CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
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...However, this does not exclude the possibility of novel off-target activity occurring due to human genetic variation (Scott and Zhang, 2017; Tsai et al., 2017; Yang et al., 2014) or important consequences arising from mutations in genomic sites hitherto classified as benign....
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...…Addgene Plasmid #51132 Software and Algorithms CCTop webtool Stemmer et al., 2015 https://crispr.cos.uni-heidelberg.de/ CIRCLE-seq analysis software Tsai et al., 2017 https://github.com/tsailabSJ/circleseq/ Crispr.mit.edu webtool Hsu et al., 2013 http://crispr.mit.edu/ CRISPResso Pinello et al.,…...
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...CIRCLE-seq was performed as previously described (Tsai et al., 2017)....
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...…mutation sites in a genome-wide unbiased manner, we performed circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) (Tsai et al., 2017) on genomic DNA from human primary cells and identified 95 sites cleaved by the Cas9/CD33-gRNA complex in vitro (Figures S3D and…...
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...As a more comprehensive screen for all potential off-target mutation sites in a genome-wide unbiased manner, we performed circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) (Tsai et al., 2017) on genomic DNA from human primary cells and identified 95 sites cleaved by the Cas9/CD33-gRNA complex in vitro (Figures S3D and S3E)....
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