Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types
Citations
5,922 citations
Cites background or methods from "Circular RNAs Are the Predominant T..."
...We investigated published RNA-seq data from human leukocytes [19], mouse head, brain, and differentiation induced stem cells [11, 25], as well as our own data for human HEK293 cells and the early stages of C....
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...Intrigued by the circularity of the naturally occurring CDR1as RNA, we asked how many other circular RNAs exist in animals, and what are the properties of circular RNAs (circRNAs) that distinguish them from linearRNA?Previouswork had already identified naturally occurring circRNAs in human leukocytes [19], archaea [5], and the Sry transcript expressed in mouse testis [3]....
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...The latter point stresses the advantage of our detection strategy, which in contrast to [19] does not rely on existing exon annotations....
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5,885 citations
3,310 citations
Cites background or result from "Circular RNAs Are the Predominant T..."
...These results are consistent with prior studies of other RNA circles (Nigro et al. 1991; Salzman et al. 2012) and suggest that ecircRNAs either undergo nuclear export or are released to the cytoplasm during mitosis, where they enjoy extraordinary stability, likely as a result of resistance to debranching enzymes and RNA exonucleases....
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...While a bioinformatic approach to RNA circle identification has recently been described (Salzman et al. 2012), we reasoned that a biochemical enrichment of nonlinear RNAs might allow for the detection of more rare and diverse circular RNA forms....
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...As was the case for human cells, a high number (646 of 1477) of circles found through a prior bioinformatic analysis (Salzman et al. 2012) of murine brain were identified by CircleSeq of murine testis....
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...Most recently, Brown and colleagues reported the identification of >1000 transcription products favoring circularization (Salzman et al. 2012)....
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...This method appears very sensitive; the 25,166 replicated backsplice events detected by CircleSeq included the large majority (1025 of 1319) of putative circles identified through a previously described bioinformatic approach (Salzman et al. 2012)....
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References
2,107 citations
"Circular RNAs Are the Predominant T..." refers background in this paper
.... A role in regulating the pool of RNA binding proteins or small RNAs capable of interacting with the conventional linear RNA counterpart is another possibility...
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949 citations
"Circular RNAs Are the Predominant T..." refers background in this paper
...In the adult testes, the RNA exists primarily as a circular product that is predominantly localized to the cytoplasm and is apparently not translated [5,6]....
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Additional excerpts
...with risk for developing atherosclerosis [16]....
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