Journal ArticleDOI
Cellular uptake of the tat protein from human immunodeficiency virus
Alan D. Frankel,Carl O. Pabo +1 more
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Experiments using radioactive protein show that tat becomes localized to the nucleus after uptake and suggest that chloroquine protects tat from proteolytic degradation, raising the possibility that, under some conditions, tat might act as a viral growth factor to stimulate viral replication in latently infected cells or alter expression of cellular genes.About:
This article is published in Cell.The article was published on 1988-12-23. It has received 2733 citations till now. The article focuses on the topics: Viral replication & Cell culture.read more
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Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological Applications
Sophie Laurent,Delphine Forge,Marc Port,Alain Roch,Caroline Robic,Luce Vander Elst,Robert N. Muller +6 more
TL;DR: Practical Interests of Magnetic NuclearRelaxation for the Characterization of Superparamagnetic Colloid, and Use of Nanoparticles as Contrast Agents forMRI20825.
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Recent advances with liposomes as pharmaceutical carriers.
TL;DR: For further successful development of this field, promising trends must be identified and exploited, albeit with a clear understanding of the limitations of these approaches.
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A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus
TL;DR: The main determinants required for Tat translocation within this sequence are delineated by synthesizing several peptides covering the Tat domain from residues 37 to 60 and the domain extending from amino acid 37 to 47, which corresponds to the α-helix structure, is not required for cellular uptake and for nuclear translocation.
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Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
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Journal ArticleDOI
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
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Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.