Journal ArticleDOI
cda1+, encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe.
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TLDR
Data indicated that cda1 + is required for proper spore formation in S. pombe, and found that the Cda1 protein self‐associated when its binding was tested both by two‐hybrid system and immunoprecipitation.About:
This article is published in FEBS Letters.The article was published on 2005-05-09. It has received 44 citations till now. The article focuses on the topics: Chitin deacetylase & Chitin synthase.read more
Citations
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Journal ArticleDOI
Chitin deacetylases: properties and applications.
TL;DR: Considering that chitin deacetylases play very important roles in the biological attack and defense systems, they may find applications for the biological control of fungal plant pathogens or insect pests in agriculture and for the biocontrol of opportunistic fungal human pathogens.
Journal ArticleDOI
The yeast spore wall enables spores to survive passage through the digestive tract of Drosophila.
TL;DR: The results suggest that the primary function of the yeast ascospore is as a cell type specialized for dispersion by insect vectors.
Journal ArticleDOI
Analysis of the phylogenetic relationships and evolution of the cell walls from yeasts and fungi
TL;DR: A phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall structure suggested that the components that made up the archaic walls were structural ones, forming a primitive girdle that retained noncovalently bound proteins in the periplasm and allowed cell growth in hypotonic media.
Journal ArticleDOI
Logical Identification of an Allantoinase Analog (puuE) Recruited from Polysaccharide Deacetylases
Ileana Ramazzina,Laura Cendron,Claudia Folli,Rodolfo Berni,Daniela Monteverdi,Giuseppe Zanotti,Riccardo Percudani +6 more
TL;DR: It is shown that an alternative allantoinase gene (puuE) can be precisely identified by analyzing its logic relationship with three other genes of the pathway by providing a structural rationale for the shifting of substrate specificity that occurred during evolution.
Journal ArticleDOI
Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases.
TL;DR: The current knowledge on the structure, activity, and specificity of CE4 enzymes is reviewed, focusing on chitin deacetylases and related enzymes active on N-acetylglucosamine-containing oligo and polysaccharides.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Book ChapterDOI
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
TL;DR: This chapter describes techniques concerned with classical and molecular genetics, cell biology, and biochemistry that can be used with Schizosaccharomyces pombe.
Journal ArticleDOI
Thiamine-repressible expression vectors pREP and pRIP for fission yeast
TL;DR: The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmid and the pRIP integrative expression plasmids.
Journal ArticleDOI
Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system.
TL;DR: RNA in situ hybridization and reverse transcriptase-PCR demonstrate a stage- and tissue-specific distribution for the expression of Th1, a member of a new family of basic helix-loop-helix (bHLH) transcription factors with a reduced binding specificity.
Journal ArticleDOI
The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle.
TL;DR: Genetic evidence indicated that a mutant lacking all three chitin synthases was inviable; this was confirmed by constructing a triple mutant rescued by a plasmid carrying a CHS2 gene under control of a GAL1 promoter.