Biochemical studies on ghalakinoside a possible antitumor agent from Pergularia tomentosa
TL;DR: The present article includes the results of preliminary studies on the acute biochemical effects of this glycoside on certain blood parameters, especially those related to cancer diseases.
About: This article is published in Journal of Ethnopharmacology.The article was published on 1989-11-01. It has received 6 citations till now.
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TL;DR: Treatment of newly emerged fourth and fifth instar nymphs resulted in significant mortality and significant repellent activity with an LC50 value of 0.18 and 0.38%, respectively, after seven days of treatment with the crude methanolic extract of Pergularia tomentosa.
8 citations
TL;DR: The water fraction of root hydroalcoholic extract (PtR2), showed strong antiangiogenic effect with minimal adverse viability impacts, and may hold a considerable promise for an antiANGiogenic impact by impairment of endothelial cell (EC) migration and viability.
Abstract: Angiogenesis related abnormalities underlie several life-threatening disorders. Despite approved therapies, scientists have yet to develop highly efficient, low cost approaches with minimal side effects. We evaluated the antiangiogenic activity of 50% hydroalcoholic extracts of Pergularia tomentosa L. root and aerial parts along with their EtOAc and water fractions, in vivo and in vitro. Transgenic zebrafish line Tg(fli1:EGFP) was used for in vivo assay and human umbilical vein endothelial cell (HUVEC) migration test along with possibility of tube formation were performed as in vitro tests. Furthermore, microvasculature in chicken chorioallantoic membrane (CAM) was assessed under P. tomentosa treatment. The fractionation of the 50% hydroalcoholic extracts was led to the identification of the best active fraction in this study. The metabolite profiling of the active fraction was also carried out using LC-HRESIMS analysis. Pergularia tomentosa markedly inhibited intersegmental vessel (ISV) formation at 48 h post-fertilization (hpf) embryos in zebrafish. The water fraction of root hydroalcoholic extract (PtR2), showed strong antiangiogenic effect with minimal adverse viability impacts. Over 80% of embryos showed more than 50% inhibition in their ISV development at 20 and 40 μg/mL. PtR2 at 20 μg/mL substantially reduced human umbilical vein endothelial cell (HUVEC) migration up to 40%, considerable destruction of the formed tubes in the tube formation and microvasculature in CAM assays. Immunocytochemistry showed a marked reduction in vascular endothelial cadherin (VE-cadherin) abundance at cell junctions concurrent with substantial reduction of phospho-Akt (p-Akt) and β-catenin protein expressions. Phytochemical profile of PtR2 showed a rich source of cardenolide structures, including ghalakinoside, calactin and calotropin derivatives. Thus, the P. tomentosa cardenolide-rich fraction (PtR2) may hold a considerable promise for an antiangiogenic impact by impairment of endothelial cell (EC) migration and viability.
7 citations
TL;DR: The poisonous plants that grow in Qassim Region, and affect human and animal health are defined through questionnaires conducted with herbalists and owners of Perfumery shops "Attarren" deployed in the region as well as citizens of expertise and experience.
Abstract: This study aims to define the poisonous plants that grow in Qassim Region, and affect human and animal health. A total number of (42) species belonging to (39) genera and (23) families were recorded. These plants were encountered through questionnaires conducted with herbalists and owners of Perfumery shops "Attarren" deployed in the region as well as shepherds, nomads and citizens of expertise and experience in the field of poisonous plants in the region. Members of the family Poaceae (5 species) were reported as the most dominant toxic flowering plant family, followed by members of the families Chenopodiaceae and Boraginaceae (4 species each). These poisonous plants accounts for about 10 % of the total flora of the Region. For each species cited, botanical and vernacular names, family, toxic part and the toxic chemical principles are documented
3 citations
01 Jan 2002
TL;DR: Asclepiadaceae, known locally as “Ghalaka” (Ibn El Bitar 1809), is a plant indigenous to Saudi Arabia, Egypt, Abyssinia, North Africa, Iran, Afghanistan, West Pakistan, northern and central Sudan.
Abstract: Pergularia tomentosa L. (Daemia cordata Forssk, R. Br. ex Schult) Asclepiadaceae, known locally as “Ghalaka” (Ibn El Bitar 1809; Migahid 1989), is a plant indigenous to Saudi Arabia, Egypt, Abyssinia, North Africa, Iran, Afghanistan, West Pakistan, northern and central Sudan (Engler and Prantl 1891; Maire 1933; Migahid 1989). The plant is one of the abundant plants in Saudi Arabia, where it is found on rocky parts of deserts in various regions.
2 citations
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