Journal ArticleDOI
Characterization and Biological Activities of Ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus.
Mariela M. Marani,Flávio Santos Dourado,Patrick Veras Quelemes,Alyne Rodrigues de Araújo,Márcia Luana Gomes Perfeito,Eder Alves Barbosa,Leiz Maria Costa Véras,Andreia Coelho,Andreia Coelho,Etielle B. Andrade,Etielle B. Andrade,Peter Eaton,João Paulo Figueiró Longo,Ricardo Bentes Azevedo,Cristina Delerue-Matos,José Roberto S. A. Leite +15 more
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TLDR
Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning and demonstrate the secretion of mature peptides derived from a pre-pro-peptide structure.Abstract:
Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ocellatins-PT6 to -PT8 (32 amino acid residues) have free carboxylates. Antimicrobial activity, hemolytic tests, and cytotoxicity against a murine fibroblast cell line were investigated. All peptides, except for ocellatin-PT2, have antimicrobial activity against at least one Gram-negative strain. Ocellatin-PT8 inhibited the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella choleraesuis strains with MICs in the 60–240 μM range. No significant effect was observed in human erythrocytes and in a murine fibroblast cell line after exposure to the peptides at MICs. A comparison bet...read more
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Journal ArticleDOI
Maleic acid as a dicarboxylic acid hydrotrope for sustainable fractionation of wood at atmospheric pressure and ≤100 °C: mode and utility of lignin esterification
TL;DR: In this paper, maleic acid (MA) was used as a green hydrotrope for efficient wood fractionation at atmospheric pressure and ≤100 °C, which resulted in esterified lignin with a low degree of condensation and a very light color.
Journal ArticleDOI
Antimicrobial peptides (AMPs): The quintessential ‘offense and defense’ molecules are more than antimicrobials
Seema Patel,Nadeem Akhtar +1 more
TL;DR: This review discusses the sources of therapeutic AMPs; auto-immunity risks of endogenous AMPs, and their dermatological applications; normally overlooked risks of the peptides; and scopes ahead.
Journal ArticleDOI
Machine learning-guided discovery and design of non-hemolytic peptides.
TL;DR: This work developed machine learning models and outlier detection methods that ensure robust predictions for the discovery of AMPs and the design of novel peptides with reduced hemolytic activity, and led to the discovery and design of 34 high-confidence non-hemolytic natural AMPs.
Journal ArticleDOI
Structure and function of a novel antioxidant peptide from the skin of tropical frogs.
Eder Alves Barbosa,Ana Oliveira,Alexandra Plácido,Renato Socodato,Camila C. Portugal,Ana Carolina Mafud,Alicia S. Ombredane,Daniel C. Moreira,Nuno Vale,Lucinda J. Bessa,Graziella Anselmo Joanitti,Cláudia Alves,Paula Gomes,Cristina Delerue-Matos,Yvonne Primerano Mascarenhas,Mariela M. Marani,João B. Relvas,Manuela Pintado,José Roberto S. A. Leite,José Roberto S. A. Leite +19 more
TL;DR: Results support the application of the peptide for neuroprotection and present a low cytotoxicity and suppressed menadione‐induced redox imbalance when tested with fibroblast in culture.
Journal ArticleDOI
Cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects.
Maria dos Remédios Mendes de Brito,Walter José Peláez,Martín S. Faillace,Gardenia C.G. Militão,Jackson Roberto Guedes da Silva Almeida,Gustavo A. Argüello,Zsolt Szakonyi,Ferenc Fülöp,Maria Cecília Barbosa da Silveira Salvadori,Fernanda de Sá Teixeira,Rivelilson Mendes de Freitas,Pedro Luiz Silva Pinto,Ana C. Mengarda,Marcos P. Silva,Ademar A. da Silva Filho,Josué de Moraes +15 more
TL;DR: Since schistosomiasis control programs rely on one drug, praziquantel, oxazines may have the potential to become new anticonvulsant and antitumor agents.
References
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