Journal ArticleDOI
Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation.
Tristan Colomb,Etienne Cuche,Florian Charrière,Jonas Kühn,Nicolas Aspert,Frédéric Montfort,Pierre Marquet,Christian Depeursinge +7 more
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TLDR
A procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram, which enables one to reconstruct correct and accurate phase distributions, even in the presence of strong and high-order aberration.Abstract:
We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such as wave vectors, focal lengths, or distances. This method enables one to reconstruct correct and accurate phase distributions, even in the presence of strong and high-order aberrations. Examples of applications are shown for microlens imaging and for compensating for the deformations associated with a tilted thick plate. Finally we show that this method allows compensation for the curvature of the specimen, revealing its surface defects and roughness. Examples of applications are shown for microlenses and metallic sphere imaging.read more
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Wide-field, high-resolution Fourier ptychographic microscopy
TL;DR: An imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image, which can also correct for aberrations and digitally extend a microscope's depth-of-focus beyond the physical limitations of its optics.
Journal ArticleDOI
Digital holographic microscopy for live cell applications and technical inspection
Björn Kemper,Gert von Bally +1 more
TL;DR: Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells.
Journal ArticleDOI
Cell refractive index tomography by digital holographic microscopy.
Florian Charrière,Anca Marian,Frédéric Montfort,Jonas Kuehn,Tristan Colomb,Etienne Cuche,Pierre Marquet,Christian Depeursinge +7 more
TL;DR: Digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain for the first time, with a precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range.
Journal ArticleDOI
Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition
Jonas Kühn,Tristan Colomb,Frédéric Montfort,Florian Charrière,Yves Emery,Etienne Cuche,Pierre Marquet,Christian Depeursinge +7 more
TL;DR: In this paper, a technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented, where the vertical measurement range without phase ambiguity is extended to the micron range, thanks to the resulting synthetic wavelength defined by the beating of two wavelengths with a separation of about 80nm.
Journal ArticleDOI
Numerical parametric lens for shifting, magnification, and complete aberration compensation in digital holographic microscopy
Tristan Colomb,Frédéric Montfort,Jonas Kühn,Nicolas Aspert,Etienne Cuche,Anca Marian,Florian Charrière,Sébastien Bourquin,Pierre Marquet,Christian Depeursinge +9 more
TL;DR: It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, magnification, and especially complete aberration compensation, can be mimicked by numerical computation of a NPL.
References
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Journal ArticleDOI
Digital holography for quantitative phase-contrast imaging.
TL;DR: A new application of digital holography for phase-contrast imaging and optical metrology and an application to surface profilometry shows excellent agreement with contact-stylus probe measurements.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
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Spatial filtering for zero-order and twin-image elimination in digital off-axis holography
TL;DR: Off-axis holograms recorded with a CCD camera are numerically reconstructed with a calculation of scalar diffraction in the Fresnel approximation and the zero order of diffraction and the twin image are digitally eliminated by means of filtering their associated spatial frequencies in the computed Fourier transform of the hologram.
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Direct phase determination in hologram interferometry with use of digitally recorded holograms
TL;DR: In this paper, the phase determination in hologram interferometry is performed using a CCD target and phase estimation from the digitally sampled holograms, which are then used to calculate the phase of the interference phase.
Journal ArticleDOI
Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging
Pietro Ferraro,Sergio De Nicola,Andrea Finizio,Giuseppe Coppola,Simonetta Grilli,Carlo Magro,Giovanni Pierattini +6 more
TL;DR: An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes and it is shown that a correction effect can be obtained at all reconstruction planes.