Bicelles: a model membrane system for all seasons?
Citations
1,270 citations
482 citations
418 citations
Cites background or methods from "Bicelles: a model membrane system f..."
...Some of the key limitations of the original bicelle media are lack of tolerance to a wide range of biologically relevant conditions, and a limited life span (Ottiger & Bax, 1998; Sanders & Prosser, 1998)....
[...]
...Ordered bicelle domains exist only over well-defined limits of composition, temperature, and presumably pressure (Sanders et al. 1994; Sanders & Prosser, 1998)....
[...]
...Bicelles have thus far been the most widely used aligning media in high resolution NMR applications (Sanders & Prosser, 1998)....
[...]
...Both the number of lipids in the bicelle and the cooperative alignment of bicelles in the liquid crystal domain amplify the magnitude of the anisotropy tensor and result in very high levels of order, even at moderate field strengths (Sanders & Prosser, 1998)....
[...]
410 citations
346 citations
References
1,634 citations
"Bicelles: a model membrane system f..." refers background in this paper
...Variations in the scaling of order for water-soluble biomolecules can be achieved simply by varying the percentage of water in a bicellar sample [6,7,26]....
[...]
...Tjandra and Bax have recently used bicelles [6,7] to achieve a desired degree of sample alignment of ubiquitin and other water-soluble biomolecules and complexes....
[...]
...There is now considerable NMR data [5–7] to suggest that CHAPSO–DMPC and DHPC–DMPC bicelles do indeed provide a generally inert environment for water soluble proteins....
[...]
...Indeed, Tjandra and Bax were able to observe a few long-range 1H–1H dipolar couplings in their original study [6,7], foretelling what promises to be a bounteous future....
[...]
...The advantage of bicelles to free molecule alignment of water soluble species is that the net degree of orientation achievable at reasonable field strength is much higher, making detection and measurement of dipolar couplings and CSA much easier [6,7]....
[...]
1,497 citations
1,102 citations
752 citations
"Bicelles: a model membrane system f..." refers background in this paper
...An attempt has been made to draw the bicelles approximately to scale on the basis of the known dimensions of α helices, liquid crystalline phosphatidylcholine molecules [55], the thickness of DMPC bilayers [55] and the probable disc diameter of bicelles [39,40]....
[...]
750 citations