Journal ArticleDOI
Biotechnological Approaches for Production of Anti-Cancerous Compounds Resveratrol, Podophyllotoxin and Zerumbone.
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TLDR
This review focuses on the recent developments in the field of secondary metabolites from numerous plant sources, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants.Abstract:
Secondary metabolites from numerous plant sources have been developed as anti- cancer reagents and compounds such as resveratrol, podophyllotoxin and zerumbone are of particular importance in this regard. Since their de novo chemical synthesis is both arduous and commercially expensive, there has been an impetus to develop viable, biotechnological methods of production. Accordingly, this review focuses on the recent developments in the field, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants. Optimization of media and culture conditions, precursor feeding, immobilization and the use of chemical or physical elicitation in various protocols has led to an increase in resveratrol and podophyllotoxin production. Heterologous gene transformation of higher plants with stilbene synthase derived from Arachis hypogaea or Vitis vinifera lead to resveratrol production with the concomitant increase in resistance to plant pathogens. Interestingly, genetic transformation of Podophyllum hexandrum and Linum flavum with Agrobacterium rhizogenes resulted in Ri-T-DNA gene(s)-mediated enhancement of podophyllotoxin production. Zerumbone yields from tissue cultured plantlets or from suspension cultures are generally low and these methods require further optimization. In microbes lacking the native resveratrol or zerumbone biosynthesis pathway, metabolic engineering required not only the introduction of several genes of the pathway, but also precursor feeding and optimization of gene expression to increase their production. Data pertaining to safety and toxicity testing are needed prior to use of these sources of anti-cancer compounds in therapy.read more
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Journal ArticleDOI
Healthy Zerumbone: From Natural Sources to Strategies to Improve Its Bioavailability and Oral Administration
TL;DR: Zerumbone is a multifunctional compound with antimicrobial, antitumor, hyperalgesic, antioxidant and anti-inflammatory applications, and constitutes a point molecule for the future synthesis of derivatives with improved efficiency as mentioned in this paper .
Book ChapterDOI
Plant Tissue Culture and DNA Delivery Methods
TL;DR: Transgenic plants are developed through gene delivery into single cell/tissue, regeneration into whole plants that are fertile and adapt to the normal plant propagation process and Gene transfer techniques can greatly reduce the time taken in crop improvement against conventional breeding procedures and enlarge the array for desired plant improvement.
Journal ArticleDOI
Developing an in vitro elicitation strategy for specialized secondary metabolites production in adventitious root cultures of Primula veris subsp. veris
Virginia Sarropoulou,Eirini Sarrou,Andrea Angeli,Stefan Martens,Eleni Maloupa,Katerina Grigoriadou +5 more
TL;DR: In this paper , the authors presented the importance of abiotic elicitation strategies, and applied the first time in vitro adventitious root production systems, which are applied in detail on plant phenolic compounds including precursors and putrescine.
Role of polyamines on in vitro regeneration and podophyllotoxin production in Podophyllum hexandrum Royle
TL;DR: In this paper, the authors reported an efficient mass propagation protocol and Podophyllotoxin (PTOX) production in in vitro-derived plants of P. hexandrum, cultured on MS medium containing various plant growth regulators and polyamines.
Book ChapterDOI
Propagation of Podophyllum hexandrum Royale to Enhance Production of Podophyllotoxin
Utkarsha Srivastava,Hemant Sood +1 more
TL;DR: This review focuses on providing solutions to the researchers to develop new techniques to grow the plant in vitro as well as ex situ and gives an insight on the various methods that have been proved fruitful for increasing the production of podophyllotoxin in P. hexandrum.
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