Bacterial associations reveal spatial population dynamics in Anopheles gambiae mosquitoes.
Moritz Buck,Louise K. J. Nilsson,Carl Brunius,Roch K. Dabiré,Richard J. Hopkins,Richard J. Hopkins,Olle Terenius +6 more
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Analysis of the whole-body bacterial flora of An.Abstract:
The intolerable burden of malaria has for too long plagued humanity and the prospect of eradicating malaria is an optimistic, but reachable, target in the 21st century. However, extensive knowledge is needed about the spatial structure of mosquito populations in order to develop effective interventions against malaria transmission. We hypothesized that the microbiota associated with a mosquito reflects acquisition of bacteria in different environments. By analyzing the whole-body bacterial flora of An. gambiae mosquitoes from Burkina Faso by 16 S amplicon sequencing, we found that the different environments gave each mosquito a specific bacterial profile. In addition, the bacterial profiles provided precise and predicting information on the spatial dynamics of the mosquito population as a whole and showed that the mosquitoes formed clear local populations within a meta-population network. We believe that using microbiotas as proxies for population structures will greatly aid improving the performance of vector interventions around the world.read more
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Mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.
TL;DR: The results indicate that several mosquito species require living bacteria for development, and it is hypothesized that these species do not rely on particular bacteria because larvae do not reliably encounter the same bacteria in the aquatic habitats they develop in.
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The mosquito holobiont: fresh insight into mosquito-microbiota interactions.
Morgane Guégan,Karima Zouache,Colin Démichel,Guillaume Minard,Van Tran Van,Patrick Potier,Patrick Mavingui,Claire Valiente Moro +7 more
TL;DR: It is considered that conferring the term holobiont to the mosquito and its microbiota is useful to get a comprehensive understanding of the vector pathosystem functioning so as to be able to develop innovative and efficient novel vector control strategies.
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Diet‐related gut bacterial dysbiosis correlates with impaired development, increased mortality and Nosema disease in the honeybee (Apis mellifera)
Patrick Maes,Pedro A. P. Rodrigues,Randy Oliver,Brendon M. Mott,Kirk E. Anderson,Kirk E. Anderson +5 more
TL;DR: It is demonstrated that typically occurring alterations in diet quality play a significant role in colony health and the establishment of a dysbiotic gut microbiome and a systemic host effect.
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Wolbachia infections in natural Anopheles populations affect egg laying and negatively correlate with Plasmodium development
W. Robert Shaw,Perrine Marcenac,Lauren M. Childs,Caroline O. Buckee,Francesco Baldini,Simon P. Sawadogo,Roch K. Dabiré,Abdoulaye Diabaté,Flaminia Catteruccia +8 more
TL;DR: It is determined that reproductive manipulations induced by a recently identified Wolbachia strain that stably infects natural mosquito populations of a major malaria vector, Anopheles coluzzii, in Burkina Faso significantly accelerate egg laying but do not induce cytoplasmic incompatibility or sex-ratio distortion, two parasitic reproductive phenotypes that facilitate the spread of other Wolbachian strains within insect hosts.
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Large-scale untargeted LC-MS metabolomics data correction using between-batch feature alignment and cluster-based within-batch signal intensity drift correction
TL;DR: These procedures to address and correct for within- and between-batch variability in processing multiple-batch untargeted LC-MS metabolomics data to increase their quality provide unbiased measures of improved data quality, with implications for improved data analysis.
References
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MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: An advanced version of the Molecular Evolutionary Genetics Analysis software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, is released, which enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny.
Brief Communication MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: The Molecular Evolutionary Genetics Analysis (MEGA) software as discussed by the authors provides facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, including the inference of timetrees.
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UPARSE: highly accurate OTU sequences from microbial amplicon reads
TL;DR: The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% correct bases commonly reported by other methods.
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Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.
Koichiro Tamura,Masatoshi Nei +1 more
TL;DR: In this paper, a new mathematical method for estimating the number of transitional and transversional substitutions per site, as well as the total number of nucleotide substitutions was proposed, taking into account excess transitions, unequal nucleotide frequencies, and variation of substitution rate among different sites.
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Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea
Daniel P. R. Herlemann,Matthias Labrenz,Klaus Jürgens,Stefan Bertilsson,Joanna J Waniek,Anders F. Andersson +5 more
TL;DR: This study reports a first detailed bacterial inventory from vertical profiles of 60 sampling stations distributed along the salinity gradient of the Baltic Sea, one of world's largest brackish water environments, generated using 454 pyrosequencing of partial (400 bp) 16S rRNA genes.