Characterization of crude oil degrading bacterial communities and their impact on biofilm formation.
TL;DR: The uncultured bacterial strains are found to be playing a key role in the pitting type of corrosion and they can utilize crude oil hydrocarbons, which make them succeeded in extreme oil reservoir environments.
About: This article is published in Environmental Pollution.The article was published on 2021-10-01. It has received 19 citations till now. The article focuses on the topics: Biofilm.
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01 Jan 2007
TL;DR: In this paper, the role of Bacillus cereus ACE4 on degradation of diesel and its influence on corrosion of API============5LX steel was investigated and a demonstrating bacterial strain ACE4 was isolated from corrosion products and 16S============RNA gene sequence analysis showed that it has more than 99% similarity with B. cereus.
Abstract: The degradation problem of petroleum products arises since hydrocarbon acts as an excellent
food source for a wide variety of microorganisms. Microbial activity leads to unacceptable level
of turbidity, corrosion of pipeline and souring of stored product. The present study emphasizes
the role of Bacillus cereus ACE4 on degradation of diesel and its influence on corrosion of API
5LX steel. A demonstrating bacterial strain ACE4 was isolated from corrosion products and 16S
rRNA gene sequence analysis showed that it has more than 99% similarity with B. cereus. The
biodegradation and corrosion studies revealed that B. cereus degraded the aliphatic protons and
aromatic protons in diesel and is capable of oxidizing ferrous/manganese into oxides. This is the first
report that discloses the involvement of manganese oxidizer B. cereus ACE4 on biodegradation of
diesel and its influence on corrosion in a tropical country pipeline.
75 citations
TL;DR: The surface-active molecules along with the enzymes played a crucial role in the biodegradation process and changes in the major functional groups (CH, C-O-C, CO, =CH2, CH2,CH3) were confirmed by FTIR analysis and intermediated metabolites were identified by GCMS analysis.
46 citations
TL;DR: In this paper , the authors assembled multilayer antibacterial hybrids onto NaOH etched basalt scales via mussel-inspired depositions of PDA and AgNPs followed by post-modification with 1-Dodecanethiol.
6 citations
TL;DR: In this paper , an organo-metallic compound of the zinc sorbate (ZS) was successfully synthesized by the simple co-precipitation method and its improved antibacterial activity against sulfate-reducing bacteria (SRB).
6 citations
TL;DR: Considering the stability and economy of immobilized enzymes, a co-modified biochar immobilized laccase product named Fe3O4@NaBC@GA@LC via orthogonal experimental design and explored its possibility of remediating polycyclic aromatic hydrocarbons (PAHs) contaminated soil in steel plants as discussed by the authors .
6 citations
References
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TL;DR: It is confirmed that vulnibactin is assembled of both 2, 3-DHBA and SA, and conversion of SA to 2, 4-dihydroxybenzoic acid in presence of hydrogen peroxide and growth profile of AMP ligase mutants suggest a plausible existence of yet unidentified alternative siderophore that may be composed solely of 2,3-DH BA.
Abstract: Vibrio vulnificus is a halophilic estuarine bacterium that causes fatal septicemia and necrotizing wound infections in humans. Virulent V. vulnificus isolates produce a catechol siderophore called vulnibactin, made up of one residue of 2, 3-dihydroxybenzoic acid (2, 3-DHBA) and two residues of salicylic acid (SA). Vulnibactin biosynthetic genes (VV2_0828 to VV2_0844) are clustered at one locus of chromosome 2, expression of which is significantly up-regulated in vivo. In the present study, we decipher the biosynthetic network of vulnibactin, focusing specifically on genes around SA and 2, 3-DHBA biosynthetic steps. Deletion mutant of isochorismate pyruvate lyase (VV2_0839) or 2,3-dihydroxybenzoate-2,3-dehydrogenase (VV2_0834) showed retarded growth under iron-limited conditions though the latter showed more significant growth defect than the former, suggesting a dominant role of 2, 3-DHBA in the vulnibactin biosynthesis. A double deletion mutant of VV2_0839 and VV2_0834 manifested additional growth defect under iron limitation. Though the growth defect of respective single deletion mutants could be restored by exogenous SA or 2, 3-DHBA, only 2, 3-DHBA could rescue the double mutant when supplied alone. However, double mutant could be rescued with SA only when hydrogen peroxide was supplied exogenously, suggesting a chemical conversion of SA to 2, 3-DHBA. Assembly of two SA and one 2, 3-DHBA into vulnibactin was mediated by two AMP ligase genes (VV2_0836 and VV2_0840). VV2_0836 deletion mutant showed more significant growth defect under iron limitation, suggesting its dominant function. In conclusion, using molecular genetic analytical tools, we confirm that vulnibactin is assembled of both 2, 3-DHBA and SA. However, conversion of SA to 2, 3-DHBA in presence of hydrogen peroxide and growth profile of AMP ligase mutants suggest a plausible existence of yet unidentified alternative siderophore that may be composed solely of 2, 3-DHBA.
644 citations
TL;DR: Insight is provided into the life strategies of plant-associated endophytes and soil isolates of B. mycoides through the alteration of expression of an overlapping set of genes, which had been reported to be involved in plant–microbe interactions.
Abstract: Plant root secreted compounds alter the gene expression of associated microorganisms by acting as signal molecules that either stimulate or repel the interaction with beneficial or harmful species, respectively. However, it is still unclear whether two distinct groups of beneficial bacteria, non-plant-associated (soil) strains and plant-associated (endophytic) strains, respond uniformly or variably to the exposure with root exudates. Therefore, Bacillus mycoides, a potential biocontrol agent and plant growth-promoting bacterium, was isolated from the endosphere of potatoes and from soil of the same geographical region. Confocal fluorescence microscopy of plants inoculated with GFP-tagged B. mycoides strains showed that the endosphere isolate EC18 had a stronger plant colonization ability and competed more successfully for the colonization sites than the soil isolate SB8. To dissect these phenotypic differences, the genomes of the two strains were sequenced and the transcriptome response to potato root exudates was compared. The global transcriptome profiles evidenced that the endophytic isolate responded more pronounced than the soil-derived isolate and a higher number of significant differentially expressed genes were detected. Both isolates responded with the alteration of expression of an overlapping set of genes, which had previously been reported to be involved in plant-microbe interactions; including organic substance metabolism, oxidative reduction, and transmembrane transport. Notably, several genes were specifically upregulated in the endosphere isolate EC18, while being oppositely downregulated in the soil isolate SB8. These genes mainly encoded membrane proteins, transcriptional regulators or were involved in amino acid metabolism and biosynthesis. By contrast, several genes upregulated in the soil isolate SB8 and downregulated in the endosphere isolate EC18 were related to sugar transport, which might coincide with the different nutrient availability in the two environments. Altogether, the presented transcriptome profiles provide highly improved insights into the life strategies of plant-associated endophytes and soil isolates of B. mycoides.
623 citations
TL;DR: The proposed Mechanisms for Anaerobic Corrosion and Complex Ecosystems are presented, which include Sulphate-Reducing Bacteria and Anaerilic Corrosions, as well as proposed alternatives to these mechanisms.
Abstract: INTRODUCTION 195 CORROSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196 Electrochemical Mechanism . . . . . . . . . ....... ...... . . . . . . . . . . . . . ....... ... 196 Microbial Corrosion . . . . . . . .. .... . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . 197 Sulphate-Reducing Bacteria and Anaerobic Corrosion. . . . . . . . . . 199 Proposed Mechanisms for Anaerobic Corrosion . .. . . . . . . . . .. . .. ..... . . . . . . . . . . . ...... . . . . . . 200 Anaerobic Corrosion and Complex Ecosystems .. .. ....... . . . . . . . . . . . . . ........ 205
612 citations
TL;DR: Traditional water treatment strategies however, have largely failed to address those factors that promote biofouling activities and lead to biocorrosion.
Abstract: Corrosion associated with microorganisms has been recognized for over 50 years and yet the study of microbiologically influenced corrosion (MIC) is relatively new. MIC can occur in diverse environments and is not limited to aqueous corrosion under submerged conditions, but also takes place in humid atmospheres.Biofouling of industrial water systems is the phenomenon whereby surfaces in contact with water are colonized by microorganisms, which are ubiquitous in our environment. However, the economic implications of biofouling in industrial water systems are much greater than many people realize. In a survey conducted by the National Association of Corrosion Engineers of the United States ten years ago, it was found that many corrosion engineers did not accept the role of bacteria in corrosion, and many of them that did, could not recognize and mitigate the problem.Biofouling can be described in terms of its effects on processes and products such as material degradation (bio-corossion), product contaminatio...
391 citations
TL;DR: This article focuses on the growing understanding of bacteria and archaea responsible for the degradation of hydrocarbons under aerobic conditions in moderate to high salinity conditions and reveals that degradation of oxygenated and non-oxygenated hydrocarbon degradation by halophilic and halotolerant microorganisms occur by pathways similar to those found in non-halophiles.
Abstract: Many hypersaline environments are often contaminated with petroleum compounds. Among these, oil and natural gas production sites all over the world and hundreds of kilometers of coastlines in the more arid regions of Gulf countries are of major concern due to the extent and magnitude of contamination. Because conventional microbiological processes do not function well at elevated salinities, bioremediation of hypersaline environments can only be accomplished using high salt-tolerant microorganisms capable of degrading petroleum compounds. In the last two decades, there have been many reports on the biodegradation of hydrocarbons in moderate to high salinity environments. Numerous microorganisms belonging to the domain Bacteria and Archaea have been isolated and their phylogeny and metabolic capacity to degrade a variety of aliphatic and aromatic hydrocarbons in varying salinities have been demonstrated. This article focuses on our growing understanding of bacteria and archaea responsible for the degradation of hydrocarbons under aerobic conditions in moderate to high salinity conditions. Even though organisms belonging to various genera have been shown to degrade hydrocarbons, members of the genera Halomonas Alcanivorax, Marinobacter, Haloferax, Haloarcula, and Halobacterium dominate the published literature. Despite rapid advances in understanding microbial taxa that degrade hydrocarbons under aerobic conditions, not much is known about organisms that carry out similar processes in anaerobic conditions. Also, information on molecular mechanisms and pathways of hydrocarbon degradation in high salinity is scarce and only recently there have been a few reports describing genes, enzymes and breakdown steps for some hydrocarbons. These limited studies have clearly revealed that degradation of oxygenated and non-oxygenated hydrocarbons by halophilic and halotolerant microorganisms occur by pathways similar to those found in non-halophiles.
261 citations