Figure 4. HUVEC cells expressing cell surface E-selectin: a) a cell under transmitted light; b) cell stained with BODIPY-labeled sLex monomer 23 ; c) cell stained with CD62E mAb followed by TRITC-conjugated anti mouse IgG; d) another cell under transmitted light; e) cell stained with BODIPY-labeled sLex dimer 2 ; f) cell stained with CD62E mAb followed by TRITC-conjugated anti mouse IgG. HUVEC cells negative for E-selectin expression did not exhibit staining with 2, 23, or CD62E mAb (data not shown).
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