Circular dichroism and conformational polymorphism of DNA
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TLDR
Here the authors review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy, which significantly participated in all basic conformational findings on DNA.Abstract:
Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.read more
Citations
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DNA secondary structures: stability and function of G-quadruplex structures
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Nako Nakatsuka,Kyung-Ae Yang,John M. Abendroth,Kevin M Cheung,Xiaobin Xu,Hongyan Yang,Chuanzhen Zhao,Bowen Zhu,You Seung Rim,Yang Yang,Paul S. Weiss,Milan N. Stojanovic,Anne M. Andrews,Anne M. Andrews +13 more
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Topological Characterization of Nucleic Acid G‐Quadruplexes by UV Absorption and Circular Dichroism
Andreas Ioannis Karsisiotis,Nason Maani Hessari,Ettore Novellino,Gian Piero Spada,Antonio Randazzo,Mateus Webba da Silva +5 more
TL;DR: This work demonstrates the use of circular dichroism and UV techniques for characterizing three different types of G-quadruplex topologies classified through the sequence of glycosidic bond angles adopted by guanosines of the G- quadruplex stem.
Journal ArticleDOI
Heterochromatin protein 1α interacts with parallel RNA and DNA G-quadruplexes.
Ruby J Roach,Miguel Garavís,Carlos González,Geoffrey B. Jameson,Vyacheslav V. Filichev,Tracy K. Hale +5 more
TL;DR: It is shown that the unstructured hinge domain, necessary for the targeting of HP1α to constitutive heterochromatin, recognizes parallel G-quadruplex (G4) assemblies formed by the TElomeric Repeat-containing RNA (TERRA) transcribed from the telomere.
Journal ArticleDOI
Circular dichroism and guanine quadruplexes
Michaela Vorlíčková,Iva Kejnovská,Iva Kejnovská,Janos Sagi,Daniel Renčiuk,Daniel Renčiuk,Klára Bednářová,Jitka Motlová,Jaroslav Kypr,Jaroslav Kypr +9 more
TL;DR: It is shown that CD Spectroscopy is an important complementary technique to NMR spectroscopy and X-ray diffraction in quadruplex studies.
References
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