Journal ArticleDOI
CLARITY for mapping the nervous system
Kwanghun Chung,Karl Deisseroth +1 more
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TLDR
Hydrogel-based structures can be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue, creating a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules.Abstract:
With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies.read more
Citations
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Journal ArticleDOI
Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing
Bin Yang,Jennifer B. Treweek,Rajan P. Kulkarni,Rajan P. Kulkarni,Benjamin E. Deverman,Chun-Kan Chen,Eric Lubeck,Sheel Shah,Long Cai,Viviana Gradinaru +9 more
TL;DR: Techniques for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically transparent, thereby exposing their cellular structure with intact connectivity are presented.
Journal ArticleDOI
Intact-Brain Analyses Reveal Distinct Information Carried by SNc Dopamine Subcircuits.
Talia N. Lerner,Carrie Shilyansky,Thomas J. Davidson,Kathryn E. Evans,Kevin T. Beier,Kelly A. Zalocusky,Ailey K. Crow,Robert C. Malenka,Liqun Luo,Raju Tomer,Karl Deisseroth +10 more
TL;DR: Two parallel nigrostriatal dopamine neuron subpopulations differing in biophysical properties, input wiring, output wiring to dorsomedial striatum versus dorsolateral striatum (DLS), and natural activity patterns during free behavior are identified.
Journal ArticleDOI
Zebrafish models for translational neuroscience research: from tank to bedside.
TL;DR: The Zebrafish is emerging as a new important species for studying mechanisms of brain function and dysfunction and the potential of zebrafish models in behavioral phenomics and high-throughput genetic/small molecule screening, which is critical for CNS drug discovery and identifying novel candidate genes.
Journal ArticleDOI
Quicksilver: Fast predictive image registration – A deep learning approach
TL;DR: Quicksilver as mentioned in this paper predicts the momentum-parameterization of LDDMM, which facilitates a patch-wise prediction strategy while maintaining the theoretical properties of LDFMM, such as guaranteed diffeomorphic mappings for sufficiently strong regularization.
Journal ArticleDOI
Schizophrenia, Dopamine and the Striatum: From Biology to Symptoms
TL;DR: This Review draws together developments in neuroimaging techniques that led to the unanticipated finding that dopaminergic dysfunction in schizophrenia is greatest within nigrostriatal pathways, implicating the dorsal striatum in the pathophysiology and calling into question the mesolimbic theory.
References
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Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
Journal ArticleDOI
A review of the optical properties of biological tissues
TL;DR: The known optical properties (absorption, scattering, total attenuation, effective attenuation and/or anisotropy coefficients) of various biological tissues at a variety of wavelengths are reviewed in this article.
Journal ArticleDOI
Visualizing an Olfactory Sensory Map
Peter Mombaerts,Fan Wang,Catherine Dulac,Steve K. Chao,Adriana Nemes,Monica Mendelsohn,James Edmondson,Richard Axel +7 more
TL;DR: A genetic approach is developed to visualize axons from olfactory sensory neurons expressing a given odorant receptor, as they project to the Olfactory bulb, which provides direct support for a model in which a topographic map of receptor activation encodes odor quality in the ofactory bulb.
Journal ArticleDOI
Structural and molecular interrogation of intact biological systems
Kwanghun Chung,Jenelle Wallace,Sung-Yon Kim,Sandhiya Kalyanasundaram,Aaron S. Andalman,Thomas J. Davidson,Julie J. Mirzabekov,Kelly A. Zalocusky,Joanna Mattis,Aleksandra K. Denisin,Sally Pak,Hannah L. Bernstein,Charu Ramakrishnan,Logan Grosenick,Viviana Gradinaru,Karl Deisseroth +15 more
TL;DR: It is shown that CLARITY enables fine structural analysis of clinical samples, including non-sectioned human tissue from a neuropsychiatric-disease setting, establishing a path for the transmutation of human tissue into a stable, intact and accessible form suitable for probing structural and molecular underpinnings of physiological function and disease.
Journal ArticleDOI
Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Jean Livet,Tamily A. Weissman,Hyuno Kang,Ju Lu,Robyn A Bennis,Joshua R. Sanes,Jeff W. Lichtman +6 more
TL;DR: Strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours are presented and may facilitate the analysis of neuronal circuitry on a large scale.