Characterization of age-related changes in bovine CD8+ T-cells.
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86 citations
Cites methods from "Characterization of age-related cha..."
...In some cases, positively or negatively selected gd T cells were cultured in plates coated with 10 mg/ml anti-bovine CD3 (clone MM1A; VMRD) and in the presence of 5 mg/ml anti-bovine CD28 (clone F849CD10; The Pirbright Institute) (33), 5 ng/ml rTGF-b1, and 50 U/ml human rIL-2 (Roche) as described previously (34)....
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Cites background from "Characterization of age-related cha..."
...It has been shown that active CTL express both perforin and Fas-ligand [1,46,47]....
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...Staining for perforin was done using anti-human-perforin-FITC (BD Pharmingen, San diego, CA, USA, cat# 556577) and staining for Fas-ligand was done using primary rabbit anti-Fas ligand (Santa Cruz biotechnology, cat no....
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...Tp1-tetramer positive CTL are also positive for CD8, FasL, and perforin It has been shown that active CTL express both perforin and Fas-ligand [1,46,47]....
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32 citations
Cites background from "Characterization of age-related cha..."
...The main advantage of MLV is that they replicate in the host’s target cells, so Ag is presented on MHC I (eliciting CTLs), as well as on MHC II (eliciting humoral immunity) (van Drunen Littel- van den Hurk, 2007)....
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...Bovine CD8 T cells express perforin (increasing with age) (Hogg et al., 2011) and have demonstrated MHC I-restricted killing in vitro (Guzman et al....
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...CTLs are considered im- portant for virus clearing and recovery from an infection, and Abs in the prevention of BHV-1 (re-)infection (Babiuk et al., 1996)....
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...CTLs likely play a role in control of recrudescence from latency in aHVs (Jones and Chowdhury, 2007)....
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...CTLs also carry the membrane-bound effector molecule Fas ligand (CD178), which binds to Fas (CD95) on a target cell to activate apoptosis in the Fas-bearing cell....
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