Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.
Masaki Kinoshita,Michael Barber,William Mansfield,Yingzhi Cui,Daniel Spindlow,Giuliano Giuseppe Stirparo,Giuliano Giuseppe Stirparo,Sabine Dietmann,Jennifer Nichols,Austin Smith,Austin Smith +10 more
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TLDR
Stem cells derived from formative mouse epiblast show distinct transcription factor dependencies, relying critically on Otx2, and cell culture conditions applied to human naive cells or embryos support expansion of similar stem cells, consistent with a conserved staging post on the trajectory of mammalian pluripotency.About:
This article is published in Cell Stem Cell.The article was published on 2021-03-04 and is currently open access. It has received 112 citations till now. The article focuses on the topics: Induced pluripotent stem cell & Stem cell.read more
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Journal Article
Klf4 reverts developmentally programmed restriction of ground state pluripotency
Ge Guo,Jian Yang,Jian Yang,Jennifer Nichols,Jennifer Nichols,John Simon Hall,John Simon Hall,Isobel Eyres,William Mansfield,Austin Smith,Austin Smith +10 more
TL;DR: Mouse embryonic stem cells derived from pluripotent early epiblast contribute functionally differentiated progeny to all foetal lineages of chimaeras and were maintained after Cre-mediated deletion of the Klf4 transgene, formally demonstrating complete and stable reprogramming of developmental phenotype.
Journal ArticleDOI
Mammalian in vitro gametogenesis.
TL;DR: Germ cells differentiate into sexually dimorphic gametes, oocytes, and spermatozoa, which unite to form new individuals as discussed by the authors, and therefore, germ cell development entails intricate regulations of genome f...
Journal ArticleDOI
Formative pluripotent stem cells show features of epiblast cells poised for gastrulation.
Xiaoxiao Wang,Yunlong Xiang,Yang Yu,Ran Wang,Yu Zhang,Qianhua Xu,Sun Hao,Zhen Ao Zhao,Xiangxiang Jiang,Xiaoqing Wang,Xukun Lu,Dandan Qin,Yujun Quan,Jiaqi Zhang,Ng Shyh-Chang,Hongmei Wang,Naihe Jing,Naihe Jing,Wei Xie,Lei Li +19 more
TL;DR: In this article, self-renewing formative pluripotent stem cells (fPSCs) were established from different mouse ESCs, pre-/early-gastrula epiblasts and induced PSCs.
Journal ArticleDOI
A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre.
Matteo A. Molè,Matteo A. Molè,Tim H. H. Coorens,Marta N. Shahbazi,Marta N. Shahbazi,Antonia Weberling,Bailey A. T. Weatherbee,Carlos W. Gantner,Carmen Sancho-Serra,Lucy Richardson,Abbie Drinkwater,Najma Syed,Stephanie Engley,Philip Snell,Leila Christie,Kay Elder,Alison Campbell,Simon Fishel,Simon Fishel,Sam Behjati,Sam Behjati,Roser Vento-Tormo,Magdalena Zernicka-Goetz,Magdalena Zernicka-Goetz +23 more
TL;DR: In this article, the authors identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways and suggest that these cells can act as the anterior singalling centre to pattern the epiblast.
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Generation and characterization of stable pig pregastrulation epiblast stem cell lines.
Minglei Zhi,Jinying Zhang,Qianzi Tang,Dawei Yu,Shuai Gao,Dengfeng Gao,Pengliang Liu,Jianxiong Guo,Tang Hai,Jie Gao,Suying Cao,Zimo Zhao,Chongyang Li,Xiaogang Weng,Mengnan He,Tianzhi Chen,Yingjie Wang,Keren Long,Deling Jiao,Guanglei Li,Jiaman Zhang,Yan Liu,Yu Lin,Daxin Pang,Qianqian Zhu,Naixin Chen,Jingjing Huang,Xinze Chen,Yixuan Yao,Jingcang Yang,Zicong Xie,Xianya Huang,Mengxin Liu,Ran Zhang,Qiuyan Li,Yiliang Miao,Jianhui Tian,Xingxu Huang,Hongsheng Ouyang,Bofeng Liu,Wei Xie,Qi Zhou,Hong-Jiang Wei,Zhonghua Liu,Caihong Zheng,Mingzhou Li,Jianyong Han +46 more
TL;DR: In this article, a large-scale single-cell transcriptome analysis of pig embryos from embryonic day (E) 0 to E14, specifically, the tracing of pluripotency changes during epiblast development, was performed.
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