Journal ArticleDOI
Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
Eva Engvall,Erkki Ruoslahti +1 more
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TLDR
The findings suggest the possibility that Malignantly transformed fibroblasts lack surface fibronectin, which results in a lack of anchorage to the surrounding intercellular matrix, which could contribute to the malignant growth behavior.Abstract:
Fibronectin, a plasma protein immunologically identical with a major surface protein of normal fibroblasts, was found to bind to collagen and gelatin. A solid phase enzyme immunoassay was used for the binding tests. Collagen, gelatin or various control proteins were adsorbed to a plastic surface. Binding of fibronectin was detected using purified fibronectin antibodies conjugated to alkaline phosphatase. Circulating fibronectin and fibronectin obtained from fibroblast cultures both showed specific binding to collagen and gelatin. Preparative affinity chromatography of plasma on gelatin coupled to Sepharose gave electrophoretically and immunologically pure fibronectin in high yields. Malignantly transformed fibroblasts lack surface fibronectin. Our findings suggest the possibility that this results in a lack of anchorage to the surrounding intercellular matrix, which could contribute to the malignant growth behavior.read more
Citations
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Journal ArticleDOI
Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
Ronald A. Ignotz,Joan Massagué +1 more
TL;DR: The results demonstrate a functional involvement of fibronectin in mediating cellular responses to TGFbeta, and suggest a model for TGF beta action based on the control of the extracellular matrix in target cells.
Journal ArticleDOI
A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium
TL;DR: It is suggested that fibrous astrocytes and oligodendrocyte develop from a common progenitor cell and provide a striking example of developmental plasticity and environmental influence in the differentiation of CNS glial cells.
Journal ArticleDOI
How does the extracellular matrix direct gene expression
TL;DR: A model that postulates a “dynamic reciprocity” between the extracellular matrix (ECM) on the one hand and the cytoskeleton and the nuclear matrix on the other hand to alter the pattern of gene expression is presented.
Journal ArticleDOI
Fibronectins—adhesive glycoproteins of cell surface and blood
Kenneth M. Yamada,Kenneth Olden +1 more
TL;DR: A recently characterised class of adhesive, high molecular weight glycoproteins is present on the surfaces of cells, in connective tissue matrices, and in extracellular fluids.
Journal ArticleDOI
Role of collagenous matrices in the adhesion and growth of cells.
TL;DR: Current knowledge of the types of collagen and their distribution and biosynthesis is outlined, including fibronectin, which not only functions as an attachment protein, but also may play an important role in repair reactions.
References
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Book ChapterDOI
Enzyme-linked immunosorbent assay, ELISA
TL;DR: In this paper, the specificity of the DNP system was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
Journal Article
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
Eva Engvall,Peter Perlmann +1 more
TL;DR: In the DNP system, the specificity of the reaction was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
Journal ArticleDOI
Chemical Coupling of Proteins to Agarose
TL;DR: This method of activation and coupling is gentle and therefore particularly useful for the production of immunosorbents and insoluble enzymes.
Journal ArticleDOI
Alteration of cell-surface proteins by viral transformation and by proteolysis.
TL;DR: Putative cell-surface proteins of tissue-culture cells were identified by lactoperoxidase-catalyzed iodination, a technique that attaches label only to proteins outside the cell membrane, demonstrating that these proteins are cell derived, not contaminating serum proteins.
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