Journal ArticleDOI
Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences.
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TLDR
PCR-based restriction fragment length polymorphism and single-strand conformation polymorphism methods were established for the unequivocal delineation of the three species and should provide valuable tools for studying the life-cycle, transmission pattern(s), population structure and diagnosis of anisakiasis in humans and animals.About:
This article is published in International Journal for Parasitology.The article was published on 1998-12-01. It has received 249 citations till now. The article focuses on the topics: Spacer DNA & Internal transcribed spacer.read more
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Book ChapterDOI
Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes.
TL;DR: Examples in which anisakid nematodes recognized genetically at the species level in definitive and intermediate/paratenic hosts from various geographical areas of the Boreal and Austral regions and their infection levels have been used as biological indicators of fish stocks and food-web integrity in areas at high versus low levels of habitat disturbance are presented.
Journal ArticleDOI
Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism.
Stefano D'Amelio,Kostas D. Mathiopoulos,Cláudia Portes Santos,O.N Pugachev,S.C. Webb,M Picanço,Lia Paggi +6 more
TL;DR: The polymorphisms obtained by restriction fragment length polymorphisms have provided a new set of genetic markers for the accurate identification of sibling species and morphospecies.
Journal ArticleDOI
Molecular tools--advances, opportunities and prospects.
TL;DR: In particular, polymerase chain reaction (PCR)-coupled approaches have found broad applicability because their sensitivity permits the enzymatic amplification of gene fragments from minute quantities of nucleic acids from tiny amounts of parasite material as discussed by the authors.
Journal ArticleDOI
Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals.
TL;DR: Phylogenetic analysis of LSU sequences revealed strong support for the monophyly of Anisakinae, Contracaecum plus Phocascaris, Pseudoterranova, and Anisakis, which is primarily consistent with previously published phenograms based on multilocus electrophoretic data.
Journal ArticleDOI
Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes.
TL;DR: It is suggested that anisakiasis in Japan is mainly caused by A. simplex sensu stricto because it penetrates the muscle of the fish at a higher rate than A. pegreffii.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms
TL;DR: The mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms was developed and SSCPs were found to be allelic variants of true Mendelian traits, and therefore they should be useful genetic markers.
Journal ArticleDOI
Concerted evolution of repetitive dna sequences in eukaryotes
John F. Elder,Bruce J. Turner +1 more
TL;DR: It is argued that any natural grouping that is characterized by reproductive isolation and limited gene flow is capable of exhibiting concerted evolution of repetitive DNA arrays, and thus has important implications for the differentiation and discrimination of natural populations.