Bioremediation of crude oil by Bacillus licheniformis in the presence of different concentration nanoparticles and produced biosurfactant
20 Jun 2017-International Journal of Environmental Science and Technology (Springer Berlin Heidelberg)-Vol. 14, Iss: 8, pp 1603-1614
TL;DR: This bacterial strain Bacillus licheniformis was considered as a good candidate to be applied in the bioremediation process of petroleum-contaminated sites using biosurfactant and specific concentration of (Fe2O3 and Zn5OH8Cl2) nanoparticles.
Abstract: This research work focuses on testing the bacterial strain Bacillus licheniformis for the bioremediation capacity of the crude oil. A biosurfactant and two different nanoparticles with different concentrations (0.05, 0.1, 0.2 g/100 ml) were applied separately to enhance the biodegradation process. The optimum biodegradation of crude oil was demonstrated at 60% of microcosms containing biosurfactant and nanoparticles after 7 days. The bacterial strain is highly potential to consume the total paraffins (iso- and n-paraffins) in crude oil samples. Accordingly, the best biodegradation of total paraffins was observed in microcosms containing (0.2 g) of Fe2O3, Zn5(OH)8Cl2 (nps) and biosurfactant separately. Additionally, the consumption of specific member rings of polyaromatics depends on the type and the concentration of nanoparticles. Thus, this bacterial strain was considered as a good candidate to be applied in the bioremediation process of petroleum-contaminated sites using biosurfactant and specific concentration of (Fe2O3 and Zn5OH8Cl2) nanoparticles.
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15 Feb 2019
TL;DR: This is the first report on yeast-mediated nanobioremediation of InP and optimization of the whole process using RSM, which demonstrated that InP degradation fitted first-order kinetic model.
Abstract: Seven yeast isolates were screened for the remediation of indeno(1,2,3-cd)pyrene (InP) using biosynthesized iron nanoparticles and produced biosurfactant in growth medium. Four yeast isolates showed positive response to produce biosurfactant which was confirmed by drop collapse test, emulsification index, methylene blue agar plate method, oil displacement test and lipase activity. The yeast strain showing maximum potential for InP degradation and biosurfactant production was identified as Candida tropicalis NN4. The produced biosurfactant was characterized as sophorolipid type through TLC and FTIR analysis. Iron nanoparticles were biosynthesized using mint leaf extract and characterized by various instrumental analysis. Response surface methodology (RSM), three-level five-variable Box–Behnken design (BBD) was employed to optimize the factors, viz., pH (7), temperature (30 °C), salt concentration (1.5 g L−1), incubation time (15 days) and iron nanoparticles concentration (0.02 g L−1) for maximum InP degradation (90.68 ± 0.7%) using C. tropicalis NN4. It was well in close agreement with the predicted value which was obtained by RSM model (90.68 ± 0.4%) indicating the validity of the model. InP degradation was confirmed through FTIR and GC–MS analysis. A kinetic study demonstrated that InP degradation fitted first-order kinetic model. This is the first report on yeast-mediated nanobioremediation of InP and optimization of the whole process using RSM.
28 citations
Cites background or methods from "Bioremediation of crude oil by Baci..."
...The fine powdered form of Fe nanoparticles was dispersed in ethanol by ultra-sonication and the sample was mounted on a micro-grid carbon film supported on a copper grid by placing a few droplets of a suspension followed by drying at 25 °C (El-Sheshtawy and Ahmed 2017)....
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...The effect of produced biosurfactant and specific concentration of (Fe2O3 and Zn5OH8Cl2) nanoparticles for remediation of crude oil has recently been reported by El-Sheshtawy and Ahmed (2017)....
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...The fine powdered form of Fe nanoparticles was dispersed in ethanol by ultra-sonication and the sample was mounted on a micro-grid carbon film supported on a copper grid by placing a few droplets of a suspension followed by drying at 25 °C (El-Sheshtawy and Ahmed 2017)....
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...El-Sheshtawy and Ahmed (2017) reported crude oil degradation using Bacillus licheniformis in presence of produced biosurfactant in the medium and specific concentration of (Fe2O3 and Zn5OH8Cl2) nanoparticles....
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TL;DR: Oil field adapted K. pneumoniae could efficiently degrade short-, medium- and long-chain alkanes in petroleum and thus is a potential source for advanced petroleum treatment.
Abstract: The removal of petroleum and petroleum-based products from the environment is of great importance. The objectives of this study were to investigate the most suitable physiological conditions and the effects of additional carbon, nitrogen and surfactant sources on petroleum biodegradation by Klebsiella pneumoniae ATCC13883 isolated from drilling fluid and to evaluate petroleum biodegradation with detailed hydrocarbon analysis by GC–MS. The results indicated that the highest biodegradation rate of 66.5% for K. pneumoniae was obtained under the conditions of pH 7, petroleum concentration 1% (v/v) and 7-day incubation at 150 rpm and 25 °C, proving to be the most effective physical conditions for petroleum biodegradation in this present study. Additional sources such as Triton X: 100, glucose and yeast extract significantly enhanced the petroleum biodegradation of K. pneumoniae to 68, 71 and 72.5%, respectively. In the last stage of this study, biodegradation rates were above 90% for hydrocarbons ranging from C10 and C20, above 70% for hydrocarbons ranging from C21 and C22 and above 40% for hydrocarbons ranging from C31 and C32. In conclusion, oil field adapted K. pneumoniae could efficiently degrade short-, medium- and long-chain alkanes in petroleum and thus is a potential source for advanced petroleum treatment.
18 citations
01 Jan 2019
TL;DR: In this article, magnetic nanomaterial-assisted purification of biosurfactants for oil recovery improvement from refinery sludge is also presented, where several types of bacteria and fungi produce bio-factants in response to growth in crude oil by using them as a carbon source.
Abstract: The petroleum industries have always faced major issues in the disposal of oil residues generated from storage, processing, and transportation facilities. Crude oil refining processes generate significant amounts of sludge, which is a complex mixture of alkanes, aromatic hydrocarbons, NSO (nitrogen-sulfur-oxygen containing compounds), and asphaltene fractions. Crude oil is usually stored in storage tanks before processing and due to structural complexity and low solubility, the impurities present in the crude oil are deposited at the bottom of the tank. The sludge is recovered from the tank during cleaning and disposed as waste. In India, oil refineries generate approximately 28,000 tons of oily sludge (a mixture of hazardous hydrocarbon waste) per annum. This waste residue is dumped into specially constructed sludge pits, consisting of a leachate collection system and a polymer lining system. This is to prevent the percolation of toxic hydrocarbon into the groundwater. However, these pits face drawbacks in that they are not only expensive, but are also difficult to construct and maintain, which requires more land. Current research shows that biosurfactant-enhanced recovery of oil from bottom tank sludge has been an ample scope for petroleum industries where two-step mechanisms were involved: mobilization and solubilization. Several types of bacteria and fungi produce biosurfactants in response to growth in crude oil by using them as a carbon source. These compounds offer greater potential in the recovery of oil from sludge, as they exhibit low-cost alternatives over the chemical surfactant. These biosurfactants are not only ecofriendly, but also help in the degradation of hydrocarbons. In this chapter, we address biosurfactant-producing bacteria and different types of biosurfactants. Furthermore, magnetic nanomaterial-assisted purification of biosurfactants for oil recovery improvement from refinery sludge is also presented.
13 citations
TL;DR: Two fungal isolates showed promising crude oil-degrading abilities with positive effect of low concentrations of AgNPs on biodegradation, proving RSM is an efficient mathematical method to optimize the microbial biodegrading of crude oil.
Abstract: Background: This research work focuses on the utilization of indigenous fungi for in situ bioremediation of crude oil in the presence of silver nanoparticles. Methods: Two fungi belonging to two different genera showed promising crude oil-degrading abilities. Fungal isolates were identified based on internal transcribed spacer rDNA sequence analysis. Gas chromatography-mass spectrometry analysis of the crude oil remaining in the culture medium after seven days was performed. The response surface method (RSM) designed by Box-Behnken was used to establish a mathematical model. Inter-simple sequence repeat (ISSR) primers were used to examine the genetic variation of fungal isolates. Results: Gas chromatography-mass spectrometry (GC-MS) analysis after seven days showed that the optimum biodegradation of crude oil was 57.8%. The crude oil degradation rate was significantly affected by a temperature of 30 °C, pH value of 7, crude oil concentration of 4 g/L, a 1:1 ratio between A. flavus AF15 and T. harzianum TH07, and an silver nanoparticle (AgNP) concentration of 0.05 g. Molecular characterization in fungal isolates is extremely valuable when using ISSR markers. Conclusions: Two fungal isolates showed promising crude oil-degrading abilities with positive effect of low concentrations of AgNPs on biodegradation. RSM is an efficient mathematical method to optimize the microbial biodegradation of crude oil.
12 citations
Cites background from "Bioremediation of crude oil by Baci..."
...It can be used to screen the degradation products of hydrocarbons, even C60 [43]....
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TL;DR: In this article, a 3-level Box-Behnken design was used to optimize the parameters for benzo[a]pyrene degradation using yeast consortium YC01 in presence of zinc oxide nanoparticles and produced biosurfactant in the growth medium.
11 citations
References
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TL;DR: Rates of biodegradation depend greatly on the composition, state, and concentration of the oil or hydrocarbons, with dispersion and emulsification enhancing rates in aquatic systems and absorption by soil particulates being the key feature of terrestrial ecosystems.
2,450 citations
TL;DR: Biosurfactants are more effective, selective, environmentally friendly, and stable than many synthetic surfactants, and the most promising applications are cleaning of oil-contaminated tankers, oil spill management, transportation of heavy crude oil, enhanced oil recovery, recovery of crude oil from sludge, and bioremediation of sites contaminated with hydrocarbons, heavy metals, and other pollutants.
Abstract: Many microorganisms, especially bacteria, produce biosurfactants when grown on water-immiscible substrates. Biosurfactants are more effective, selective, environmentally friendly, and stable than many synthetic surfactants. Most common biosurfactants are glycolipids in which carbohydrates are attached to a long-chain aliphatic acid, while others, like lipopeptides, lipoproteins, and heteropolysaccharides, are more complex. Rapid and reliable methods for screening and selection of biosurfactant-producing microorganisms and evaluation of their activity have been developed. Genes involved in rhamnolipid synthesis (rhlAB) and regulation (rhlI and rhlR) in Pseudomonas aeruginosa are characterized, and expression of rhlAB in heterologous hosts is discussed. Genes for surfactin production (sfp, srfA, and comA) in Bacillus spp. are also characterized. Fermentative production of biosurfactants depends primarily on the microbial strain, source of carbon and nitrogen, pH, temperature, and concentration of oxygen and metal ions. Addition of water-immiscible substrates to media and nitrogen and iron limitations in the media result in an overproduction of some biosurfactants. Other important advances are the use of water-soluble substrates and agroindustrial wastes for production, development of continuous recovery processes, and production through biotransformation. Commercialization of biosurfactants in the cosmetic, food, health care, pulp- and paper-processing, coal, ceramic, and metal industries has been proposed. However, the most promising applications are cleaning of oil-contaminated tankers, oil spill management, transportation of heavy crude oil, enhanced oil recovery, recovery of crude oil from sludge, and bioremediation of sites contaminated with hydrocarbons, heavy metals, and other pollutants. Perspectives for future research and applications are also discussed.
2,092 citations
TL;DR: Biosurfactants are amphiphilic compounds of microbial origin with considerable potential in commercial applications within various industries and have advantages over their chemical counterparts in biodegradability and effectiveness at extreme temperature or pH and in having lower toxicity.
Abstract: Surfactants are surface-active compounds capable of reducing surface and interfacial tension at the interfaces between liquids, solids and gases, thereby allowing them to mix or disperse readily as emulsions in water or other liquids. The enormous market demand for surfactants is currently met by numerous synthetic, mainly petroleum-based, chemical surfactants. These compounds are usually toxic to the environment and non-biodegradable. They may bio-accumulate and their production, processes and by-products can be environmentally hazardous. Tightening environmental regulations and increasing awareness for the need to protect the ecosystem have effectively resulted in an increasing interest in biosurfactants as possible alternatives to chemical surfactants. Biosurfactants are amphiphilic compounds of microbial origin with considerable potential in commercial applications within various industries. They have advantages over their chemical counterparts in biodegradability and effectiveness at extreme temperature or pH and in having lower toxicity. Biosurfactants are beginning to acquire a status as potential performance-effective molecules in various fields. At present biosurfactants are mainly used in studies on enhanced oil recovery and hydrocarbon bioremediation. The solubilization and emulsification of toxic chemicals by biosurfactants have also been reported. Biosurfactants also have potential applications in agriculture, cosmetics, pharmaceuticals, detergents, personal care products, food processing, textile manufacturing, laundry supplies, metal treatment and processing, pulp and paper processing and paint industries. Their uses and potential commercial applications in these fields are reviewed.
1,501 citations
TL;DR: It is suggested that earlier reports of biopolymers which both stabilized emulsions and lowered surface tension were actually similar aggregates of lipid and bioemulsifier.
Abstract: Two Bacillus species were studied which produced bioemulsifiers; however, they were distinctly different compounds. Bacillus sp. strain IAF 343 produced unusually high yields of extracellular biosurfactant when grown on a medium containing only water-soluble substrates. The yield of 1 g/liter was appreciably better than those of most of the biosurfactants reported previously. This neutral lipid product, unlike most lipid biosurfactants, had significant emulsifying properties. It did not appreciably lower the surface tension of water. On the same medium, Bacillus cereus IAF 346 produced a more conventional polysaccharide bioemulsifier, but it also produced a monoglyceride biosurfactant. The bioemulsifier contained substantial amounts of glucosamine and originated as part of the capsule layer. The monoglyceride lowered the surface tension of water to 28 mN/m. It formed a strong association with the polysaccharide, and it was necessary to use ultrafiltration to effect complete separation. The removal of the monoglyceride caused the polysaccharide to precipitate. It is suggested that earlier reports of biopolymers which both stabilized emulsions and lowered surface tension were actually similar aggregates of lipid and bioemulsifier.
1,136 citations
TL;DR: Cassava wastewater proved to be a suitable substrate for biosurfactant biosynthesis, providing not only bacterial growth and product accumulation but also a surfactant that has interesting and useful properties with potential for many industrial applications.
448 citations