Journal ArticleDOI
Chemically modified porous silica gel as a bioadsorbent and a biocatalyst.
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TLDR
Epoxy silica gel, prepared by reaction of porous silica Gel with γ-glycidoxypropyltriethoxysilane, was coupled with m -aminobenzamidine and the resulting matrix was used for trypsin purification.About:
This article is published in Analytical Biochemistry.The article was published on 1979-09-15. It has received 12 citations till now. The article focuses on the topics: Silica gel & Human serum albumin.read more
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Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity
TL;DR: It was found that treatment of gels with dithiothreitol prior to impregnation with silver nitrate results in more reproducible staining patterns that are also qualitatively similar to those obtained with Coomassie blue.
Journal ArticleDOI
Chemiluminescence detection of proteins from single cells.
TL;DR: The AMPPD technique was demonstrated by the detection of proteins from individual photoreceptor outer segments, including proteins constituting approximately 1% of the total, and proved extremely sensitive for immunoblotting.
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Anti-T2 Monoclonal Antibody Immobilization On Quartz Fibers: Stability and Recognition of T2 Mycotoxin
TL;DR: The first two non-silanizing coupling methods are simple, inexpensive and non-hazardous compared to the third, more complex method in which an initial Correspondance: to PVS.
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A combination of magnetic permeability detection with nanometer-scaled superparamagnetic tracer and its application for one-step detection of human urinary albumin in undiluted urine.
TL;DR: A rapid (6.5 min) and simple one-step magnetic immunoassay (MIA) has been developed for analysis of human urinary albumin in near patient settings and the obtained results showed good correlation with the hospital immunoturbidimetric reference method.
Journal ArticleDOI
Characteristics of the biologically active 35-kDa metalloprotease virulence factor from Listeria monocytogenes
TL;DR: The environmental parameters necessary for increased production of mature, 35‐kDa active protease in strains of L. monocytogenes are described and its detection using polyclonal antibodies raised against Bacillus subtilis neutral protease is described.
References
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Enzymatic resolution. An organic-biochemical laboratory experiment.
G. E. Clement,R. Potter +1 more
TL;DR: An organic-biochemical laboratory experiment where α-chymotrypsin is used to resolve D,L-phenylalanine.