CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification
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...However, methods of preparing cells for profiling have been applicable in practice to just hundreds (Hashimshony et al., 2012; Picelli et al., 2013) or (with automation) a few thousand cells (Jaitin et al., 2014), typically after first separating the cells by flow sorting (Shalek et al., 2013) or…...
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Cites methods from "CEL-Seq: Single-Cell RNA-Seq by Mul..."
...The method showed an excellent linear readout of the ERCC spike-in input concentration (Figure 4D) down to concentrations of 0.5 molecules/droplet on average; below that limit, we tended to over-count transcripts, a bias seen previously (Grün et al., 2014; Hashimshony et al., 2012)....
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...(Grün et al., 2014; Hashimshony et al., 2012)....
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...After barcoding, material from all cells is combinedbybreaking thedroplets, and thecDNA library is sequenced using established methods (CEL-seq) (Hashimshony et al., 2012; Jaitin et al., 2014)....
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References
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"CEL-Seq: Single-Cell RNA-Seq by Mul..." refers methods in this paper
...666 Cell Reports 2, 666–673, September 27, 2012 ª2012 The Author With the dramatically decreasing costs of sequencing, RNASeq (Wang et al., 2009) has emerged as the preferred method for transcriptomic analyses, overtaking microarrays, providing an imperative for any transcriptomic method to be adapted for RNA-Seq....
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...666 Cell Reports 2, 666–673, September 27, 2012 ª2012 The Author With the dramatically decreasing costs of sequencing, RNASeq (Wang et al., 2009) has emerged as the preferred method for transcriptomic analyses, overtaking microarrays, providing an imperative for any transcriptomic method to be…...
[...]
...666 Cell Reports 2, 666–673, September 27, 2012 a2012 The Author With the dramatically decreasing costs of sequencing, RNASeq (Wang et al., 2009) has emerged as the preferred method for transcriptomic analyses, overtaking microarrays, providing an imperative for any transcriptomic method to be adapted for RNA-Seq....
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