Challenges in Cardiac Tissue Engineering
Gordana Vunjak-Novakovic,Nina Tandon,Nina Tandon,Amandine F. G. Godier,Robert Maidhof,Anna Marsano,Timothy P. Martens,Milica Radisic +7 more
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TLDR
The potential and challenges of cardiac tissue engineering for developing therapies that could prevent or reverse heart failure are reviewed.Abstract:
Cardiac tissue engineering aims to create functional tissue constructs that can reestablish the structure and function of injured myocardium. Engineered constructs can also serve as high-fidelity models for studies of cardiac development and disease. In a general case, the biological potential of the cell—the actual “tissue engineer”—is mobilized by providing highly controllable three-dimensional environments that can mediate cell differentiation and functional assembly. For cardiac regeneration, some of the key requirements that need to be met are the selection of a human cell source, establishment of cardiac tissue matrix, electromechanical cell coupling, robust and stable contractile function, and functional vascularization. We review here the potential and challenges of cardiac tissue engineering for developing therapies that could prevent or reverse heart failure.read more
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Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds
TL;DR: In this paper, the fundamental concepts of whole-organ engineering, including characterization of the extracellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such an acellular, naturally occurring three-dimensional biologic scaffold material that can then be seeded with selected cell populations.
Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional
TL;DR: The fundamental concepts of whole-organ engineering, including characterization of the extracellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such a scaffolds, techniques for the recellularization process and important aspects regarding bioreactor design to support this approach are described.
Journal ArticleDOI
3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts
TL;DR: A simple approach to 3D‐print thick, vascularized, and perfusable cardiac patches that completely match the immunological, cellular, biochemical, and anatomical properties of the patient is reported and cellularized human hearts with a natural architecture are printed.
Journal ArticleDOI
Tissue engineering by self-assembly and bio-printing of living cells.
Karoly Jakab,Cyrille Norotte,Francoise Marga,Keith E. Murphy,Gordana Vunjak-Novakovic,Gabor Forgacs +5 more
TL;DR: In this article, a fully biological, scaffoldless, print-based engineering approach that uses self-assembling multicellular units as bio-ink particles and employs early developmental morphogenetic principles, such as cell sorting and tissue fusion is presented.
Journal ArticleDOI
Engineered Microenvironments for Controlled Stem Cell Differentiation
TL;DR: The premise is that to unlock the full potential of stem cells, at least some aspects of the dynamic three-dimensional environments that are associated with their renewal, differentiation, and assembly in native tissues need to be reconstructed.
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