Characterization of Cell Membrane Permeability In Vitro Part I: Transport Behavior Induced by Single-Pulse Electric Fields*:
01 Jan 2018-Technology in Cancer Research & Treatment (SAGE Publications)-Vol. 17, pp 1533033818792491-1533033818792491
TL;DR: The results provide evidence that cells, especially those that uptake propidium more slowly, can achieve large permeabilities with a single electrical pulse that may be quantitatively measured using standard fluorescence microscopy equipment and techniques.
Abstract: Most experimental studies of electroporation focus on permeabilization of the outer cell membrane Some experiments address delivery of ions and molecules into cells that should survive; others foc
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TL;DR: It is shown that after electroporation using electric pulse parameters that induce LirEP, some cells can be rescued by supplementing medium with compounds obtained from irreversibly electroporated cells, and it is determined that the intracellular molecules that contribute to the increase of cell viability are larger than 30 kDa.
20 citations
TL;DR: A kinetic model for the perforation of microalgae cells of the species Chlorella vulgaris was developed and is the first contribution showing the applicability of kinetic modeling and numerical simulation for designing PEF processes for the purpose of biorefiningmicroalgae biomass.
Abstract: Pulsed Electric Fields (PEF) is a promising technology for the gentle and energy efficient disruption of microalgae cells such as Chlorella vulgaris. The technology is based on the exposure of cells to a high voltage electric field, which causes the permeabilization of the cell membrane. Due to the dependency of the effective treatment conditions on the specific design of the treatment chamber, it is difficult to compare data obtained in different chambers or at different scales, e.g., lab or pilot scale. This problem can be overcome by the help of numerical simulation since it enables the accessibility to the local treatment conditions (electric field strength, temperature, flow field) inside a treatment chamber. To date, no kinetic models for the cell membrane permeabilization of microalgae are available what makes it difficult to decide if and in what extent local treatment conditions have an impact on the permeabilization. Therefore, a kinetic model for the perforation of microalgae cells of the species Chlorella vulgaris was developed in the present work. The model describes the fraction of perforated cells as a function of the electric field strength, the temperature and the treatment time by using data which were obtained in a milliliter scale batchwise treatment chamber. Thereafter, the model was implemented in a CFD simulation of a pilot-scale continuous treatment chamber with colinear electrode arrangement. The numerical results were compared to experimental measurements of cell permeabilization in a similar continuous treatment chamber. The predicted values and the experimental data agree reasonably well what demonstrates the validity of the proposed model. Therefore, it can be applied to any possible treatment chamber geometry and can be used as a tool for scaling cell permeabilization of microalgae by means of PEF from lab to pilot scale. The present work provides the first contribution showing the applicability of kinetic modeling and numerical simulation for designing PEF processes for the purpose of biorefining microalgae biomass. This can help to develop new processes and to reduce the costs for the development of new treatment chamber designs.
9 citations
TL;DR: In this paper, a mathematical model of electroporation considering the effect of IRE is proposed for the first time, where a typical two-dimensional cell system is discretized into nodes using MATLAB, and a mesh transport network method (MTNM) model is established for simulation.
7 citations
TL;DR: This paper critically examines the existing mechanistic, single-cell models which allow spatially and temporally resolved numerical simulations of electroporation-induced transmembrane transport of small molecules and shows that none of the tested models can be universally applied to the full range of experimental measurements.
6 citations
TL;DR: In this article , the authors examine the existing mechanistic, single-cell models which allow spatially and temporally resolved numerical simulations of electroporation-induced transmembrane transport of small molecules by confronting them with different experimental measurements.
6 citations
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"Characterization of Cell Membrane P..." refers methods in this paper
...51d).(29) Batch image processing was performed on each stack of images at each time point and each position with the chamber using CellProfiler (v2....
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"Characterization of Cell Membrane P..." refers methods in this paper
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"Characterization of Cell Membrane P..." refers background in this paper
...0).(26) Dirichlet boundary conditions were prescribed for the cylindrical regions at either end of the chamber that represent the electrode surfaces inserted into the chamber and set to the steady state voltage obtained from the 10-, 100-, and 1000-microsecond pulses (Supplemental Figure 1)....
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