validated various procedures for flow cytometric determination of sperm concentration....
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34 citations
Cites background or methods from "Automated sperm concentration analy..."
...[8,31,34,35,37]; FSC + other 3 stains [33]; FSC + SSC + positive DNA stain of permeabilized cells [39]; FSC + SSC + 4 viability with DNA marker + cytoplasma marker; FSC + SSC + viability with two DNA 5 markers....
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...9 x 10 (6)/mL, were analysed [33], it is not clear whether 20 this rate would be higher when animal samples with higher sperm concentrations are analysed....
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...Sample preparation: undiluted; diluted [32]; diluted and stained [8,31,33-38]; diluted, 20 permeabilized, and stained [30,39,40]....
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18 citations
Cites background or methods from "Automated sperm concentration analy..."
...Calculation of sperm concentration5(sperm count4bead count)6dilution factors [10]6fluorescent bead stock concentration (825610(3)/mL)....
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...Flow cytometry has been used to investigate characteristics of sperm such as concentration [7,10], cell viability [11–13], plasma membrane integrity [14–16], mitochondrial function [17], capacitation and acrosomal status [18,19], Ca in sperm [20], chromatin structure Figure 2....
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...Moreover, it is possible that the degree of overestimation of sperm concentration is related to particles and debris present in ejaculate [30], because some authors have reported that flow cytometry is suitable for quantitative measurement of lower sperm concentrations [10]....
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References
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