Journal ArticleDOI
CD1d-lipid antigen recognition by the γδ TCR
Adam P Uldrich,Jérôme Le Nours,Jérôme Le Nours,Daniel G. Pellicci,Nicholas A Gherardin,Kristy G McPherson,R.T. Lim,Onisha Patel,Travis Clarke Beddoe,Stephanie Gras,Jamie Rossjohn,Jamie Rossjohn,Jamie Rossjohn,Dale I. Godfrey +13 more
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TLDR
A population of human Vδ1+ γδ T cells that exhibit autoreactivity to CD1d is described and the molecular basis for how a γ Δ TCR bindsCD1d–α-galactosylceramide (α-GalCer) is provided.Abstract:
The T cell repertoire comprises αβ and γδ T cell lineages. Although it is established how αβ T cell antigen receptors (TCRs) interact with antigen presented by antigen-presenting molecules, this is unknown for γδ TCRs. We describe a population of human V δ 1 + γδ T cells that exhibit autoreactivity to CD1d and provide a molecular basis for how a γδ TCR binds CD1d-α- galactosylceramide (α-GalCer). The γδ TCR docked orthogonally, over the A′ pocket of CD1d, in which the V δ 1-chain, and in particular the germ line-encoded CDR1δ loop, dominated interactions with CD1d. The TCR γ-chain sat peripherally to the interface, with the CDR3γ loop representing the principal determinant for α-GalCer specificity. Accordingly, we provide insight into how a γδ TCR binds specifically to a lipid-loaded antigen-presenting molecule.read more
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Journal ArticleDOI
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Andrea Cossarizza,Hyun-Dong Chang,Andreas Radbruch,Andreas Acs,Dieter Adam,Sabine Adam-Klages,William W. Agace,William W. Agace,Nima Aghaeepour,Mübeccel Akdis,Matthieu Allez,Larissa Nogueira Almeida,Giorgia Alvisi,Graham Anderson,Immanuel Andrä,Francesco Annunziato,Achille Anselmo,Petra Bacher,Cosima T. Baldari,Sudipto Bari,Vincenzo Barnaba,Vincenzo Barnaba,Joana Barros-Martins,Luca Battistini,Wolfgang Bauer,Sabine Baumgart,Nicole Baumgarth,Dirk Baumjohann,Bianka Baying,Mary Bebawy,Burkhard Becher,Wolfgang Beisker,Vladimir Benes,Rudi Beyaert,Alfonso Blanco,Dominic A. Boardman,Christian Bogdan,Jessica G. Borger,Giovanna Borsellino,Philip E. Boulais,Jolene Bradford,Dirk Brenner,Dirk Brenner,Ryan R. Brinkman,Anna E. S. Brooks,Dirk H. Busch,Martin Büscher,Timothy P. Bushnell,Federica Calzetti,Garth Cameron,Ilenia Cammarata,Xuetao Cao,Susanna Cardell,Stefano Casola,Marco A. Cassatella,Andrea Cavani,Antonio Celada,Lucienne Chatenoud,Pratip K. Chattopadhyay,Sue Chow,Eleni Christakou,Eleni Christakou,Luka Cicin-Sain,Mario Clerici,Federico Colombo,Laura Cook,Anne Cooke,Andrea M. Cooper,Alexandra J. Corbett,Antonio Cosma,Lorenzo Cosmi,Pierre Coulie,Ana Cumano,Ljiljana Cvetkovic,Van Duc Dang,Chantip Dang-Heine,Martin S. Davey,Derek Davies,Sara De Biasi,Genny Del Zotto,Gelo Victoriano Dela Cruz,Michael Delacher,Silvia Della Bella,Paolo Dellabona,Günnur Deniz,Mark C. Dessing,James P. Di Santo,Andreas Diefenbach,Francesco Dieli,Andreas Dolf,Thomas Dörner,Regine J. Dress,Diana Dudziak,Michael L. Dustin,Charles-Antoine Dutertre,Charles-Antoine Dutertre,Friederike Ebner,Sidonia B G Eckle,Matthias Edinger,Pascale Eede,Götz R. A. Ehrhardt,Marcus Eich,Pablo Engel,Britta Engelhardt,Anna Erdei,Charlotte Esser,Bart Everts,Maximilien Evrard,Christine S. Falk,Todd A. Fehniger,Mar Felipo-Benavent,Helen Ferry,Markus Feuerer,Andrew Filby,Kata Filkor,Simon Fillatreau,Marie Follo,Irmgard Förster,John Bellamy Foster,Gemma A. Foulds,Britta Frehse,Paul S. Frenette,Stefan Frischbutter,Wolfgang Fritzsche,David W. Galbraith,David W. Galbraith,Anastasia Gangaev,Natalio Garbi,Brice Gaudilliere,Ricardo T. Gazzinelli,Ricardo T. Gazzinelli,Jens Geginat,Wilhelm Gerner,Nicholas A Gherardin,Kamran Ghoreschi,Lara Gibellini,Florent Ginhoux,Florent Ginhoux,Florent Ginhoux,Keisuke Goda,Keisuke Goda,Keisuke Goda,Dale I. Godfrey,Christoph Goettlinger,José M. González-Navajas,Carl S. Goodyear,Andrea Gori,Jane L. Grogan,Daryl Grummitt,Andreas Grützkau,Claudia Haftmann,Jonas Hahn,Hamida Hammad,Günter J. Hämmerling,Leo Hansmann,Göran K. Hansson,Christopher M. Harpur,Susanne Hartmann,Andrea Hauser,Anja E. Hauser,David L. Haviland,David W. Hedley,Daniela C. Hernández,Guadalupe Herrera,Martin Herrmann,Christoph Hess,Christoph Hess,Thomas Höfer,Petra Hoffmann,Kristin A. Hogquist,Tristan Holland,Thomas Höllt,Thomas Höllt,Rikard Holmdahl,Pleun Hombrink,Jessica P. Houston,Bimba F. Hoyer,Bo Huang,Fang-Ping Huang,Johanna Huber,Jochen Huehn,Michael Hundemer,Christopher A. Hunter,William Hwang,William Hwang,Anna Iannone,Florian Ingelfinger,Sabine Ivison,Hans-Martin Jäck,Peter K. Jani,Beatriz Jávega,Stipan Jonjić,Toralf Kaiser,Tomas Kalina,Thomas Kamradt,Stefan H. E. Kaufmann,Baerbel Keller,Steven L. C. Ketelaars,Ahad Khalilnezhad,Ahad Khalilnezhad,Srijit Khan,Jan Kisielow,Paul Klenerman,Jasmin Knopf,Hui-Fern Koay,Katja Kobow,Jay K. Kolls,Wan Ting Kong,Manfred Kopf,Thomas Korn,Katharina Kriegsmann,Hendy Kristyanto,Thomas Kroneis,Andreas Krueger,J. Kühne,Christian Kukat,Désirée Kunkel,Heike Kunze-Schumacher,Tomohiro Kurosaki,Christian Kurts,Pia Kvistborg,Immanuel Kwok,Immanuel Kwok,Jonathan J M Landry,Olivier Lantz,Paola Lanuti,Francesca LaRosa,Agnès Lehuen,Salomé LeibundGut-Landmann,Michael D. Leipold,Leslie Y. T. Leung,Megan K. Levings,Andreia C. Lino,Francesco Liotta,Virginia Litwin,Yanling Liu,Hans-Gustaf Ljunggren,Michael Lohoff,Giovanna Lombardi,Lilly Lopez,Miguel López-Botet,Amy E. Lovett-Racke,Erik Lubberts,Hervé Luche,Burkhard Ludewig,Enrico Lugli,Sebastian Lunemann,Holden T. Maecker,Laura Maggi,Orla Maguire,Florian Mair,Kerstin H. Mair,Alberto Mantovani,Rudolf A. Manz,Aaron J. Marshall,Alicia Martínez-Romero,Gloria Martrus,Ivana Marventano,Wlodzimierz Maslinski,Giuseppe Matarese,Anna Vittoria Mattioli,Christian Maueröder,Christian Maueröder,Alessio Mazzoni,James McCluskey,Mairi McGrath,Helen M. McGuire,Iain B. McInnes,Henrik E. Mei,Fritz Melchers,Susanne Melzer,Dirk Mielenz,Stephen D. Miller,Kingston H. G. Mills,Hans Minderman,Jenny Mjösberg,Jenny Mjösberg,Jonni S. Moore,Barry Moran,Lorenzo Moretta,Tim R. Mosmann,Susann Müller,Gabriele Multhoff,Luis E. Muñoz,Christian Münz,Toshinori Nakayama,Milena Nasi,Katrin Neumann,Lai Guan Ng,Antonia Niedobitek,Sussan Nourshargh,Gabriel Núñez,José-Enrique O'Connor,A Ochel,Anna E. Oja,Diana Ordonez,Alberto Orfao,Eva Orlowski-Oliver,Wenjun Ouyang,Annette Oxenius,Raghavendra Palankar,Isabel Panse,Kovit Pattanapanyasat,Malte Paulsen,Dinko Pavlinic,Livius Penter,Pärt Peterson,Christian Peth,Jordi Petriz,Federica Piancone,Winfried F. Pickl,Silvia Piconese,Marcello Pinti,A. Graham Pockley,Malgorzata J. Podolska,Zhiyong Poon,Katharina Pracht,Immo Prinz,Carlo Pucillo,Sally A. Quataert,Linda Quatrini,Kylie M. Quinn,Kylie M. Quinn,Helena Radbruch,Tim R. Radstake,Susann Rahmig,Hans-Peter Rahn,Bartek Rajwa,Gevitha Ravichandran,Yotam Raz,Jonathan Rebhahn,Diether J. Recktenwald,Dorothea Reimer,Caetano Reis e Sousa,Ester B. M. Remmerswaal,Lisa Richter,Laura G. Rico,Andy Riddell,Aja M. Rieger,J. Paul Robinson,Chiara Romagnani,Anna Rubartelli,Jürgen Ruland,Armin Saalmüller,Yvan Saeys,Takashi Saito,Shimon Sakaguchi,Francisco Sala-de-Oyanguren,Yvonne Samstag,Sharon Sanderson,Inga Sandrock,Angela Santoni,Ramon Bellmas Sanz,Marina Saresella,Catherine Sautès-Fridman,Birgit Sawitzki,Linda Schadt,Alexander Scheffold,Hans Scherer,Matthias Schiemann,Frank A. Schildberg,Esther Schimisky,Andreas Schlitzer,Josephine Schlosser,S Schmid,Steffen Schmitt,Kilian Schober,Daniel Schraivogel,Wolfgang Schuh,Thomas Schüler,Reiner Schulte,Axel Schulz,Sebastian R. Schulz,Cristiano Scottà,Daniel Scott-Algara,David P. Sester,T. Vincent Shankey,Bruno Silva-Santos,Anna Katharina Simon,Katarzyna M. Sitnik,Silvano Sozzani,Daniel E. Speiser,Josef Spidlen,Anders Ståhlberg,Alan M. 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Wing,Rieke Winkelmann,Thomas Winkler,Oliver F. Wirz,Alicia Wong,Peter Wurst,Jennie H M Yang,Jennie H M Yang,Juhao Yang,Maria Yazdanbakhsh,Liping Yu,Alice Yue,Hanlin Zhang,Yi Zhao,Susanne Ziegler,Christina E. Zielinski,Jakob Zimmermann,Arturo Zychlinsky +462 more
TL;DR: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community providing the theory and key practical aspects offlow cytometry enabling immunologists to avoid the common errors that often undermine immunological data.
Journal ArticleDOI
The burgeoning family of unconventional T cells.
Dale I. Godfrey,Dale I. Godfrey,Adam P Uldrich,Adam P Uldrich,James McCluskey,Jamie Rossjohn,Jamie Rossjohn,Jamie Rossjohn,D. Branch Moody +8 more
TL;DR: Evidence showing that unconventional T cells are an abundant component of the human immune system is reviewed and the immunotherapeutic potential of these cells and their antigenic targets are discussed.
Journal ArticleDOI
T cell antigen receptor recognition of antigen-presenting molecules.
Jamie Rossjohn,Stephanie Gras,John J. Miles,Stephen J. Turner,Dale I. Godfrey,James McCluskey +5 more
TL;DR: TCRs show remarkable structural and biological versatility in engaging different classes of Ag that are presented by polymorphic and monomorphic Ag-presenting molecules of the immune system.
Journal ArticleDOI
γδ T cells: first line of defense and beyond.
TL;DR: Functional and molecular analyses indicate that in infections, γδ T cells respond earlier than αβ T cells do and that they emerge late after pathogen numbers start to decline, suggesting that these cells may be involved in both establishing and regulating the inflammatory response.
Journal ArticleDOI
γδ T cells in cancer.
TL;DR: The current knowledge from both human and mouse studies are integrated to highlight the potential of γδ T cell modulation to improve cancer immunotherapy.
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