BioTechniques 30th Anniversary GEM Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
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Cites background from "BioTechniques 30th Anniversary GEM ..."
...It is well known, that normalization by an internal reference gene reduce or even diminish tissue derived effects on qRT-PCR (Wittwer et al., 1997)....
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Cites background or methods from "BioTechniques 30th Anniversary GEM ..."
...Because the emissions from fluorescent chemistries are temperature dependent, data is generally acquired only once per cycle at the same temperature in order to monitor amplicon yield (45)....
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...The use of a pair of adjacent, fluorogenic hybridisation oligoprobes was first described in the late 1980s (43,44) and, now known as ‘HybProbes’, they have become the method of choice for the LightCyclerTM (Roche Molecular Biochemicals, Germany), a capillary-based, microvolume fluorimeter and thermocycler with rapid temperature control (20,45)....
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References
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