Chromatographic investigation of the component glycerides of kusum oil
About: This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 6 citations till now.
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TL;DR: In this article, the application of thin-layer adsorption chromatography to the fractionation of complex lipid mixtures into classes was discussed, and further work has been done on the use of thin layer chromatography for this purpose.
Abstract: Further work has been done on the application of thin-layer adsorption chromatography to the fractionation of complex lipid mixtures into classes.
367 citations
TL;DR: In this paper, a procedure involving thin layer chromatography on silica impregnated with silver nitrate is described, which makes possible the separation of synthetic and natural glyceride mixtures into classes according to their degree of unsaturation, and within those classes the resolution of certain isomeric unsaturated glycerides.
Abstract: The paper describes a procedure involving thin layer chromatography on silica impregnated with silver nitrate, which makes possible the separation of synthetic and natural glyceride mixtures into classes according to their degree of unsaturation, and within those classes the resolution of certain isomeric unsaturated glycerides. The separations depend upon the ability of compounds having an olefinic double bond to form co-ordination complexes with the silver ion.
175 citations
TL;DR: In this paper, it is shown that with the help of these processes, it is possible to separate the critical pairs of fatty acids and to identify the individual components of triglycerides.
Abstract: Es wird uber Hydrierungs- und Bromierungs-Reaktionen berichtet, die direkt auf den dunnschicht-chromatographischen Platten durchgefuhrt werden, indem man das Palladium als Katalysator auf der Platte erzeugt bzw. das Brom in dem Fliesmittel lost. Mit Hilfe dieser Reaktionen ist est moglich, kritische Paare von Fettsauren zu trennen und einzelnen Komponenten zu identifizieren. Die Trennung der Brom-Derivate anstelle der ursprunglich ungesattigten Verbindungen fuhrte auch bei der Analyse der Triglyceride zu weiteren Fortschritten.
Thin Layer Chromatography in the Field of Fats VI: Hydrogenation and Bromination on the Plate
Hydrogenation and bromination employing palladium as catalyst on the plate and bromine dissolved in the mobile phase are carried out directly on the thin layer chromatographic plates. With the help of these processes it is possible to separate the critical pairs of fatty acids and to identify the individual components. The separation of bromo-derivatives instead of the original unsaturated compounds leads also to further progress in the analysis of triglycerides.
58 citations
TL;DR: In this article, a new method for the colouring of thin layer chromatographic plates has been described, which makes it possible to immerse the plates in aqueous solutions and hence the specific colouring procedures of the PC-analysis, which consist of a thorough washing of the chromatograms, are made applicable to the thin-layer chromatography.
Abstract: Es wird uber eine neue Moglichkeit zur Anfarbung dunnschichtchromatographischer Platten berichtet. Die Fixierung der Schicht mit Dichlordimethylsilan ermoglicht das Eintauchen der Platten in wasrige Losungen und somit die Ubertragung spezifischer Anfarbungsverfahren der pc-Analyse, die mit einem Auswaschen der Chromatogramme verbunden sind, auf die Dunnschicht-Chromatographie. Diese Fixierung fuhrte auch zur Erkennung der Substanzen mit Hilfe sog. Transparenz-Erscheinungen, auf die hier am Beispiel der Trennung von Triglyceriden naher eingegangen wird.
Thin Layer Chromatography in the Field of Fats III: Visualization of the Analysing Substances on the Plate
A new method for the colouring of thin layer chromatographic plates has been described. The fixing of the layer with dichlordimethylsilane makes it possible to immerse the plates in aqueous solutions and hence the specific colouring procedures of the pc-analysis, which consist of a thorough washing of the chromatograms, are made applicable to the thin layer chromatography. This fixation leads also to the detection of the substances with the help of the so-called transparency phenomenon, which has been dealt with here, taking triglyceride separation as an example.
35 citations
TL;DR: In this article, the triglyceride structures of Olive Oil and ester oil were calculated on the basis of 1,3-2 random distribution from total fatty acids and 2-monoglyceride fatty acids.
Abstract: Die nach der „1,3–2 random distribution” aus den Gesamtfettsauren und den 2-Monoglycerid-Fettsauren berechneten Triglycerid-Strukturen des Olivenoles und des Esteroles werden mit den durch Kombination von Silbernitrat- und Umkehrphasen-Chromatographie experimentell ermittelten Strukturen verglichen. Die Ergebnisse zeigen eine gute Ubereinstimmung mit den theoretischen Werten. Die Triglycerid-Struktur des Esteroles ist der des Olivenoles sehr ahnlich.
The Triglyceride Structures of Olive Oil and of a Glyceride Mixture Synthesised by Esterification of the Olive Oil Fatty Acids
The triglyceride structures of olive oil and the ester oil as calculated on the basis of 1,3–2 random distribution from total fatty acids and 2-monoglyceride fatty acids are compared with those found experimentally by the combination of silver nitrate and reverse phase chromatography. The results show a good agreement with the theoretical values. The triglyceride structure of the ester oil is very similar to that of olive oil.
22 citations