Book ChapterDOI
Characterization of sialic acids.
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This article is published in Methods in Enzymology.The article was published on 1978-01-01. It has received 230 citations till now.read more
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Book ChapterDOI
Chemistry, Metabolism, and Biological Functions of Sialic Acids
TL;DR: This chapter discusses the chemistry, metabolism, and biological functions of sialic acids and the biosynthesis of N-acetylneuraminic acid is briefly reported and more attention is given to the enzyme reactions modifying this compound.
Journal ArticleDOI
The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal/GalNAc sequence.
Naoto Shibuya,Irwin J. Goldstein,Willem F. Broekaert,Makuta Nsimba-Lubaki,Benjamin Peeters,Willy J. Peumans +5 more
TL;DR: Carbohydrate binding properties of a new plant lectin isolated from elderberry bark were studied using the techniques of quantitative precipitation, hapten inhibition, and equilibrium dialysis, suggesting the presence of a hydrophibic interacting region adjacent to the carbohydrate binding site.
Journal ArticleDOI
Human plasma platelet-activating factor acetylhydrolase. Purification and properties.
TL;DR: Platelet-activating factor has a marked preference for the sn-2 acetyl residue and therefore can be considered as a specific PAF-acetylhydrolase, and the enzyme has an apparent molecular weight of 43,000, does not require calcium, has preference for micellar versus monomeric substrate, and exhibits surface dilution kinetics.
Journal ArticleDOI
Structure, biosynthesis and functions of glycoprotein glycans
Eric G. Berger,Eckhart Buddecke,Johannis P. Kamerling,Akira Kobata,James C. Paulson,Johannes F.G. Vliegenthart +5 more
TL;DR: This review may familiarize ‘newcomers’ with the field of glycoconjugate research with special emphasis on glycoprotein glycans and current knowledge on functional roles of glycans is presented.
Journal ArticleDOI
Mucin degradation in the human colon: production of sialidase, sialate O-acetylesterase, N-acetylneuraminate lyase, arylesterase, and glycosulfatase activities by strains of fecal bacteria.
TL;DR: It is concluded that the presence of two or more O-acetyl groups on sialic acids inhibits enteric bacterial sialidases but that production of sialate O-acetylesterases by several populations of enteric bacteria lessens the likelihood that mucin oligosaccharide chains terminating in O- acetylated sIALic acids are protected from degradation.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
Journal ArticleDOI
A modified colorimetric method for the estimation of n-acetylamino sugars
Journal ArticleDOI
Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
Journal ArticleDOI
The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application.
TL;DR: The present method, which is designed for use with short chain 0-acyl derivatives, is based on the finding that hydroxylamine at an alkaline pH in water rapidly converts acetylcholine stoichiometrically to hydroxamic acid throughout a wide range of ester concentration.