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Book ChapterDOI

Biomolecule Characterization Using Laser Induced Breakdown Spectroscopy

TL;DR: In this paper, the LIBS spectra of biomolecules have been analyzed and interference from substrate interference was observed in the L 2 -emission lines of the biomolecule samples.
Abstract: In the present study, LIBS spectra of biomolecules have been analyzed. The basic biomolecules considered for the analysis were Carbohydrates, Protein and Nucleic acids. It was possible to detect the characteristic emission lines of C, H, N, O, S etc. in the above biomolecules. The samples were prepared for laser ablation as biofilms on glass slides. Interference from substrate was observed in the LIBS spectra of samples. Raman spectra would also be acquired in future studies to obtain molecular information for the biomolecules.
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Journal ArticleDOI
TL;DR: In this article, a high-power CO2 pulsed laser was used for the analysis of DNA bases Guanine and Adenine using a high resolution spectrometer. But the authors focused their attention on the temporal evolution of different atomic/ionic and molecular species.
Abstract: Laser-Induced Breakdown Spectroscopy (LIBS) of DNA bases Guanine and Adenine was studied using a high-power CO2 pulsed laser (λ=10.591 μm, τ FWHM=64 ns and fluences ranging from 25 to 70 J/cm2). The strong emission of the adenine and guanine plasma, collected using a high-resolution spectrometer, at medium-vacuum conditions (4 Pa) and at 1 mm from the target, exhibits excited molecular bands of CN (B2 Σ +–X2 Σ +) and excited neutral H and ionized N+ and C+. The medium-weak emission is due to excited species C2+, C3+, N, O, O+, O2+ and molecular band systems of $\mathrm{C}_{2}(\mathrm{d}^{3}\varPi_{\mathrm{g}}\mbox{--}\mathrm{a}^{3}\varPi_{\mathrm{u}};\ \mathrm{D}^{1}\varSigma_{\mathrm{u}}^{+}\mbox{--}\mathrm{X}^{1}\varSigma_{\mathrm{g}}^{+})$ , OH(A2 Σ +–X2 Π), NH(A3 Π–X3 Σ −), CH(A2 Π–X2 Π), $\mathrm{N}_{2}^{+}(\mathrm{B}^{2}\varSigma_{\mathrm{u}}^{+}\mbox{--} \mathrm{X}^{2}\varSigma_{\mathrm{g}}^{+})$ and N2(C3 Π u–B3 Π g). We focus our attention on the temporal evolution of different atomic/ionic and molecular species. The velocity distributions for various (different) species were obtained from time-of-flight (TOF) measurements. Intensities of some lines from C+ were used for determining electron temperature and their Stark-broadened profiles were employed to estimate the temporal evolution of electron density.

4 citations

Proceedings ArticleDOI
TL;DR: In this paper, results from remote LIBS and Raman analysis of biological samples such as amino acids, small peptides, mono-and disaccharides, and nucleic acids acquired under terrestrial and Mars conditions are reported, giving rise to some interesting differences.
Abstract: Laser-Induced Breakdown Spectroscopy (LIBS) and Raman Spectroscopy have rich histories in the analysis of a wide variety of samples in both in situ and remote configurations. Our team is working on building a deployable, integrated Raman and LIBS spectrometer (RLS) for the parallel elucidation of elemental and molecular signatures under Earth and Martian surface conditions. Herein, results from remote LIBS and Raman analysis of biological samples such as amino acids, small peptides, mono- and disaccharides, and nucleic acids acquired under terrestrial and Mars conditions are reported, giving rise to some interesting differences. A library of spectra and peaks of interest were compiled, and will be used to inform the analysis of more complex systems, such as large peptides, dried bacterial spores, and biofilms. These results will be presented and future applications will be discussed, including the assembly of a combined RLS spectroscopic system and stand-off detection in a variety of environments.

3 citations