Journal ArticleDOI
Characterization of carbapenem-resistant Gram-negative bacteria from Tamil Nadu
Ramesh Nachimuthu,Ramkumar Subramani,Suresh Maray,Kodiveri Muthukaliannan Gothandam,Karthikeyan Sivamangala,Prasanth Manohar,Bülent Bozdogan +6 more
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TLDR
The results confirm the dissemination of NDM and emergence of OXA-181 beta-lactamase among Gram-negative bacteria in South India and showed the emergence of N DM producer in clinical isolates of E. hormaechei and P. rettgeri in India.Abstract:
Carbapenem resistance is disseminating worldwide among Gram-negative bacteria The aim of this study was to identify carbapenem-resistance level and to determine the mechanism of carbapenem resistance among clinical isolates from two centres in Tamil Nadu In the present study, a total of 93 Gram-negative isolates, which is found to be resistant to carbapenem by disk diffusion test in two centres, were included All isolates are identified at species level by 16S rRNA sequencing Minimal inhibitory concentrations (MICs) of isolates for Meropenem were tested by agar dilution method Presence of blaOXA, blaNDM, blaVIM, blaIMP and blaKPC genes was tested by PCR in all isolates Amplicons were sequenced for confirmation of the genes Among 93 isolates, 48 (%52) were Escherichia coli, 10 (%11) Klebsiella pneumoniae, nine (%10) Pseudomonas aeruginosa Minimal inhibitory concentration results showed that of 93 suspected carbapenem-resistant isolates, 27 had meropenem MICs ≥ 2 μg/ml The MIC range, MIC50 and MIC9read more
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The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model
TL;DR: The data shows that although in vitro studies showed high infectivity of phages, for in vivo models multiple phage doses were required for effective treatment.
Journal ArticleDOI
The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India.
Prasanth Manohar,Thamaraiselvan Shanthini,Ramankannan Ayyanar,Bülent Bozdogan,Aruni Wilson,Ashok J. Tamhankar,Ashok J. Tamhankar,Ramesh Nachimuthu,Bruno S. Lopes +8 more
TL;DR: The results highlight the distribution of carbapenem‐ and colistin‐resistance in Gram‐negative bacteria isolated from the Tamil Nadu region in South India.
Journal ArticleDOI
Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance
Ricaurte Alejandro Marquez-Ortiz,Leanne Haggerty,Narda Olarte,Carolina Duarte,Ulises Garza-Ramos,Jesus Silva-Sanchez,Betsy Esperanza Castro,Eby M. Sim,Mauricio Beltrán,Maria Victoria Moncada,Alberto Valderrama,Jaime E. Castellanos,Ian G. Charles,Natasha Vanegas,Natasha Vanegas,Javier Escobar-Perez,Nicola K. Petty +16 more
TL;DR: The findings suggest a Russian doll model for the dissemination of blaNDM-1 in Latin America, with P. rettgeri playing a central role in this process, and reveal new insights into the evolution and dissemination of plasmids carrying such antibiotic resistance genes.
Journal ArticleDOI
First literature review of carbapenem-resistant Providencia.
M. Abdallah,A. Balshi +1 more
TL;DR: Patients' characteristics, resistance mechanisms, treatment and infection control measures of carbapenem-resistant Providencia species in the literature are described.
Journal ArticleDOI
Activity of imipenem/relebactam against Pseudomonas aeruginosa producing ESBLs and carbapenemases.
Shazad Mushtaq,Danièle Meunier,Anna Vickers,Neil Woodford,David M. Livermore,David M. Livermore +5 more
TL;DR: Relebactam did potentiate imipenem against ESBL-producing P. aeruginosa, but this potentiation was generally insufficient to reduce imipanem MICs to the clinical range, just as for Enterobacterales.
References
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Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard
Matthew A. Wikler,Clinical +1 more
Journal ArticleDOI
Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Karthikeyan Kumarasamy,Mark Toleman,Timothy R. Walsh,Jay Bagaria,Fafhana Butt,Ravikumar Balakrishnan,Uma Chaudhary,Michel Doumith,Christian G. Giske,Seema Irfan,Padma Krishnan,Anil Kumar,Sunil Maharjan,Shazad Mushtaq,Tabassum Noorie,David L. Paterson,A. Pearson,Claire Perry,Rachel Pike,Bhargavi Rao,Ujjwayini Ray,Jayanta B. Sarma,Madhu Sharma,Elizabeth Sheridan,M A Thirunarayan,Jane F. Turton,Supriya Upadhyay,Marina Warner,William Welfare,David M. Livermore,Neil Woodford +30 more
TL;DR: The prevalence of NDM-1, in multidrug-resistant Enterobacteriaceae in India, Pakistan, and the UK is investigated, and co-ordinated international surveillance is needed.
Journal ArticleDOI
Characterization of a New Metallo-β-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India
Dongeun Yong,Mark Toleman,Christian G. Giske,Hyun Sun Cho,Kristina Sundman,Kyungwon Lee,Timothy R. Walsh +6 more
TL;DR: A Swedish patient of Indian origin traveled to New Delhi, India, and acquired a urinary tract infection caused by a carbapenem-resistant Klebsiella pneumoniae strain that typed to the sequence type 14 complex, showing broad resistance carried on these plasmids.
Journal Article
Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction
Journal ArticleDOI
Targeting virulence: a new paradigm for antimicrobial therapy
TL;DR: It is proposed that in addition to targeting virulence, new antimicrobial development strategies should be expanded to include targeting bacterial gene functions that are essential for in vivo viability.