Journal ArticleDOI
Butanediol production by Aerobacter aerogenes NRRL B199: Effects of initial substrate concentration and aeration agitation
J. M. Sablayrolles,Gérard Goma +1 more
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Butanediol production by Aerobacter aerogenes NRRL B199 grown on glucose requires an optimal rate of aeration for the obtention of butanediol 2, 3 and 4 and it has been observed that KLa increases during the fermentation cycle.Abstract:
Butanediol production by Aerobacter aerogenes NRRL B199 grown on glucose requires an optimal rate of aeration for the obtention of butanediol 2, 3. In the absence of air, Aerobacter aerogenes NRRL B199 growth and production are weak. Agitation-aeration is necessary for producing the biomass, but an excess of oxygen proves to be toxic with regard to metabolite production. Oxygen is a limiting substrate with regard to growth and an inhibitor with regard to the specific metabolite productivity. This observation is discussed from a kinetic stand point and in relation to the search for the optimum oxygen transfer coefficient (KLa), which is found to be in the range of 50–100h−1. It has also been observed that KLa increases during the fermentation cycle. The initial substrate concentration effects the yield production of biomass and butanediol production. Low yields of butanediol are obtained at low initial sugar concentrations, but good yields of butanediol are obtained (0.45 g/g) at high concentrations of glucose (195 g/L). Carbon substrates and butanediol are inhibitors of cell growth while butanediol is not quite an inhibitor of the specific rate of butanediol production for the range of butanediol of 0–100 g/L.read more
Citations
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Journal ArticleDOI
Microbial 2,3-butanediol production: A state-of-the-art review
TL;DR: Various strategies for efficient and economical microbial 2,3-butanediol production, including strain improvement, substrate alternation, and process development, are reviewed and compared with regard to their pros and cons.
Journal ArticleDOI
Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates
TL;DR: In this paper, the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment is described, including ethanol, butanol, lactic acid, acetic acid/vinegar, succinic acid, and fumaric acid.
Journal ArticleDOI
Fermentative production of 2,3-butanediol: A review
S.K. Garg,A. Jain +1 more
TL;DR: The production of 2,3-butanediol by bacterial species continues to be of great interest because of its varied application as discussed by the authors, and two bacterial species, Bacillus polymyxa and Klebsiella pneumoniae have demonstrated potential for butanediol fermentation on a commercial scale.
Journal ArticleDOI
Mechanism of enhanced oxygen transfer in fermentation using emulsified oxygen-vectors.
TL;DR: It has been demonstrated that the use of n‐dodecane emulsion in a culture of Aerobacter aerogenes enabled a 3.
Book ChapterDOI
The Microbial Production of 2,3-Butanediol
R. J. Magee,N. Kosaric +1 more
TL;DR: This chapter provides a comprehensive survey of diol production, including biochemistry, microbiology, and process engineering, and Klebsiella pneumoniae, with broad substrate and environmental adaptability, is the most thoroughly investigated organism.
References
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Journal ArticleDOI
Exocellular product formation by microorganisms in continuous culture. i. production of 2:3‐butanediol by aerobacter aerogenes in a single stage process
S. J. Pirt,D. S. Callow +1 more
TL;DR: A quantitative study of the growth and carbohydrate metabolism of Aerobacter aerogenes in continuous culture with particular reference to 2:3-butanediol production is described, with no sign of loss of butanediol-producing ability by any of the cultures.
Journal ArticleDOI
Production of 2,3-Butylene Glycol by Fermentation
Journal ArticleDOI
The Production of 2,3-Butylene Glycol by Aerobacter aerogenes 199.
B. H. Olson,Marvin J. Johnson +1 more
TL;DR: A study of the 2,3butylene glycol fermentation was made during the war because the glycol could serve as a starting material for the production of butadiene, and found that higher concentrations of carbohydrate could be fermented with a decreased fermentation time if air was passed through the medium.
Journal ArticleDOI
Production and properties of 2,3-butanediol: i. fermentation of wheat mashes by aerobacillus polymyxa
TL;DR: Isolation of Aerobacillus strains with desirable fermentative characteristics was facilitated by pasteurization of the original inoculum, both cultural characteristics and fermentative capacity of the originally isolates were extremely variable.
Journal ArticleDOI
Production and properties of 2, 3-butanediol: iii. studies on the biochemistry of carbohydrate fermentation by aerobacillus polymyxa
G. A. Adams,R. Y. Stanier +1 more
TL;DR: Carbon balances have been obtained for the fermentation of glucose, xylose, pyruvic acid, and mannitol by Aerobacillus polymyxa as discussed by the authors, where the main products from glucose are 2,3-butanediol, ethanol, carbon dioxide, and hydrogen.
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