Binding of zona binding inhibitory factor-1 (ZIF-1) from human follicular fluid on spermatozoa.
11 Apr 2003-Journal of Biological Chemistry (American Society for Biochemistry and Molecular Biology)-Vol. 278, Iss: 15, pp 13570-13577
TL;DR: The carbohydrate moieties derived from ZIF-1 reduced the binding of native Zif-1 on human spermatozoa as well as the zona binding inhibitory activity of the glycoprotein, although the intensity of the effects are lower when compared with the native protein.
About: This article is published in Journal of Biological Chemistry.The article was published on 2003-04-11 and is currently open access. It has received 46 citations till now. The article focuses on the topics: Zona pellucida & Acrosome reaction.
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TL;DR: Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa, indicating that the carbohydrate moiety of glycodelin -S is critical for the function of the molecule.
98 citations
TL;DR: This review updates the recent research and clinical associations of glycodelin and highlights the role of glycosylation, which may be involved in the protection of spermatozoa against maternal immune reactions, and glyCodelin also has apoptogenic activity.
Abstract: Glycodelin is an example of a glycoprotein whose complex-type glycans mediate biological actions in human reproduction and immune reactions. Being attached to an identical protein backbone, glycodelin oligosaccharides vary significantly from one reproductive tissue to another and have an effect on its own secretion and role in cell communication. For instance, uterine glycodelin-A inhibits sperm-oocyte interaction by binding on the sperm head. This is a glycosylation-dependent phenomenon, in which fucosyltransferase-5 plays a key role. Glycodelin-S from seminal plasma binds evenly around the sperm head and maintains an uncapacitated state in the spermatozoa, until the isoform is detached during sperm passage through the cervix. Glycodelin-F from follicular fluid and Fallopian tube binds to the acrosomal region of the sperm head, thereby inhibiting both the sperm-oocyte binding and premature progesterone-induced acrosome reaction. The cumulus cells surrounding the oocyte can capture glycodelin-A and -F from the surrounding environment and convert these isoforms to a cumulus cell isoform, glycodelin-C. It differs by glycosylation from the other isoforms, and it too attaches on the sperm head, with the highest density in the equatorial region. Glycodelin-C is capable of detaching the sperm-bound inhibitory isoforms so that the sperm-oocyte binding is enhanced. Glycodelin-A also has immunosuppressive actions directed to cellular, humoral and innate immunity. Although these actions depend mainly on the protein backbone, glycosylation also plays a part. Glycosylated glycodelin may be involved in the protection of spermatozoa against maternal immune reactions, and glycodelin also has apoptogenic activity. Some glycosylation patterns of glycodelin may mask its apoptogenic domain. This review updates the recent research and clinical associations of glycodelin, highlighting the role of glycosylation.
94 citations
TL;DR: Observations suggest that human sperm FUT5 is a receptor of glycodelin-A and zona pellucida proteins, and that glycodzin-A inhibits spermatozoa-zona binding by blocking the binding of sperm Fut5 to the zonapellucIDA.
Abstract: Fertilization depends on successful binding of the spermatozoa to the zona pellucida of the oocyte. Glycodelin-A inhibits spermatozoa-zona pellucida binding. Previous data showed that glycodelin-A receptor(s) and zona pellucida protein receptor(s) on human spermatozoa are closely related. Using a chemical cross-linking approach, the glycodelin-A-sperm receptor complex was isolated. The receptor was identified to be fucosyltransferase-5 (FUT5) by mass spectrometry and confirmed with the use of anti-FUT5 antibodies. Sperm FUT5 was an externally oriented integral membrane protein in the acrosomal region of human spermatozoa. Biologically active FUT5 was purified from spermatozoa. Co-immunoprecipitation confirmed the interaction between glycodelin-A and sperm FUT5. Solubilized zona pellucida reduced the binding of glycodelin-A to sperm FUT5. An anti-FUT5 antibody and FUT5 acceptor blocked the binding of glycodelin-A to spermatozoa and the zona binding inhibitory activity of glycodelin-A. Sperm FUT5 bound strongly to intact and solubilized human zona pellucida. The equilibrium dissociation constant of sperm FUT5 binding to solubilized zona pellucida was 42.82 pmol/ml. These observations suggest that human sperm FUT5 is a receptor of glycodelin-A and zona pellucida proteins, and that glycodelin-A inhibits spermatozoa-zona binding by blocking the binding of sperm FUT5 to the zona pellucida.
76 citations
Additional excerpts
...33Research Article...
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TL;DR: Different isoforms of glycodelin have different biological roles on sperm function, and they act in succession to contribute to the success of fertilization.
62 citations
TL;DR: Glycodelin-C stimulated spermatozoazona pellucida binding in a dose-dependent manner, and it effectively displaced sperm-bound gly codelin-A and -F and enhances the zona binding capacity of spermatozosa passing through the cumulus oophorus.
62 citations
References
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01 Jan 1980
TL;DR: This laboratory manual consists of 2 sections which describe methods of examination of human semen and semen-cervical mucus interaction in order to standardize procedures and facilitate evaluation and comparison of research reports.
Abstract: This laboratory manual consists of 2 sections which describe methods of examination of human semen and semen-cervical mucus interaction in order to standardize procedures and facilitate evaluation and comparison of research reports The section on semen collection and examination discusses and makes recommendations for sample collection and delivery; initial examination; motility assessment estimation of sperm density; examination of particulate debris; agglutination; sperm viability; counting the spermatozoa; and analysis of morphological characteristics of germinal cells including preparation of seminal fluid smears staining method and classification and quantification of germinal cells and leucocytes Photomicrographs are provided to demonstrate morphological characteristics of normal and abnormal mature sperm immature germinal cells and leucocytes and epithelial cells Appendices provide information on frequency of various sperm forms in a normal ejaculate Papanicolaou staining procedure for sperm and the Bryan/Leishman stain for seminal fluid morphology smears A sample record for sperm analysis is also included The section on sperm-cervical mucus interaction describes the composition and characteristics of the mucus the collection procedure storage and preservation and evaluation including pH Methods of evaluating sperm-cervical mucus interaction are then described The timing and techniques of the post-coital test vaginal pool sample exocervical and low cervical samples and endocervical samples and their interpretation are discussed Instructions are provided for in vitro studies including the capillary tube test and the slide technique
2,141 citations
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TL;DR: This review analyzes the matter and suggests the hypothesis that at least some of the specificity of the selectins may involve recognition of "clustered saccharide patches."
Abstract: The selectins initiate many critical interactions among blood cells. The volume of information and diversity of opinions on the nature of the biologically relevant ligands for selectins is remarkable. This review analyzes the matter and suggests the hypothesis that at least some of the specificity may involve recognition of "clustered saccharide patches."
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TL;DR: The lipocalins are part of a larger protein superfamily: the calycins, which includes the fatty acid binding proteins, avidins, a group of metalloproteinase inhibitors, and triabin, and are characterised by a similar structure and by the conservation of a remarkable structural signature.
832 citations
TL;DR: It is noted time later with fusion of egg and sperm plasma mem-that there is good in vitro evidence for human sperm branes to form a single " activated " cell, the zygote.
468 citations